Virus Packaging

VectorBuilder has developed a range of proprietary technologies and reagents that greatly improved virus packaging protocols in terms of titer, purity, viability and consistency. Our packaging protocols are also optimized for the viral vector systems used in our cloning services. As a result, we have a growing base of highly satisfied customers who come back to us time after time for their cloning and virus packaging needs.
How do I obtain virus packaging services?
You can obtain packaging services as follows:
  • You can package viruses using vectors that are custom built by VectorBuilder. When you put the cloning service for such a vector in your shopping cart, you will have the option to also add the corresponding virus packaging service to the cart.
  • To package viruses using your own vectors, just submit your needs to us by clicking the Send Design Request button on the homepage. Our scientists will send you a link to a service proposal including price, turnaround and other relevant information. You can add the proposal to your shopping cart.
  • Lastly, you can place an order for the content of your shopping cart by following instructions there.
Types of packaging services offered
Currently, we offer packaging services for the following virus types:
We will consider packaging other types of viruses per your need on a case-by-case basis.
You can read more about our standard lentivirial, adenoviral, AAV and MMLV vector systems by going to menu item Learning CenterGuide to Vector Systems.
Price and turnaround time
Due to our technology advantage, our virus packaging services have the highest quality in the industry and are also faster and more affordable than other suppliers. All custom-packaged viruses undergo thorough testing to ensure that they meet the purity, viability and titer requirements.
Price and turnaround time for virus packaging:
Virus Type Scale Application Titer & Volume Price (USD) Turnaround*
Lentivirus
(including regular and non-integrating)
Pilot Cell culture >108 TU/ml, 250ul $399 7-14 days
Medium Cell culture >108 TU/ml, 1 ml $599 7-14 days
Large Cell culture >109 TU/ml, 1 ml $999 7-14 days
Ultra-purified medium In vivo >109 TU/ml, 500ul $1199 7-14 days
Ultra-purified large In vivo >109 TU/ml, 1 ml $1499 7-14 days
Adenovirus Pilot Cell culture >1010 PFU/ml, 250ul $399 20-32 days
Medium Cell culture >1010 PFU/ml, 1 ml $599 20-32 days
Large Cell culture >1011 PFU/ml, 1 ml $999 20-32 days
Ultra-purified In vivo >1012 VP/ml, 1 ml $1499 22-36 days
AAV Pilot Cell culture >1011 GC/ml, 250ul $399 15-25 days
Medium Cell culture >1011 GC/ml, 1 ml $599 15-25 days
Large Cell culture >1012 GC/ml, 1 ml $999 15-25 days
Ultra-purified pilot In vivo >1013 GC/ml, 100ul $1099 20-30 days
Ultra-purified medium In vivo >1013 GC/ml, 500ul $1699 20-30 days
MMLV Pilot Cell culture >107 TU/ml, 250ul $399 7-14 days
Medium Cell culture >107 TU/ml, 1 ml $599 7-14 days
Large Cell culture >108 TU/ml, 1 ml $999 7-14 days
Ultra-purified In vivo >108 TU/ml, 1 ml $1499 7-14 days

* Turnaround time is defined as the time from when an order is accepted to when it is shipped. It does not include waiting time for any materials that the customer needs to supply (e.g. viral vectors).

Control virus
For each order of custom-packaged virus (ultra-purified virus excluded), VectorBuilder will provide free control virus for testing transduction. The control virus is designed to match the biological application of the custom virus. For example, if the custom virus overexpresses a gene, then the control virus provided will overexpress EGFP, and if the custom virus expresses an shRNA against a gene, then the control virus provided will express a scramble shRNA. For ultra-purified custom virus, VectorBuilder provides ultra-purified control virus available for purchase at very low cost. 
The detailed information of our free control virus is shown below:
Vector System Control Vector Name Control Vector ID

Custom Virus Packaging

Control Virus Amount Application
Lentivirus gene expression pLV[Exp]-EGFP:
T2A:Puro-EF1A>mCherry
VB160109-10005 Pilot-scale >108 TU/ml, 100ul in HBSS Cell culture
Medium-scale >108 TU/ml, 2x100ul in HBSS Cell culture
Large-scale >108 TU/ml, 2x100ul in HBSS Cell culture
Lentivirus U6-based shRNA knockdown

pLV[shRNA]-EGFP:
T2A:Puro-U6>Scramble_shRNA

VB151023-10034 Pilot-scale >108 TU/ml, 100ul in HBSS Cell culture
Medium-scale >108 TU/ml, 2x100ul in HBSS Cell culture
Large-scale >108 TU/ml, 2x100ul in HBSS Cell culture
Lentivirus miR30-based shRNA knockdown

pLV[miR30-shRNA]-EGFP:T2A:Puro-EF1A>
mCherry:Scramble
[miR30-shRNA]

