Zebrafish EGFP IVT mRNA
VectorBuilder’s zebrafish EGFP mRNA was generated with optimized polyA tail and Kozak sequence. The high expression of EGFP has been validated both in vitro and in vivo.
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Shipping and storage
Our IVT mRNA products are stored in a 1 mM sodium citrate buffer (pH 6.4) and can be stored at -80°C for up to 12 months. The products are shipped on dry ice and should be avoided for freeze-thaw cycles.
Zebrafish EGFP IVT mRNA expression in vitro and in vivo
(A) 293T cells grown on a 12-well plate were transfected with 1 ug of zebrafish EGFP mRNA per well. Representative images at 72 h post-transfection indicate that 110 nt polyA tail increases EGFP expression in vitro. (B) One-cell stage zebrafish embryos were screened for EGFP expression at 6 h post-microinjection of 250 pg of zebrafish EGFP IVT mRNA. Fluorescent images indicate that optimized Kozak sequence (version 2) increased EGFP expression in vivo.
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