Premade Control Vectors
From gene overexpression to shRNA knockdown and CRISPR experiments, we offer a wide range of premade control vectors to meet your research needs. For experiment ready viruses please visit our Premade Control Viruses page.
Highlights
Large collection of ready-to-ship premade vectors available in viral and non-viral backbones.
Functionally validated vector backbones and components with 100% sequence guarantee.
Add downstream services, including plasmid DNA preparation and virus packaging in just a few clicks.Fluorescent Reporters
We offer a large collection of premade fluorescent reporter plasmids, enhancing the efficiency of your single color, multicolor and fluorescence energy transfer experiments. For many more fluorescent protein and construct options, please use our popular vectors search function or view our full selection of popular ORFs.
GFP
mCherry
TagBFP2
 Regular Plasmid Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pRP[Exp]-CMV>EGFP | $189.00 | View and Order |
| pRP[Exp]-Puro-CMV>EGFP | $189.00 | View and Order |
| pRP[Exp]-mCherry-CMV>EGFP | $189.00 | View and Order |
| pRP[Exp]-CAG>EGFP | $189.00 | View and Order |
| pRP[Exp]-Puro-CAG>EGFP | $189.00 | View and Order |
| pRP[Exp]-mCherry-CAG>EGFP | $189.00 | View and Order |
| pRP[Exp]-EF1A>EGFP | $189.00 | View and Order |
| pRP[Exp]-Puro-EF1A>EGFP | $189.00 | View and Order |
| pRP[Exp]-mCherry-EF1A>EGFP | $189.00 | View and Order |
 Lentiviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pLV[Exp]-EF1A>EGFP | $189.00 | View and Order |
| pLV[Exp]-Puro-EF1A>EGFP | $189.00 | View and Order |
| pLV[Exp]-mCherry-EF1A>EGFP | $189.00 | View and Order |
| pLV[Exp]-EGFP/Puro-EF1A>mCherry | $189.00 | View and Order |
| pLV[Exp]-CMV>EGFP | $189.00 | View and Order |
| pLV[Exp]-Puro-CMV>EGFP | $189.00 | View and Order |
| pLV[Exp]-TRE>EGFP | $189.00 | View and Order |
| pLV[Exp]-Puro-TRE>EGFP | $189.00 | View and Order |
| pLV[Exp]-Bsd-TRE>EGFP | $189.00 | View and Order |
| pLV[shRNA]-EGFP-U6>Scramble_shRNA | $149.00 | View and Order |
 AAV Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAAV[Exp]-CAG>EGFP | $189.00 | View and Order |
| pAAV[Exp]-CAG>EGFP:WPRE | $189.00 | View and Order |
| pAAV[Exp]-CMV>EGFP | $189.00 | View and Order |
| pAAV[Exp]-CMV>EGFP:WPRE | $189.00 | View and Order |
| pscAAV[Exp]-CMV>EGFP | $189.00 | View and Order |
| pAAV[Exp]-SYN1>EGFP | $189.00 | View and Order |
| pscAAV[Exp]-SYN1>EGFP | $189.00 | View and Order |
| pAAV[Exp]-SYN1>EGFP:WPRE | $189.00 | View and Order |
 Adenoviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAV[Exp]-CMV>EGFP | $189.00 | View and Order |
| pAd5/F35[Exp]-CMV>EGFP | $189.00 | View and Order |
| pAV[Exp]-EF1A>EGFP | $189.00 | View and Order |
| pAd5/F35[Exp]-EF1A>EGFP | $189.00 | View and Order |
 Transposon Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pPB[Exp]-EF1A>EGFP | $189.00 | View and Order |
| pPB[Exp]-Puro-EF1A>EGFP | $189.00 | View and Order |
| pPB[Exp]-CAG>EGFP | $189.00 | View and Order |
| pPB[Exp]-Puro-CAG>EGFP | $189.00 | View and Order |
| pPB[Exp]-CMV>EGFP | $189.00 | View and Order |
| pPB[Exp]-Puro-CMV>EGFP | $189.00 | View and Order |
| pPB[Exp]-TRE>EGFP | $189.00 | View and Order |
| pPB[Exp]-Puro-TRE>EGFP | $189.00 | View and Order |
| pSB[Exp]-EF1A>EGFP | $189.00 | View and Order |
| pSB[Exp]-EF1A>EGFP/Puro | $189.00 | View and Order |
| pSB[Exp]-CMV>EGFP | $189.00 | View and Order |
| pSB[Exp]-CMV>EGFP/Puro | $189.00 | View and Order |
| pSB[Exp]-CAG>EGFP | $189.00 | View and Order |
| pSB[Exp]-CAG>EGFP/Puro | $189.00 | View and Order |