VB180124-1174nde Pilot-scale >108 TU/ml, 100ul in HBSS Cell culture
Medium-scale >108 TU/ml, 2x100ul in HBSS Cell culture
Large-scale >108 TU/ml, 2x100ul in HBSS Cell culture
Lentivirus CRISPR system

pLV[Exp]-EGFP:T2A:
Puro-EF1A>mCherry

VB160109-10005 Pilot-scale >108 TU/ml, 100ul in HBSS Cell culture
Medium-scale >108 TU/ml, 2x100ul in HBSS Cell culture
Large-scale >108 TU/ml, 2x100ul in HBSS Cell culture
Adenovirus gene expression pAV[Exp]-CMV>EGFP VB150925-10024 Pilot-scale >1010 PFU/ml, 100ul in HBSS Cell culture
Medium-scale >1010 PFU/ml, 2x100ul in HBSS Cell culture
Large-scale >1010 PFU/ml, 2x100ul in HBSS Cell culture
Adenovirus U6-based shRNA knockdown pAV[shRNA]-EGFP-U6>
Scramble_shRNA
VB161026-1127kyf Pilot-scale >1010 PFU/ml, 100ul in HBSS Cell culture
Medium-scale >1010 PFU/ml, 2x100ul in HBSS Cell culture
Large-scale >1010 PFU/ml, 2x100ul in HBSS Cell culture
Adenovirus miR30-based shRNA knockdown

pAV[miR30-shRNA]-CMV>EGFP:
Scramble[miR30-shRNA]

VB180124-1101mjs Pilot-scale >1010 PFU/ml, 100ul in HBSS Cell culture
Medium-scale >1010 PFU/ml, 2x100ul in HBSS Cell culture
Large-scale >1010 PFU/ml, 2x100ul in HBSS Cell culture
Adenovirus CRISPR system pAV[Exp]-CMV>EGFP

VB150925-10024

Pilot-scale >1010 PFU/ml, 100ul in HBSS Cell culture
Medium-scale >1010 PFU/ml, 2x100ul in HBSS Cell culture
Large-scale >1010 PFU/ml, 2x100ul in HBSS Cell culture
AAV gene expression pAAV[Exp]-CMV>EGFP VB150925-10026 Pilot-scale >1011 GC/ml, 100ul in PBS Cell culture
Medium-scale >1011 GC/ml, 2x100ul in PBS Cell culture
Large-scale >1011 GC/ml, 2x100ul in PBS Cell culture
AAV U6-based shRNA knockdown pAAV[shRNA]-EGFP-U6>
Scramble_shRNA
VB180117-1020znr Pilot-scale >1011 GC/ml, 100ul in PBS Cell culture
Medium-scale >1011 GC/ml, 2x100ul in PBS Cell culture
Large-scale >1011 GC/ml, 2x100ul in PBS Cell culture
AAV miR30-based shRNA

pAAV[miR30-shRNA]-CMV>EGFP:
Scramble[miR30-shRNA]

VB180124-1104yzn Pilot-scale >1011 GC/ml, 100ul in PBS Cell culture
Medium-scale >1011 GC/ml, 2x100ul in PBS Cell culture
Large-scale >1011 GC/ml, 2x100ul in PBS Cell culture
AAV CRISPR system pAAV[Exp]-CMV>EGFP VB150925-10026 Pilot-scale >1011 GC/ml, 100ul in PBS Cell culture
Medium-scale >1011 GC/ml, 2x100ul in PBS Cell culture
Large-scale >1011 GC/ml, 2x100ul in PBS Cell culture
MMLV gene expression pMMLV[Exp]-EGFP:T2A:Puro VB170316-1032yra Pilot-scale >107 TU/ml, 100ul in HBSS Cell culture
Medium-scale >107 TU/ml, 2x100ul in HBSS Cell culture
Large-scale >107 TU/ml, 2x100ul in HBSS Cell culture