IRES-EGFP IVT circRNA
VectorBuilder’s IRES-EGFP IVT circRNA produces robust GFP expression from a covalently closed RNA formed through intronic splicing. This circRNA has altered degradation kinetics, resulting in prolonged GFP expression compared to conventional mRNA. In addition, m6A modification can further enhance translational efficiency while reducing immunogenicity, making it well suited for experimental controls requiring sustained expression or for in vivo studies.
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Click here to view the amino acid sequence translated from IRES-EGFP IVT circRNA >>
Shipping and storage
Our IVT circRNA products are stored in a 1 mM sodium citrate buffer (pH 6.4) and can be stored at -80°C for up to 12 months. RNA products are shipped on dry ice and freeze-thaw cycles should be avoided.
Experimental Validation
- Unmodified
- m6A-modified
Figure 1. circRNA encoding EGFP was transfected into HEK293T cells and analyzed by fluorescence microscopy. circRNA-transfected cells exhibited longer-term expression compared to traditional mRNA.
Figure 2. Gel electrophoresis analysis of precursor and circular EGFP RNA (circRNA-EGFP) after incubation with varying concentrations of RNase A as indicated above. RNase A will digest all linear but not circular RNA.
Figure 3. m6A modification enhances circular RNA (circRNA) expression. (A) EGFP circRNA (marked by a pink asterisk) with no or partial N6-methyladenosine (m6A) incorporation was efficiently produced, whereas complete m6A incorporation disrupted circularization. circRNA lacks free ends and therefore migrates more slowly than linear RNA during electrophoresis. (B) circRNA containing 5% m6A showed the highest EGFP expression following transfection of 1 ug RNA into HEK293T cells cultured in 12-well plates. Exposure times: bright field, 200 ms; EGFP, 100 ms.
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Brochures & Flyers
Certificate of Analysis (COA)
Material Safety Data Sheet (MSDS)
