The scientific standards of VectorBuilder’s services are unquestionably high. The web interface to design vectors is intuitive and in fact so easy to use that it can even serve for educational purposes. VectorBuilder has pretty much covered everything one can think about when wanting to design an expression vector, it’s great to have all the versatility on one page. Turnaround time is fast and owing to their professionalism and customer friendly attitude I can recommend VectorBuilder’s services without
Max Planck Institute for Heart and Lung Research, Germany
I have been using ready-to-use lentiviruses from Vectorbuilder and they are working great. I used them against iPSC-derived neurons, and the transduction and expression of transgenes are remarkable. The vector backbones provide multiple options to tag your GOIs with fluorescent proteins or implement antibiotic selection markers. Their vector designing tools are helpful. Customer service is reliable. Good luck!
University of California San Diego, USA
VectorBuilder is an amazing way to save time normally dedicated to molecular biology. The website is easy to use, well-conceived and allows quick design in most cases. For more unusual requests, the team is energetic, reply promptly on every request, and always finds a way to help. Low prices and short timelines really make it possible to consider VectorBuilder for many cloning matters.
Novartis (Basel), Switzerland
We ordered adenovirus from VectorBuilder to overexpress our protein of interest in primary mouse hepatocytes. The ordering process, including vector design, was very fast and easy. We received the plasmid and adenovirus within the time frame that was stated during the ordering process and the transduction efficiency of the adenovirus was very high, enabling us to use a low MOI. As a bonus, we also received the control Ad-EGFP adenovirus for free. In the future, I will not use any more siRNA or classical transfection methods on primary mouse hepatocytes, just adenoviruses from VB! I can highly recommend VectorBuilder's services for vector design and adenovirus production.
University of Würzburg, Germany
I would like to share with you all my experience with VectorBuilder. I do a lot of vector designs for different applications. VectorBuilder website is easy to use and offers efficient and fast vector design tools. In addition, VectorBuilder team is friendly and is open to accommodate special custom and bespoke requirements for vector modifications that are not readily available on the website. Finally, the price is more than reasonable, and I would recommend checking VectorBuilder website for cloning projects.
Wellcome Trust/CRUK Gurdon Institute, University of Cambridge, UK
I used the lentivirus packaging service from VectorBuilder, and the process is very easy. All I needed to do is to ship a small quantity of transfer plasmid, and VectorBuilder will do the rest. Then I received the high titer virus at the end.
UT MD Anderson Cancer Center, USA
We began using VectorBuilder for adenoviral plasmids construction about a year ago. We normally handled all cloning projects ourselves, but when it comes to adenoviral vectors, because of their large size, sometimes the cloning is kind of tricky for inexperienced students. So we decided to give VectorBuilder a try. The plasmid arrived one month later and the virus we generated from it expressed the correct gene. We have ordered more than 10 adenoviral plasmids and they all work fine. VectorBuilder has saved us a lot of time.
DUKE-NUS Graduate Medical School, Singapore
I’ve been using VectorBuilder’s virus packaging services for two years. Their viruses worked well and my projects are going on well. I’ve published two papers on SCI journals using VectorBuilder’s services. Thank you, VectorBuilder!
Zhejiang University, China
We have ordered quite a few plasmids from VectorBuilder in the past 1-2 years, ranging from regular expression plasmids, P
iggyBac, and AAV vectors. The products have been delivered on time and worked as expected. Highly recommend!
Janelia Research Campus of Howard Hughes Medical Institute, USA
We recently purchased a lentiviral plasmid for constitutive expression of GFP and luciferase and the corresponding lentivirus from VectorBuilder. We have infected several tumor cell lines with excellent results. The lentiviral stocks worked very nicely, saving us a lot of time. We are now using these modified cell lines for monitoring tumor growth in animal models using in vivo imaging.
Research institute for Rare Diseases, Spain
I ordered a plasmid vector two years ago. The design on the web platform was easy. Contacts with the specialists to arrange further details were excellent: they were very supportive!
Consiglio Nazionale delle Ricerche, Italy
I would like to share with you my positive experience purchasing a lentiviral vector from VectorBuilder.
First, I am an immunologist and have little experience with cloning. In my recent project, we needed to overexpress a gene in melanoma cells. The transfection efficiency using plasmids in this cell line was extremely low, less than 10%. Then, we tried to use antibiotics to select transfected cells and establish stably expressing cell lines. Unfortunately, for unknown reasons, the ‘stable transfectants’ lost the transfected genes! I was frustrated at this
point,until I met VectorBuilder at the EACR conference in Amsterdam. Using the user-friendly website from VectorBuilder, I designed for the first time in my life my own lentiviral vector and got the product in less than one month. My group leader, who has a lot of cloning experience, could not believe that it worked so quickly. Of course, most important are the experimental results. To access the transduction efficiency, I included an EGFP tag in the vector. Now, I can transduce the melanoma cells with nearly 100% positivity. In the following assays, the genes transduced into the tumor cells resulted in a 6-fold induction in tumor immunogenicity.
