IRES-EGFP IVT circRNA

VectorBuilder’s IRES-EGFP IVT circRNA produces robust GFP expression from a covalently closed RNA formed through intronic splicing. This circRNA has altered degradation kinetics, resulting in prolonged GFP expression compared to conventional mRNA.

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Click here to view the amino acid sequence translated from IRES-EGFP IVT circRNA >>

Shipping and storage

Our IVT circRNA products are stored in a 1 mM sodium citrate buffer (pH 6.4) and can be stored at -80°C for up to 12 months. RNA products are shipped on dry ice and freeze-thaw cycles should be avoided.

Experimental Validation

Figure 1. circRNA encoding EGFP was transfected into HEK293T cells and exhibited long-term expression compared to traditional mRNA.

Gel electrophoresis analysis of precursor and circular EGFP RNA (circRNA-EGFP) after incubation with varying concentrations of RNase A as indicated above. RNAse A will digest all linear but not circular RNA

Figure 2. Gel electrophoresis analysis of precursor and circular EGFP RNA (circRNA-EGFP) after incubation with varying concentrations of RNase A as indicated above. RNase A will digest all linear but not circular RNA.