HiExpress™ Luciferase LNP-mRNA

VectorBuilder's sequence-optimized HiExpress™ Firefly Luciferase (Fluc) LNP-mRNA is thoroughly validated for quality and functionality. It serves as an ideal control for a variety of in vitro and in vivo applications.

Ordering Information Price Match

Click here to view the amino acid sequence translated from HiExpress^TM Firefly Luciferease mRNA >>

Shipping and storage

Our LNP-mRNA products are stored in Tris buffer (pH 7.4) and can be stored at -80°C for up to 6 months. RNA products are shipped on dry ice and freeze-thaw cycles should be avoided.

Experimental Validation

HiExpress™ FLuc
GalNAc LNP HiExpress™ FLuc
Mouse Anti-CD31 Conjugated HiExpress™ FLuc

Luciferase activity in HEK293T cells and C57BL/6 mice being transfected with Firefly Luciferase mRNAs.

Figure 1. Firefly Luciferase IVT mRNA expression in vitro and in vivo. (A) Quantified expression of HiExpress™ Firefly Luciferase mRNA and other luciferase mRNAs in HEK293T cells. Cells grown on a 12-well plate were transfected with 0.5 ug of mRNA per well and luciferase activity was measured at 6 h, 24 h, 48 h, and 72 h post transfection. (B) Luciferase activity measured in adult C57BL/6 mice injected intramuscularly with 30 ug of LNP encapsulated mRNA at 6 h, 24 h, and 48 h post injection. FLuc WT indicates wild-type firefly luciferase. FLuc WT (co) indicates codon-optimized wild-type firefly luciferase. FLuc2 indicates Luc2 firefly luciferase.

Figure 2. (A) Characterization of GalNAc and SM102 lipid nanoparticles by dynamic light scattering (Zetasizer). (B) Efficient hepatic delivery of mRNA in Ldlr^−/− mice using a GalNAc-lipid nanoparticle (LNP). HiExpress™ Firefly Luciferase IVT mRNA made with m1Ψ was encapsulated with GalNAc or standard SM102-based LNP. 0.5 mg encapsulated RNA per kg of body weight was intravenously administered to each wild-type or Ldlr knockout C57BL/6 mouse. Luciferase activity was visualized by live imaging at 6 h and 24 h post-injection.

Figure 3. Anti-CD31 conjugated firefly luciferase (FLuc) LNP-mRNA showed improved luciferase expression in lung. Mouse strain: C57BL/6J; mouse age: 6-8 weeks; mouse gender: female; administration route: tail vein. Negative controls: IgG2a-conjugated FLuc LNP-mRNA.