Taken together, I strongly recommend VectorBuilder to researchers, especially those who are not so experienced with
cloning,and am convinced that the service and product quality from VectorBuilder can further help me to proceed with my own projects more effectively.
I have been consistently using VectorBuilder over the last couple of years and have been extremely satisfied with the vectors they've delivered. These include standard over-expression vectors, and Cas9 vectors on the PiggyBac backbone. All of the vectors I have purchased so far have passed my QC and provided reproducible functional data.
Vancouver Prostate Centre, Canada
VectorBuilder did vector construction and lentivirus packaging for my shRNA knockdown experiment. The transduction efficiency of the lentivirus was high. Their staff were very considerate, and related technical questions were addressed quickly. I’ve finished many experiments and I’m preparing the manuscript for submission.
School of Nature Conservation, Beijing Forestry University, China
Amazing experience with VectorBuilder! I ordered plasmids at the beginning of December, and despite the holiday
seasonthey arrived exactly as scheduled. Furthermore, customer support reached out to ask what would be my desired delivery date, considering potential holiday conflicts at my institute. Overall, I am very satisfied, would highly recommend VectorBuilder (which I already do) and will buy from them again. All the best from my side!
Medical University of Graz, Austria
VectorBuilder is efficient and easy to use for the outsourcing of plasmid generations. We used the PiggBac system for transgenic modifications and we have had great success.
University of California San Diego, USA
Using VectorBuilder has transformed the way I do my research. VectorBuilder’s easy-to-use web interface allows me to custom build my own plasmids, freeing up time that I would have wasted cloning and instead letting me focus on the research that really matters. They offer great prices with a fast turnaround time. Their friendly and well-informed technical support team are on hand every step of the way, should you need any help. I would strongly recommend VectorBuilder to anyone wanting to accelerate the progress of their research.
David M Brown
University of Nottingham, UK
I’ve used VectorBuilder’s lentivirus packaging service. The virus titer was as promised. Strong fluorescence was observed after transduction. The gene knockdown efficiency was also satisfactory.
Southern Medical University, China
I have been using VectorBuilder's cloning service for several years and am extremely happy with their products and services. Their online design software is very easy to use and provides the most cutting edge solutions for today's most demanding molecular cloning needs. Their customer service is outstanding, with great technical competence and the delivery of my vectors was on time. I have saved months of precious research time by using VectorBuilder. I would recommend VectorBuilder to anyone who wants the best vector design and molecular cloning services.
Northeastern University, USA
Thumbs up! I chose VectorBuilder after comparing it with other companies, because it provides high-quality products and services at unbeatable prices.
The First Affiliated Hospital of Guangzhou Medical University, China
We recently purchased a number of customized plasmids on VectorBuilder. They consisted of expression vectors of two or more ORFs. Our experience was satisfactory, efficient and cost-effective.
The designing stage was facilitated by the VectorBuilder design tool online, in which many of the most commonly used sequences, like fluorescent proteins, selection drug resistance genes, and fusion or cleavage linkers were readily offered to be picked from the ORF lists. Regarding our specific sequences, once pasted into the corresponding window of the design tool, they were immediately matched with the International Nucleotide Sequence Database Collaboration (DDBJ/EMBL/GenBank) accession numbers and compared to the databases. Where there were discrepancies, we were asked which transcripts/cDNAs we preferred to use.
After completing the order, in a matter of three to four weeks, we had in our hands 8 of the 10 plasmids, as offered. For the rest of the
plasmidswe provided the template for a specific sequence. Before proceeding with the cloning, they sequenced it, detected a mutation and corrected it at a low extra cost upon our approval. A week later these corrected plasmids were delivered as well. During the whole process, VectorBuilder’s European team kept a personalized email dialogue with us, enabling us to follow-up and make decisions or accept suggestions about the plasmids.
We have satisfactorily tested every plasmid now. We think that our decision to work with VectorBuilder has boosted our research by saving us time, resources and enabling us to concentrate more on our experiments.
instituteof Technology, Germany
We requested a custom AAV (4-YF) from VectorBuilder. The ordering process was easy and the customer support team were really helpful. Our virus had the expected titer and worked extremely well for our chosen application.