| Regular Plasmid Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pRP[Exp]-CMV>mCherry | $189.00 | View and Order |
| pRP[Exp]-Puro-CMV>mCherry | $189.00 | View and Order |
| pRP[Exp]-EGFP-CMV>mCherry | $189.00 | View and Order |
| pRP[Exp]-EF1A>mCherry | $189.00 | View and Order |
| pRP[Exp]-Puro-EF1A>mCherry | $189.00 | View and Order |
| pRP[Exp]-EGFP-EF1A>mCherry | $189.00 | View and Order |
| pRP[Exp]-CAG>mCherry | $189.00 | View and Order |
| pRP[Exp]-Puro-CAG>mCherry | $189.00 | View and Order |
| pRP[Exp]-EGFP-CAG>mCherry | $189.00 | View and Order |
| Lentiviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pLV[Exp]-EF1A>mCherry | $189.00 | View and Order |
| pLV[Exp]-Puro-EF1A>mCherry | $189.00 | View and Order |
| pLV[Exp]-EGFP-EF1A>mCherry | $189.00 | View and Order |
| pLV[Exp]-EGFP/Puro-EF1A>mCherry | $189.00 | View and Order |
| pLV[Exp]-CMV>mCherry | $189.00 | View and Order |
| pLV[Exp]-Puro-CMV>mCherry | $189.00 | View and Order |
| pLV[Exp]-TRE>mCherry | $189.00 | View and Order |
| pLV[Exp]-Puro-TRE>mCherry | $189.00 | View and Order |
| AAV Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAAV[Exp]-CAG>mCherry | $189.00 | View and Order |
| pAAV[Exp]-CAG>mCherry:WPRE | $189.00 | View and Order |
| pAAV[Exp]-CMV>mCherry | $189.00 | View and Order |
| pAAV[Exp]-CMV>mCherry:WPRE | $189.00 | View and Order |
| pAAV[Exp]-SYN1>mCherry | $189.00 | View and Order |
| pAAV[Exp]-SYN1>mCherry:WPRE | $189.00 | View and Order |
| Adenoviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAV[Exp]-CMV>mCherry | $189.00 | View and Order |
| pAd5/F35[Exp]-CMV>mCherry | $189.00 | View and Order |
| Transposon Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pPB[Exp]-EF1A>mCherry | $189.00 | View and Order |
| pPB[Exp]-Puro-EF1A>mCherry | $189.00 | View and Order |
| pPB[Exp]-CAG>mCherry | $189.00 | View and Order |
| pPB[Exp]-Puro-CAG>mCherry | $189.00 | View and Order |
| pPB[Exp]-CMV>mCherry | $189.00 | View and Order |
| pPB[Exp]-Puro-CMV>mCherry | $189.00 | View and Order |
| Regular Plasmid Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pRP[Exp]-CMV>TagBFP2 | $189.00 | View and Order |
| pRP[Exp]-Puro-CMV>TagBFP2 | $189.00 | View and Order |
| pRP[Exp]-EF1A>TagBFP2 | $189.00 | View and Order |
| pRP[Exp]-Puro-EF1A>TagBFP2 | $189.00 | View and Order |
| pRP[Exp]-CAG>TagBFP2 | $189.00 | View and Order |
| pRP[Exp]-Puro-CAG>TagBFP2 | $189.00 | View and Order |
| Lentiviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pLV[Exp]-EF1A>TagBFP2 | $189.00 | View and Order |
| pLV[Exp]-Puro-EF1A>TagBFP2 | $189.00 | View and Order |
| pLV[Exp]-CMV>TagBFP2 | $189.00 | View and Order |
| pLV[Exp]-Puro-CMV>TagBFP | $189.00 | View and Order |
| AAV Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAAV[Exp]-CAG>TagBFP2 | $189.00 | View and Order |
| pAAV[Exp]-CAG>TagBFP2:WPRE | $189.00 | View and Order |
| pAAV[Exp]-CMV>TagBFP2 | $189.00 | View and Order |
| pAAV[Exp]-CMV>TagBFP2:WPRE | $189.00 | View and Order |
| pAAV[Exp]-SYN1>TagBFP2 | $189.00 | View and Order |
| pAAV[Exp]-SYN1>TagBFP2:WPRE | $189.00 | View and Order |
| Transposon Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pPB[Exp]-EF1A>TagBFP2 | $189.00 | View and Order |
| pPB[Exp]-Puro-EF1A>TagBFP2 | $189.00 | View and Order |
| pPB[Exp]-CAG>TagBFP2 | $189.00 | View and Order |
| pPB[Exp]-Puro-CAG>TagBFP2 | $189.00 | View and Order |
| pPB[Exp]-CMV>TagBFP2 | $189.00 | View and Order |
| pPB[Exp]-Puro-CMV>TagBFP2 | $189.00 | View and Order |
Scramble shRNA
As a negative control for RNAi experiments, we offer scramble shRNA control plasmids encoding shRNA that does not recognize any sequence in the human or mouse genome.
