I would like to share with you my positive experience purchasing a lentiviral vector from VectorBuilder.
First, I am an immunologist and have little experience with cloning. In my recent project, we needed to overexpress a gene in melanoma cells. The transfection efficiency using plasmids in this cell line was extremely low, less than 10%. Then, we tried to use antibiotics to select transfected cells and establish stable transfectants. Unfortunately, for unknown reasons, the ‘stable transfectants’ lost the transfected genes! I was frustrated at this point, until I met VectorBuilder at the EACR conference in Amsterdam. Using the user-friendly website from VectorBuilder, I designed for the first time in my life my own lentiviral vector and got the product in less than one month. My group leader, who has a lot of cloning experience, could not believe that it worked so fast. Of course, most important are the experimental results. To access the transduction efficiency, I built an EGFP fragment in the vector. Now, I can transduce the melanoma cells with nearly 100% positivity. In the following assays, the genes transduced into the tumor cells resulted in a 6-fold induction in tumor immunogenicity.
Taken together, I strongly recommend VectorBuilder to researchers, especially those who are not so experienced with cloning, and am convinced that the service and product quality from VectorBuilder can further help me to proceed with my own projects more effectively.
Dr. Fang Zhao
I have been successfully cloning for 3+ years, but the last time I tried generating KO cell lines with CRISPR/Cas9 vectors, we were not successful. This was upsetting, especially since I invested a lot of time in these efforts. Then, by chance I attended to one of the seminars of VectorBuilder. Due to their reasonable prices and the fact I could focus my time on other work, we decided to give their lentiviral (dual gRNA) Cas9 plasmid a try. The turnaround time was reasonable, their customer service was excellent and they really gave feedback about the status of the cloning. Their website is also easy to use. But most importantly: using their Cas9 dual gRNA vector, I was able to successfully generate KO cell lines!
I recommend VectorBuilder to CRISPRCas9 users without any hesitation.
Anna Salamero Boix – PhD Student
Georg-Speyer-Haus Frankfurt, Germany
We recently purchased a number of customized plasmids on VectorBuilder. They consisted of expression vectors of two or more ORFs. Our experience was satisfactory, efficient and cost-effective.
The designing stage was facilitated by the VectorBuilder design tool online, in which many of the most commonly used sequences, like fluorescent proteins, selection drug resistance genes, and fusion or cleavage linkers were readily offered to be picked from the ORF lists offered. Regarding our specific sequences, once pasted into the corresponding window of the design tool, they were immediately matched with the International Nucleotide Sequence Database Collaboration (DDBJ/EMBL/GenBank) accession numbers and compared to the databases. Upon discrepancies, we were asked which one to use.
After completing the order, in a matter of three to four weeks, we had in our hands 8 of the 10 plasmids, as offered. For the rest of the plasmids we provided the template for a specific sequence. Before proceeding with the cloning, they sequenced it, detected a mutation and corrected it at a low extra cost upon our approval. A week later these corrected plasmids were delivered as well. During the whole process the VectorBuilder’s European team kept a personalized dialogue per e-mail with us, enabling us to follow-up and make decisions or accept suggestions about the plasmids.
We have satisfactorily tested every plasmid now. We think that our decision to work with VectorBuilder has boosted our research by saving us time, resources and enabling us to concentrate in our experiments.
Leonel Munoz, MD. PhD.
The Karlsruhe institute of Technology
We requested a custom AAV (4-YF) from VectorBuilder. The ordering process was easy and the customer support team were really helpful. Our virus had the expected titre and worked extremely well for our chosen application.
Dr Jessica Rodgers
The University of Manchester, UK
We applied CRISPR cas9 nickase in our study. VectorBuilder helps us to design the gRNAs and construct the plasmid with double gRNAs and double selection markers (EGFP/puromycin). The gRNA specificity is quite good, better than available online design tools. We got the knockout cell lines in the first run of electroporation with no off-target event. I would recommend VectorBuilder to CRISPR cas9 users.
University Clinic Cologne, GER
Our laboratory has had great success with both viral constructs and ready-to-use virus prepared by VectorBuilder. They provide excellent customer service at a reasonable price, and their website is extremely user-friendly. Very pleased we discovered this company.
Weill Cornell Medical College, USA
VectorBuilder has provided incredible value and customer service throughout the entire process from product selection, custom design assistance, order submission, status reports and ultimate delivery management. The custom products I purchased from them performed exactly as advertised and in fact surpassed my expectations. I would recommend VectorBuilder to anyone requiring this type of product.
Alloplex Biotherapeutics, USA
We ordered adenovirus from VectorBuilder to overexpress our protein of interest in primary mouse hepatocytes. The ordering process, including vector design, was very fast and easy. We received the plasmid and adenovirus within the time frame that was stated during the ordering process and the transduction efficiency of the adenovirus was very high, enabling us to use a low MOI. As a bonus, we also received the control Ad-EGFP adenovirus for free. In the future, I will not use any more siRNA or classical transfection methods on primary mouse hepatocytes, just adenoviruses from VB! I can highly recommend VectorBuilder's services for vector design and adenovirus production.
University of Würzburg, Germany
VectorBuilder is an intuitive tool to help your need in developing vectors at an affordable price. I was in need of a CRISPR Lentiviral system with a Puro selection due to working with delicate cells that cannot handle FACS sorting pressures. With their online interface, it was easy to construct the vector I wanted and received the vector in a timely manner! It was simple to produce virus and test it in our cell culture and had good knockdown clones. I definitely will be ordering more vectors from Cyagen to finish up my thesis!
