Premade CRISPR Vectors
VectorBuilder offers solutions to streamline your CRISPR experiments, including a wide selection of premade CRISPR vectors encoding Cas proteins and non-targeting gRNA in viral and non-viral backbones.
Highlights
Ready-to-ship premade CRISPR vectors available in viral and non-viral backbones.
Functionally validated vector backbones and components with 100% sequence guarantee.
Add down stream services, including plasmid DNA preparation and virus packaging in just a few clicks.Cas Proteins
We offer a selection of vectors for the efficient expression of Cas proteins, covering a range of applications, including conventional CRISPR for knockout or knockin (Cas9), CRISPRa (dCas9 + transcriptional activators), CRISPRi (dCas9 + transcriptional repressors), and Tet-inducible Cas9 for tight control over expression. For many more Cas protein and construct options please use our popular vectors search function or view our full selection of popular ORFs
Cas9
dCas9 for CRISPRa
dCas9 for CRISPRi
Tet-inducible Cas9
 Regular Plasmid Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pRP[Exp]-CMV>hCas9 | $189 | View and Order |
| pRP[Exp]-Puro-CMV>hCas9 | $189 | View and Order |
| pRP[Exp]-Hygro-CMV>hCas9 | $189 | View and Order |
| pRP[Exp]-Bsd-CMV>hCas9 | $189 | View and Order |
| pRP[Exp]-EGFP-CMV>hCas9 | $189 | View and Order |
| pRP[Exp]-CBh>hCas9 | $189 | View and Order |
| pRP[Exp]-Puro-CBh>hCas9 | $189 | View and Order |
| pRP[Exp]-Hygro-CBh>hCas9 | $189 | View and Order |
| pRP[Exp]-Bsd-CBh>hCas9 | $189 | View and Order |
| pRP[Exp]-EGFP-CBh>hCas9 | $189 | View and Order |
 Lentiviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pLV[Exp]-Puro-EF1A>hCas9 | $189 | View and Order |
| pLV[Exp]-Bsd-EF1A>hCas9 | $189 | View and Order |
| pLV[Exp]-Puro-CMV>hCas9 | $189 | View and Order |
| pLV[Exp]-Bsd-CMV>hCas9 | $189 | View and Order |
| pLV[Exp]-Puro-TRE>hCas9 | $189 | View and Order |
| pLV[Exp]-Bsd-TRE>hCas9 | $189 | View and Order |
 AAV Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAAV[Exp]-CMV>SaCas9 | $189 | View and Order |
| pAAV[Exp]-SYN1>SaCas9 | $189 | View and Order |
 Adenoviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAV[Exp]-CMV>hCas9 | $189 | View and Order |
| pAV[Exp]-EGFP-CMV>hCas9 | $189 | View and Order |
 Transposon Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pPB[Exp]-Puro-EF1A>hCas9 | $189 | View and Order |
| pPB[Exp]-Hygro-EF1A>hCas9 | $189 | View and Order |
| pPB[Exp]-EGFP-EF1A>hCas9 | $189 | View and Order |
| pPB[Exp]-EGFP/Puro-EF1A>hCas9 | $189 | View and Order |
| pPB[Exp]-EGFP/Hygro-EF1A>hCas9 | $189 | View and Order |
| pPB[Exp]-Puro-CAG>hCas9 | $189 | View and Order |
| pPB[Exp]-Hygro-CAG>hCas9 | $189 | View and Order |
| pPB[Exp]-EGFP-CAG>hCas9 | $189 | View and Order |
| pPB[Exp]-EGFP/Puro-CAG>hCas9 | $189 | View and Order |
| pPB[Exp]-EGFP/Hygro-CAG>hCas9 | $189 | View and Order |
| Regular Plasmid Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pRP[Exp]-CMV>dCas9/VPR | $189 | View and Order |