Regular plasmid
Lentiviral
AAV
Adenoviral
Transposon
| Regular Plasmid Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pRP[shRNA]-U6>Scramble_shRNA | $149.00 | View and Order |
| pRP[shRNA]-Puro-U6>Scramble_shRNA | $149.00 | View and Order |
| pRP[shRNA]-Hygro-U6>Scramble_shRNA | $149.00 | View and Order |
| pRP[shRNA]-EGFP-U6>Scramble_shRNA | $149.00 | View and Order |
| pRP[shRNA]-EGFP/Puro-U6>Scramble_shRNA | $149.00 | View and Order |
| pRP[shRNA]-mCherry-U6>Scramble_shRNA | $149.00 | View and Order |
| pRP[shRNA]-mCherry/Puro-U6>Scramble_shRNA | $149.00 | View and Order |
| pRP[shRNA]-mCherry/Neo-U6>Scramble_shRNA | $149.00 | View and Order |
| pRP[miR30]-EGFP/Puro-EF1A>mCherry:Scramble_miR30-shRNA | $299.00 | View and Order |
| Lentiviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pLV[shRNA]-Puro-U6>Scramble_shRNA | $149.00 | View and Order |
| pLV[shRNA]-Neo-U6>Scramble_shRNA | $149.00 | View and Order |
| pLV[shRNA]-Hygro-U6>Scramble_shRNA | $149.00 | View and Order |
| pLV[shRNA]-EGFP-U6>Scramble_shRNA | $149.00 | View and Order |
| pLV[shRNA]-EGFP/Puro-U6>Scramble_shRNA | $149.00 | View and Order |
| pLV[shRNA]-EGFP/Neo-U6>Scramble_shRNA | $149.00 | View and Order |
| pLV[shRNA]-mCherry-U6>Scramble_shRNA | $149.00 | View and Order |
| pLV[shRNA]-mCherry/Puro-U6>Scramble_shRNA | $149.00 | View and Order |
| pLV[shRNA]-mCherry/Bsd-U6>Scramble_shRNA | $149.00 | View and Order |
| pLV[shRNA]-LacI/Puro-U6/2xLacO>Scramble_shRNA | $149.00 | View and Order |
| AAV Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAAV[shRNA]-CAG>EGFP-U6>Scramble_shRNA | $149.00 | View and Order |
| pAAV[shRNA]-CAG>mCherry-U6>Scramble_shRNA | $149.00 | View and Order |
| pscAAV[shRNA]-EGFP-U6>Scramble_shRNA | $149.00 | View and Order |
| pscAAV[shRNA]-mCherry-U6>Scramble_shRNA | $149.00 | View and Order |
| pAAV[miR30]-CAG>EGFP:Scramble_miR30-shRNA:WPRE | $299.00 | View and Order |
| pAAV[miR30]-CAG>mCherry:Scramble_miR30-shRNA:WPRE | $299.00 | View and Order |
| pAAV[miR30]-CMV>EGFP:Scramble_miR30-shRNA:WPRE | $299.00 | View and Order |
| Adenoviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAV[shRNA]-EGFP-U6>Scramble_shRNA | $299.00 | View and Order |
| pAV[shRNA]-mCherry-U6>Scramble_shRNA | $299.00 | View and Order |
| pAV[shRNA]-TagBFP2-U6>Scramble_shRNA | $299.00 | View and Order |
| pAV[miR30]-CMV>EGFP:Scramble_miR30-shRNA | $299.00 | View and Order |
| Transposon Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pPB[shRNA]-Puro-U6>Scramble_shRNA | $149.00 | View and Order |
| pPB[shRNA]-Bsd-U6>Scramble_shRNA | $149.00 | View and Order |
| pPB[shRNA]-EGFP-U6>Scramble_shRNA | $149.00 | View and Order |
| pPB[shRNA]-EGFP/Puro-U6>Scramble_shRNA | $149.00 | View and Order |
| pPB[shRNA]-EGFP/Bsd-U6>Scramble_shRNA | $149.00 | View and Order |
| pPB[shRNA]-mCherry-U6>Scramble_shRNA | $149.00 | View and Order |
| pPB[shRNA]-mCherry/Puro-U6>Scramble_shRNA | $149.00 | View and Order |
