LentiBuilder™ Lentivirus Packaging Kit
As a leading expert in lentiviral gene delivery, VectorBuilder proudly introduces the LentiBuilder™ Lentivirus Packaging Kit, enabling effortless in-house production of high-quality third-generation lentivirus directly at your bench. Built on our proprietary miniVec™ technology and extensive optimizations of the packaging system, the kit delivers exceptional virus titers and transduction results.
Highlights
Ultra-High Titer
Achieve market-leading functional titers (107-108 TU/ml) for regular-sized vectors and reliable yields for oversized vectors.
Ease of Use
Combine packaging plasmids pre-mixed at an optimal ratio with an efficient transfection reagent – two tubes, one powerful system.
Top Compatibility
Experience the best packaging performance by using this kit together with transfer vectors designed in our Vector Design Studio.
Ordering Information
| Product | Catalog No. | Size | Quantity | Price (USD) | Buy Now |
|---|---|---|---|---|---|
| LentiBuilder™ Lentivirus Packaging Kit | LVKT0010 | 10 reactions | {[ qty1 * 559 | areaCurrency:0]} | ||
| LVKT0050 | 50 reactions | {[ qty2 * 1999 | areaCurrency:0]} |
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LentiBuilder™ Lentivirus Packaging Kit!
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Contents
| Catalog No. | Component | Specifications |
|---|---|---|
| LVKT0010 | LentiBuilder™ Packaging Mix | 1 x 80 ul |
| LentiBuilder™ Transfection Reagent | 1 x 500 ul | |
| LVKT0050 | LentiBuilder™ Packaging Mix | 5 x 80 ul |
| LentiBuilder™ Transfection Reagent | 5 x 500 ul |
Shipping and storage
The LentiBuilder™ Lentivirus Packaging Kit is shipped at ambient temperature. Upon receipt, the packaging mix should be stored at -20°C, while the transfection reagent should be stored at 4°C to avoid freeze-thaw cycles. When stored properly, the shelf life of the kit is approximately one year.
Note: The LentiBuilder™ Lentivirus Packaging Kit is for research use only. By purchasing this product, the buyer agrees to be bound by VectorBuilder's Limited Use Label License. Purchasers and users are not permitted to resell, replicate, manufacture, or reverse engineer any kit contents, nor to sequence, amplify, or modify any regions of the DNA plasmids contained within the kit. For the full license terms, please contact us.
Technical Information
Lentivirus is among the most commonly used viral vectors for efficient gene delivery. The resulting permanent genomic integration of your gene of interest (GOI) supports a variety of in vitro, in vivo, and ex vivo applications, such as library screenings, stable cell line generation, CRISPR-based genome editing, and CAR expression. Successful production of third-generation lentivirus requires co-transfecting a transfer plasmid carrying the GOI alongside packaging plasmids encoding Gag/Pol, Rev, and an envelope protein like VSV-G into packaging cells (e.g. 293T cells).
Figure 1. Workflow for lentivirus production using the LentiBuilder™ Lentivirus Packaging Kit.
Based on our extensively optimized packaging system, we have developed the LentiBuilder™ Lentivirus Packaging Kit to make your in-house packaging of virus comparably reliable to the virus produced by VectorBuilder. The kit consists of two components: a packaging plasmid mix that is optimized in ratio and concentration and a reagent for highly efficient transfection of packaging cells. In addition, all packaging plasmids are constructed using our miniVec™ technology, which further improves titer, safety, viability, and consistency of the produced lentiviral vectors.
- Titration
- Transduction
Figure 2. Market-leading functional titers of lentivirus produced from LentiBuilder™ Lentivirus Packaging Kit. A side-by-side comparison of virus packaging was conducted across different packaging systems using several transfer vectors varying in transgene and viral genome size. The same amount of transfer plasmid was used for all systems, along with each system’s recommended amount and ratio of packaging plasmids. Functional titers in 293T cells were determined by qPCR. LentiBuilder™ consistently surpasses major competitors across all vectors, and achieves exceptional titers even for lentivirus with oversized genomes (i.e. >9.2 kb).
Figure 3. Transduction of challenging cell types using lentivirus produced from the LentiBuilder™ packaging system. The data was generated by VectorBuilder’s customers and consented for public use. (A) Primary mouse hippocampal neurons were transduced with a lentiviral shRNA expression vector carrying EGFP as the reporter. Images were taken 5 days post-transduction (MOI=7). (B) Primary human hematopoietic stem cells were transduced with a lentiviral gene overexpression vector carrying EGFP as the reporter. Images were taken 8 days post-transduction (MOI=50). In both groups: exposure time=100 ms; magnification=100x.
Resources
Both our kit and packaging services deliver lentivirus with top-tier quality and market-leading transduction efficiency. The table below summarizes the differences:
| LentiBuilder™ Lentivirus Packaging Kit | Lentivirus Packaging Services | |
|---|---|---|
| Prerequisites | In-lab expertise, experimental platforms, and a BSL-2 environment for lentivirus packaging | None; fully managed by VectorBuilder’s team of experts |
| Virus type | VSV-G pseudotyped 3rd-generation lentivirus |
|
| Technologies |
|
|
| Typical titer | 107–108 TU/ml | 108–1010 TU/ml |
| Quality control (QC) | Various QC assays chosen by the researcher based on purpose, capabilities, and available instruments | Comprehensive QC assays including titer measurement, transduction, and sterility testing; optional qPCR, flow cytometry, and endotoxin testing for enhanced quality assurance |
| Time needed | On-demand, flexible packaging completed in 3 days | Fast and reliable delivery in as few as 6 days, including QC |
| Recommended use | For cell culture (additional purification is recommended for delicate applications) | For cell culture or in vivo applications |
Our kit is specifically designed for packaging VSV-G pseudotyped lentivirus. For lentivirus pseudotyped with other envelope proteins such as GaLV, RD114, BaEV, and coronavirus spike (S) proteins, please refer to our lentivirus packaging services.
| Lentivirus | MMLV | Adenovirus | AAV | |
|---|---|---|---|---|
| Tropism | Broad | Broad | Ineffective for some cells | Depending on viral serotype |
| Can infect non-dividing cells? | Yes | No | Yes | Yes |
| Stable integration or transient | Stable integration | Stable integration | Transient, episomal | Transient, episomal |
| Maximum titer | High | Moderate | High | Very high |
| Promoter customization | Yes | No | Yes | Yes |
| Primary use | Cell culture and in vivo | Cell culture and in vivo | In vivo | In vivo |
| Immune response in vivo | Low | Low | High | Very low |