Drug Selection Cassettes

VectorBuilder offers many popular vector components that users can choose from when designing their vectors. The tables below provide detailed information on these popular components, which are listed separately by category.
Selection Cassettes
Name Description Application Notes Selection Drug References Sequence
Neo-cassette mPGK promoter driving Neo with SV40 late pA Allows cells to be resistant to geneticin (G418). Geneticin (G418) Engineered by VectorBuilder View
Puro-cassette mPGK promoter driving Puro with SV40 late pA Allows cells to be resistant to puromycin. Puromycin Engineered by VectorBuilder View
LoxP-Neo-LoxP mPGK promoter driving Neo with SV40 late pA, flanked by LoxP Allows cells to be resistant to geneticin (G418); can be deleted by Cre recombinase. Geneticin (G418) Engineered by VectorBuilder View
FRT-Neo-FRT mPGK promoter driving Neo with SV40 late pA, flanked by FRT Allows cells to be resistant to geneticin (G418); can be deleted Flp recombinase. Geneticin (G418) Engineered by VectorBuilder View
LoxP-Puro-LoxP mPGK promoter driving Puro with SV40 late pA, flanked by LoxP Allows cells to be resistant to puromycin; can be deleted by Cre recombinase. Puromycin Engineered by VectorBuilder View
FRT-Puro-FRT mPGK promoter driving Puro with SV40 late pA, flanked by FRT Allows cells to be resistant to puromycin; can be deleted by Flp recombinase. Puromycin Engineered by VectorBuilder View
3xP3-DsRed DsRed fluorescent marker driven by 3xP3 promoter Used for identification of genetically engineered fly lines. - Insect Biochem Mol Biol. 32:1221 (2002) Ver 1 Ver 2