| pPB[shRNA]-mCherry/Bsd-U6>Scramble_shRNA | $149.00 | View and Order |
Non-Targeting gRNA
As a negative control for CRISPR experiments, we offer non-targeting/scramble gRNA plasmids encoding gRNA and dual expression vectors encoding gRNA and Cas9. Our control gRNAs do not recognize any sequence in the human or mouse genome.
Regular plasmid
Lentiviral
AAV
Adenoviral
Transposon
| Regular Plasmid Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pRP[gRNA]-EGFP-U6>Scramble_gRNA | $149.00 | View and Order |
| pRP[gRNA]-EGFP/Puro-U6>Scramble_gRNA | $149.00 | View and Order |
| pRP[CRISPR]-EGFP-hCas9-U6>Scramble_gRNA | $149.00 | View and Order |
| pRP[CRISPR]-EGFP/Puro-hCas9-U6>Scramble_gRNA | $149.00 | View and Order |
| pRP[2gRNA]-EGFP-U6>Scramble_gRNA1-U6>Scramble_gRNA2 | $329.00 | View and Order |
| pRP[2gRNA]-EGFP/Puro-U6>Scramble_gRNA1-U6>Scramble_gRNA2 | $329.00 | View and Order |
| pRP[2CRISPR]-EGFP-hCas9-U6>Scramble_gRNA1-U6>Scramble_gRNA2 | $329.00 | View and Order |
| pRP[2CRISPR]-EGFP/Puro-hCas9-U6>Scramble_gRNA1-U6>Scramble_gRNA2 | $329.00 | View and Order |
| pRP[gRNA]-mCherry/Puro-U6>Scramble_gRNA | $149.00 | View and Order |
| pRP[CRISPR]-mCherry-hCas9-U6>Scramble_gRNA | $149.00 | View and Order |
| pRP[CRISPR]-mCherry/Puro-hCas9-U6>Scramble_gRNA | $149.00 | View and Order |
| Lentiviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pLV[gRNA]-Puro-U6>Scramble_gRNA | $149.00 | View and Order |
| pLV[gRNA]-EGFP-U6>Scramble_gRNA | $149.00 | View and Order |
| pLV[gRNA]-EGFP/Puro-U6>Scramble_gRNA | $149.00 | View and Order |
| pLV[2gRNA]-EGFP-U6>Scramble_gRNA1-macU6>Scramble_gRNA2 | $329.00 | View and Order |
| pLV[2gRNA]-EGFP/Puro-U6>Scramble_gRNA1-macU6>Scramble_gRNA2 | $329.00 | View and Order |
| pLV[CRISPR]-hCas9/Puro-U6>Scramble_gRNA | $149.00 | View and Order |
| pLV[2CRISPR]-hCas9/Puro-U6>Scramble_gRNA1-macU6>Scramble_gRNA2 | $329.00 | View and Order |
| pLV[gRNA]-mCherry-U6>Scramble_gRNA | $149.00 | View and Order |
| pLV[gRNA]-mCherry/Puro-U6>Scramble_gRNA | $149.00 | View and Order |
| pLV[msgRNA]-Puro-U6>Scramble_msgRNA | $149.00 | View and Order |
| AAV Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAAV[SagRNA]-CAG>EGFP-U6>Scramble_SagRNA | $149.00 | View and Order |
| pAAV[SagRNA]-CAG>EGFP/Puro-U6>Scramble_SagRNA | $149.00 | View and Order |
| pAAV[CRISPR]-SaCas9-U6>Scramble_SagRNA | $149.00 | View and Order |
| pAAV[msSagRNA]-MS2/P65/HSF1-U6>Scramble_msSagRNA | $189.00 | View and Order |
| pAAV[gRNA]-CAG>EGFP-U6>Scramble_gRNA | $149.00 | View and Order |
| pAAV[gRNA]-CAG>EGFP/Puro-U6>Scramble_gRNA | $149.00 | View and Order |
| Adenoviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAV[gRNA]-EGFP-U6>Scramble_gRNA | $299.00 | View and Order |
| pAV[CRISPR]-hCas9-U6>Scramble_gRNA | $299.00 | View and Order |
| pAV[CRISPR]-hCas9/EGFP-U6>Scramble_gRNA | $299.00 | View and Order |
| Transposon Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pPB[gRNA]-EGFP-U6>Scramble_gRNA | $149.00 | View and Order |
