Vector Construction

As the world's largest provider of custom vector cloning services, VectorBuilder can make virtually any vector tailored to your research needs. Over 100 vector systems on our platform have been optimized through extensive R&D work, and all have been validated by in vitro and, where applicable, in vivo testing. Besides cloning, we also offer many related services such as virus packaging, library construction, BAC modification, stable cell line generation, plasmid DNA extraction, mutagenesis, and RNA preparation.
How do I obtain vector construction services?
You can obtain cloning services in one of the three ways on VectorBuilder’s homepage:
Design your custom vectors using our highly intuitive online vector design tool in just a minute or two.
Submit your needs to us by describing in words. Normally within a day or two, our experienced scientists will send you the vector design for free.
Directly search our standard gene expression and shRNA vectors by gene name.
View finished vector design
Add vector construction and related services (e.g. virus packaging) into shopping cart

By default, we ship vectors in the format of E. coli stock. Upon request, small amount of plasmid DNA can be provided if available. Two scales of high-quality plasmid DNA can be purchased when adding vector construction service into shopping cart: Miniprep (> 10 ug) and Maxiprep (> 300 ug, endotoxin-free, sterile).

Quality control

All of VectorBuilder constructed vectors have 100% sequence guarantee. Our vectors undergo stringent quality control to ensure that they contain the correct sequence exactly as designed. Common QC includes DNA quantification, A260/280 measurement, restriction enzyme digestion, Sanger sequencing, recovering bacteria from glycerol stock, and re-transformation.

Price and turnaround time
VectorBuilder offers top-quality vector construction services at unbeatable price with rapid turnaround time. Outsourcing cloning to us is often cheaper than the cost of reagents you will need to do it yourself, let alone savings on your time, effort and headaches. Price and turnaround time are based on the criteria below.
These are vectors built from VectorBuilder's standard backbones and standard components.
Price and turnaround time for basic vectors:
Vector Type Price (USD) Turnaround*
shRNA vector $99 7-14 days
gRNA vector (single-gRNA) $99 7-14 days
gRNA vector (dual-gRNA) $289 13-21 days
Expression vector $159 10-16 days

* Turnaround time is defined as the time from when an order is accepted to when it is shipped. It does not include waiting time for any materials that the customer needs to supply (e.g. template DNA). Adenoviral vectors may have longer turnaround time.

These are vectors using VectorBuilder's standard backbones, but which contain complex components that need to be created. They typically require multiple steps to build, and often involve complicated procedures such as amplification of large fragments from template DNA, fusion of multiple fragments, de novo gene synthesis, and targeted mutagenesis.

Additional price and turnaround time for complex vectors:
Cloning Procedure Additional Price (USD) Additional Turnaround
Insertion of one PCR fragment (≤6 kb) $80 5-10 days
Insertion of two fused PCR fragments (≤6 kb) $100 5-11 days
Insertion of three fused PCR fragments (≤6 kb) $130 5-12 days
Insertion of four fused PCR fragments (≤6 kb) $160 5-14 days
Insertion of five fused PCR fragments (≤6 kb) $180 5-19 days
De novo gene synthesis (≤1.2 kb) $0.22/bp + processing fee* 6-10 days
De novo gene synthesis (1.2-2.4 kb) $0.22/bp + processing fee* 10-14 days
De novo gene synthesis (2.4-3.6 kb) $0.25/bp + processing fee* 10-16 days
De novo gene synthesis (3.6-5 kb) $0.27/bp + processing fee* >16 days
Mutagenesis $100/site 7-9 days

* Processing fee and longer turnaround may apply when the fragment contains regions that are difficult to synthesize such as high GC content, simple repeats or segmental repeats.

These are vectors built from backbones provided by the customer rather than using our own standard backbones. The customer-supplied backbone is first validated by restriction digest and sequencing of key regions before cloning. Prices for cloning non-standard vectors are considered on a case-by-case basis.

Customer-supplied materials

If customer-supplied materials such as backbone or gene template is needed, please send us the materials following the Materials Submission Guidelines. Please strictly follow our guidelines to set up shipment to avoid any delay or damage of materials. All customer-supplied materials undergo mandatory QC by VectorBuilder which may incur $100 surcharge. Please note that production would not be initiated until all customer-supplied materials are received and pass QC.

Premade vectors

In addition to custom vector construction, VectorBuilder offers a variety of premade vectors which are often used as control or helper vectors.


Please refer to our Vector FAQ for commonly asked technical questions. To get additional information on our offerings, please contact VectorBuilder service team following the contact information here.