The University of Manchester, UK
VectorBuilder has helped with my research progress. Cloning hasn't been an issue in our lab until recently. Their construct worked well. They saved me a lot of time and provide great services!
University of California, Riverside, USA
I have been successfully cloning for 3+ years, but the last time we tried generating KO cell lines with CRISPR/Cas9 vectors, we were not successful. This was upsetting, especially since I invested a lot of time in these efforts. Then, by chance, I attended one of VectorBuilder's seminars. Due to their reasonable prices and the fact I could focus my time on other work, we decided to give their lentiviral (dual gRNA) Cas9 plasmid a try. The turnaround time was reasonable, their customer service was excellent and they actually gave feedback about the status of my cloning projects. Their website is also easy to use. But most importantly: using their Cas9 dual
gRNAvector, I was able to successfully generate KO cell lines!
I recommend VectorBuilder to CRISPRCas9 users without any hesitation.
Anna Salamero Boix
Georg-Speyer-Haus Frankfurt, Germany
We have ordered several vectors from VectorBuilder, including regular expression plasmids, targeting vectors and AAVs. The constructs were delivered on time and worked very well. They saved us a lot of time and money. Their design team pays attention to detail and made very useful suggestions. Their customer service is excellent as well. Overall, we highly recommend VectorBuilder.
University of California, Riverside, USA
CRISPRcas9 nickase in our study. VectorBuilder helped us design the gRNAs and construct plasmids with double gRNAs and double selection markers (EGFP/puromycin). The gRNA specificity is quite good, better than available online design tools. We got the knockout cell lines in the first run of electroporation with no off-target events. I would recommend VectorBuilder to CRISPR cas9 users.
University Clinic Cologne, Germany
Our laboratory has had great success with both viral constructs and ready-to-use virus prepared by VectorBuilder. They provide excellent customer service at a reasonable price, and their website is extremely user-friendly. Very pleased we discovered this company.
Weill Cornell Medical College, USA
I’ve always been using VectorBuilder’s vector construction service. I’m generally satisfied with the quality of its service, and the problems I encountered were solved quickly.
The First Affiliated Hospital, Sun Yat-sen University, China
VectorBuilder’s service makes designing vectors incredibly simple and there’s no software required, it can all be done through their easy to use website. The lentivirus production service is a massive bonus as it’s so quick and reasonably priced. There’s really no reason to make our own lentivirus anymore. Thanks for a fantastic service.
TC Biopharm, UK
VectorBuilder has provided incredible value and customer service throughout the entire process from product selection, custom design assistance, order submission, status reports and ultimate delivery management. The custom products I purchased from them performed exactly as advertised and in fact surpassed my expectations. I would recommend VectorBuilder to anyone requiring this type of product.
Alloplex Biotherapeutics, USA
I’ve used VectorBuilder’s vector construction and lentivirus packaging services. The turnaround time was short and everything worked well. Also, I received
quickresponses from the customer support team.
Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, China
VectorBuilder is an amazing way to save my time for the generation of my plasmid and provide excellent downstream services. You can get any custom designed vectors you want at a very good price. I am sure I will order the vectors I need in the near future.
Houston Methodist Research Institute, USA
I purchased a lentivirus vector and received it in less than two weeks. I am so glad that I found VectorBuilder, so I can design my lentiviral vector by picking any promotor, and from a variety of antibiotic selection markers and fluorescent tags. Upon transducing several cell lines, my target genes were highly and stably expressed with expression persisting for at least after one month after undergoing selection.
H. Lee Moffitt Cancer Center & Research Institute, USA
VectorBuilder is an intuitive tool for developing vectors at affordable prices. I was in need of a CRISPR lentiviral system with a Puro selection cassette due to working with delicate cells that cannot handle FACS sorting pressures. With their online interface, it was easy to construct the vector I wanted and received the vector in a timely manner! It was simple to produce virus and test it in our cell culture and generated clonal cells with good gene knockdown. I definitely will be ordering more vectors from VectorBuilder to finish up my thesis!
University of Colorado, USA
VectorBuilder has provided very satisfactory service in making various AAV vector constructs for us. The new sales representative is very proactive in offering help on explaining and introducing other VectorBuilder service to us. Expecting to get more support and service from VectorBuilder.
University of Texas Medical School at Ho, USA
We rely on VectorBuilder for high-quality molecular biology reagents. The constructs and particles are delivered to us in a timely manner. They have been more than willing to generate new plasmids and viruses if they do not work well. In summary, we have been very satisfied with VectorBuilder.
Oklahoma Medical Research Foundation, USA
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Modeling of TREX1-Dependent Autoimmune Disease using Human Stem Cells Highlights L1 Accumulation as a Source of Neuroinflammation.