| pPB[gRNA]-EGFP/Puro-U6>Scramble_gRNA | $149.00 | View and Order |
| pPB[CRISPR]-EGFP-hCas9-U6>Scramble_gRNA | $149.00 | View and Order |
| pPB[CRISPR]-EGFP/Puro-hCas9-U6>Scramble_gRNA | $149.00 | View and Order |
| pPB[gRNA]-mCherry-U6>Scramble_gRNA | $149.00 | View and Order |
| pPB[gRNA]-mCherry/Puro-U6>Scramble_gRNA | $149.00 | View and Order |
| pPB[CRISPR]-mCherry-hCas9-U6>Scramble_gRNA | $149.00 | View and Order |
| pPB[CRISPR]-mCherry/Puro-hCas9-U6>Scramble_gRNA | $149.00 | View and Order |
Negative Controls
As a side-by-side negative control for gene overexpression experiments, we offer ORF stuffer expression vectors encoding amino acids 2-83 of E. coli beta-galactosidase.
Regular plasmid
Lentiviral
AAV
Adenoviral
Transposon
| Regular Plasmid Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pRP[Exp]-CMV>ORF_Stuffer | $189.00 | View and Order |
| pRP[Exp]-Puro-CMV>ORF_Stuffer | $189.00 | View and Order |
| pRP[Exp]-EGFP-CMV>ORF_Stuffer | $189.00 | View and Order |
| pRP[Exp]-EGFP/Puro-CMV>ORF_Stuffer | $189.00 | View and Order |
| pRP[Exp]-mCherry-CMV>ORF_Stuffer | $189.00 | View and Order |
| pRP[Exp]-mCherry/Puro-CMV>ORF_Stuffer | $189.00 | View and Order |
| pRP[Exp]-CAG>ORF_Stuffer | $189.00 | View and Order |
| pRP[Exp]-EGFP-CAG>ORF_Stuffer | $189.00 | View and Order |
| pRP[Exp]-EGFP/Puro-CAG>ORF_Stuffer | $189.00 | View and Order |
| pRP[Exp]-mCherry-CAG>ORF_Stuffer | $189.00 | View and Order |
| pRP[Exp]-mCherry/Puro-CAG>ORF_Stuffer | $189.00 | View and Order |
| Lentiviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pLV[Exp]-EF1A>ORF_Stuffer | $189.00 | View and Order |
| pLV[Exp]-Puro-EF1A>ORF_Stuffer | $189.00 | View and Order |
| pLV[Exp]-EGFP-EF1A>ORF_Stuffer | $189.00 | View and Order |
| pLV[Exp]-EGFP/Puro-EF1A>ORF_Stuffer | $189.00 | View and Order |
| pLV[Exp]-mCherry-EF1A>ORF_Stuffer | $189.00 | View and Order |
| pLV[Exp]-mCherry/Puro-EF1A>ORF_Stuffer | $189.00 | View and Order |
| pLV[Exp]-mCherry/Neo-EF1A>ORF_Stuffer | $189.00 | View and Order |
| pLV[Exp]-CMV>ORF_Stuffer | $189.00 | View and Order |
| pLV[Exp]-Puro-CMV>ORF_Stuffer | $189.00 | View and Order |
| AAV Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAAV[Exp]-CAG>ORF_Stuffer | $189.00 | View and Order |
| pAAV[Exp]-CAG>ORF_Stuffer:WPRE | $189.00 | View and Order |
| pAAV[Exp]-CMV>ORF_Stuffer | $189.00 | View and Order |
| pAAV[Exp]-CMV>ORF_Stuffer:WPRE | $189.00 | View and Order |
| Adenoviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAV[Exp]-CMV>ORF_Stuffer | $189.00 | View and Order |
| pAV[Exp]-EGFP-CMV>ORF_Stuffer | $189.00 | View and Order |
| pAV[Exp]-mCherry-CMV>ORF_Stuffer | $189.00 | View and Order |
| pAV[Exp]-EF1A>ORF_Stuffer | $189.00 | View and Order |
| pAV[Exp]-EGFP-EF1A>ORF_Stuffer | $189.00 | View and Order |
| pAV[Exp]-mCherry-EF1A>ORF_Stuffer | $189.00 | View and Order |