University of Colorado, Denver
Amazing experience with VectorBuilder! Ordered plasmids beginning of December, and despite the holiday season they arrived exactly as scheduled. Furthermore, customer support reached out to ask what would be my desired delivery date, considering potential holiday time at my institute. Overall, I am super satisfied, would highly recommend VectorBuilder (which I already do) and will buy from them again. All the best from my side!
Medical University of Graz, Austria
We began to use VectorBuilder to prepare adenoviral plasmids about a year ago. We normally handled all the cloning stuffs ourselves, but when it comes to adenoviral vectors, because of its large size, sometimes it is kind of tricky for inexperienced students. We decided to give a try on VectorBuilder. One month later, the plasmid arrived and it generated virus that expressed the correct gene. After that, we have ordered more than 10 adenoviral plasmids and they all work fine. VectorBuilder did save us a lot of time.
DUKE-NUS Graduate Medical School, Singapore
I ordered a plasmid vector two years ago. The design on the web platform was easy. Contacts with the specialists to arrange further details were excellent: they are very supportive!
Consiglio Nazionale delle Ricerche, Italia
I have been using VectorBuilder's cloning service for several years, and am extremely happy with their products and service. Their online design software is very easy to use and provides the most cutting edge solutions for today's most demanding molecular cloning needs. Their customer service is outstanding, with great technical competence and is always on time to delivery the excellent results, which have saved me months of precious research time. I would recommend VectorBuilder to anyone who want the best vector design and molecular cloning service.
Northeastern University, USA
We have ordered quite a few plasmids from VectorBuilder in the past 1-2 years, ranging from regular plasmids, piggybac, and AAV vectors. The products have been delivered on time and worked as expected. Highly recommend!
Janelia Research Campus of Howard Hughes Medical Institute, USA
Using VectorBuilder has transformed the way I do my research. VectorBuilder’s easy-to-use web interface allows me to custom build my own plasmids, freeing up time that I would have wasted cloning and instead letting me focus on the research that really matters. They offer great prices with a fast turnaround time. Their friendly and well-informed technical support team are on hand every step of the way, should you need any help. I would strongly recommend VectorBuilder to anyone wanting to accelerate the progress of their research.
David M Brown
University of Nottingham, UK
The scientific standards of VectorBuilder’s services are unquestionably high. The web interface to design vectors is intuitive and in fact so easy to use that it can even serve for educational purposes. VectorBuilder has pretty much covered everything one can think about when wanting to design an expression vector, it’s great to have all the versatility on one page. Turnaround time is fast and owing to their professionalism and customer friendly attitude I can recommend VectorBuilder’s services without reserve.
Max Planck Institute for Heart and Lung Research, Germany
I’ve been using VectorBuilder’s virus packaging services for two years. The virus worked well and my projects are going on well. I’ve published two papers on SCI journals using VectorBuilder’s services. Thank you, VectorBuilder!
Zhejiang University, China
We recently purchased from VectorBuilder a lentiviral plasmid for constitutive expression of GFP and luciferase and the corresponding lentivirus. We had infected several tumor cell lines with excellent results. The lentiviral stocks worked very nice saving us a lot of time. We are now using these modified cell lines for monitoring tumor growth in animal models using in vivo imaging.
Research institute for Rare Diseases, Spain
VectorBuilder did vector construction and lentivirus packaging for my shRNA knockdown experiment. The transduction efficiency of the lentivirus was high. Their staffs were very considerate, and related technical questions were addressed quickly. I’ve finished many experiments and I’m preparing the manuscript for submission.
School of Nature Conservation, Beijing Forestry University, China
VectorBuilder’s service makes designing vectors incredibly simple and there’s no software required, it can all be done through their easy to use website. The lentivirus production service is a massive bonus as it’s so quick and reasonably priced there’s really no reason to make our own lentivirus anymore. Thanks for a fantastic service.
TC Biopharm, UK
Thumbs up! I chose VectorBuilder after comparing it with other companies, because it provides high-quality products and services at unbeatable price.
The First Affiliated Hospital of Guangzhou Medical University, China
VectorBuilder is an amazing way to save time on molecular biology. The web site is easy to use, well-conceived and allows quick design in most cases. For more unusual requests, the team is energetic, reply promptly on every request, and always finds a way to help. Low prices and short timelines really make it possible to consider VectorBuilder on many cloning matters.
Novartis (Basel), Switzerland
I’ve used VectorBuilder’s vector construction and lentivirus packaging services. The turnaround time was short and everything worked well. Also, I got quick response from the customer support team.
Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, China
I’ve used VectorBuilder’s lentivirus packaging service. The virus titer was as promised. Strong fluorescence was observed after transduction. The gene knockdown efficiency was satisfied.
Southern Medical University, China
I’ve always been using VectorBuilder’s vector construction service. I’m generally satisfied with the quality of its service, and the problems I encountered were solved quickly.
The First Affiliated Hospital, Sun Yat-sen University, China
I have been consistently using VectorBuilder over the last couple years and have been extremely satisfied with the vectors delivered to me. These include standard over-expression vectors, and Cas9 vectors on piggybac backbone. All of the vectors I have purchased so far have passed my QC and provided reproducible functional data.
Vancouver Prostate Centre, Canada
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Lis R et al.
Nature. 545: 439 (2017)
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Cell Stem Cell. 21: 319 (2017)
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