| Transposon Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pPB[Exp]-CAG>ORF_Stuffer | $189.00 | View and Order |
| pPB[Exp]-Puro-CAG>ORF_Stuffer | $189.00 | View and Order |
| pPB[Exp]-EGFP/Puro-CAG>ORF_Stuffer | $189.00 | View and Order |
| pPB[Exp]-mCherry/Puro-CAG>ORF_Stuffer | $189.00 | View and Order |
| pPB[Exp]-EF1A>ORF_Stuffer | $189.00 | View and Order |
| pPB[Exp]-Puro-EF1A>ORF_Stuffer | $189.00 | View and Order |
| pPB[Exp]-EGFP/Puro-EF1A>ORF_Stuffer | $189.00 | View and Order |
| pPB[Exp]-mCherry/Puro-EF1A>ORF_Stuffer | $189.00 | View and Order |
For many more options, search our popular vectors below:
Search for RefSeq genes (e.g. RHO)
Resources
Featured Citations
Trb3 Controls Mesenchymal Stem Cell Lineage Fate and Enhances Bone Regeneration by Scaffold-Mediated Local Gene Delivery
Jiabing Fan, et al.
Biomaterials, 2020, doi: 10.1016/j.biomaterials.2020.120445
Products used:
- Adeno-Associated Virus (AAV) Packaging (AAV1, 2, 5, 6, 8 & 9)
- Lentivirus Packaging
- Vector Cloning
Abstract: Aberrant lineage commitment of mesenchymal stem cells (MSCs) in marrow contributes to abnormal bone formation due to reduced osteogenic and increased adipogenic potency. While several major transcriptional factors associated with lineage differentiation have been found during the last few decades, the molecular switch for MSC fate determination and its role in skeletal regeneration remains largely unknown, limiting creation of effective therapeutic approaches. Tribbles homolog 3 (Trb3), a member of tribbles family pseudokinases, is known to exert diverse roles in cellular differentiation. Here, we investigated the reciprocal role of Trb3 in the regulation of osteogenic and adipogenic differentiation of MSCs in the context of bone formation, and examined the mechanisms by which Trb3 controls the adipo-osteogenic balance. Trb3 promoted osteoblastic commitment of MSCs at the expense of adipocyte differentiation. Mechanistically, Trb3 regulated cell-fate choice of MSCs through BMP/Smad and Wnt/β-catenin signals. Importantly, in vivo local delivery of Trb3 using a novel gelatin-conjugated caffeic acid-coated apatite/PLGA (GelCA-PLGA) scaffold stimulated robust bone regeneration and inhibited fat-filled cyst formation in rodent non-healing mandibular defect models. These findings demonstrate Trb3-based therapeutic strategies that favor osteoblastogenesis over adipogenesis for improved skeletal regeneration and future treatment of bone-loss disease. The distinctive approach implementing a scaffold-mediated local gene transfer may further broaden the translational use of targeting specific therapeutic gene related to lineage commitment for clinical bone treatment.
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Educational Resources
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Shine bright, little cell: A comprehensive guide to choosing the right reporter gene
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