| pRP[Exp]-Puro-CMV>dCas9/VPR | $189 | View and Order |
| pRP[Exp]-EGFP-CMV>dCas9/VPR | $189 | View and Order |
| pRP[Exp]-CMV>dCas9/VP64 | $189 | View and Order |
| pRP[Exp]-Puro-CMV>dCas9/VP64 | $189 | View and Order |
| pRP[Exp]-EGFP-CMV>dCas9/VP64 | $189 | View and Order |
| pRP[Exp]-CMV>MS2/P65/HSF1 | $189 | View and Order |
| pRP[Exp]-Puro-CMV>MS2/P65/HSF1 | $189 | View and Order |
| pRP[Exp]-EGFP-CMV>MS2/P65/HSF1 | $189 | View and Order |
| Lentiviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pLV[Exp]-Bsd-EF1A>dCas9/VPR | $189 | View and Order |
| pLV[Exp]-Puro-EF1A>dCas9/VPR | $189 | View and Order |
| pLV[Exp]-Bsd-EF1A>dCas9/VP64 | $189 | View and Order |
| pLV[Exp]-EGFP-EF1A>dCas9/VP64 | $189 | View and Order |
| pLV[Exp]-EGFP-EF1A>MS2/P65/HSF1 | $189 | View and Order |
| pLV[Exp]-Bsd-EF1A>MS2/P65/HSF1 | $189 | View and Order |
| pLV[Exp]-Puro-EF1A>MS2/P65/HSF1 | $189 | View and Order |
| AAV Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAAV[Exp]-CMV>dSaCas9/VP64 | $189 | View and Order |
| pAAV[Exp]-CMV>MS2/P65/HSF1 | $189 | View and Order |
| Transposon Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pPB[Exp]-Hygro-EF1A>dCas9/VPR | $189 | View and Order |
| pPB[Exp]-Bsd-EF1A>dCas9/VPR | $189 | View and Order |
| pPB[Exp]-EGFP-EF1A>dCas9/VPR | $189 | View and Order |
| pPB[Exp]-Hygro-EF1A>dCas9/VP64 | $189 | View and Order |
| pPB[Exp]-Bsd-EF1A>dCas9/VP64 | $189 | View and Order |
| pPB[Exp]-EGFP-EF1A>dCas9/VP64 | $189 | View and Order |
| pPB[Exp]-Hygro-EF1A>MS2/P65/HSF1 | $189 | View and Order |
| pPB[Exp]-Bsd-EF1A>MS2/P65/HSF1 | $189 | View and Order |
| Regular Plasmid Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pRP[Exp]-CMV>dCas9/KRAB | $189 | View and Order |
| pRP[Exp]-Puro-CMV>dCas9/KRAB | $189 | View and Order |
| pRP[Exp]-Hygro-CMV>dCas9/KRAB | $189 | View and Order |
| pRP[Exp]-EGFP-CMV>dCas9/KRAB | $189 | View and Order |
| pRP[Exp]-CMV>dCas9/KRAB/MeCP2 | $189 | View and Order |
| pRP[Exp]-Puro-CMV>dCas9/KRAB/MeCP2 | $189 | View and Order |
| pRP[Exp]-Hygro-CMV>dCas9/KRAB/MeCP2 | $189 | View and Order |
| pRP[Exp]-EGFP-CMV>dCas9/KRAB/MeCP2 | $189 | View and Order |
| Lentiviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pLV[Exp]-Puro-EF1A>dCas9/KRAB | $189 | View and Order |
| pLV[Exp]-Bsd-EF1A>dCas9/KRAB | $189 | View and Order |
| pLV[Exp]-Puro-EF1A>dCas9/KRAB/MeCP2 | $189 | View and Order |
| pLV[Exp]-Bsd-EF1A>dCas9/KRAB/MeCP2 | $189 | View and Order |
| pLV[Exp]-CBh>dCas9/KRAB/MeCP2/Hygro | $189 | View and Order |
| pLV[Exp]-Bsd-CBh>dCas9/KRAB/MeCP2 | $189 | View and Order |
| Transposon Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pPB[Exp]-Puro-EF1A>dCas9/KRAB | $189 | View and Order |
| pPB[Exp]-Hygro-EF1A>dCas9/KRAB | $189 | View and Order |
| pPB[Exp]-Bsd-EF1A>dCas9/KRAB | $189 | View and Order |
| pPB[Exp]-EGFP-EF1A>dCas9/KRAB | $189 | View and Order |
| pPB[Exp]-Puro-EF1A>dCas9/KRAB/MeCP2 | $189 | View and Order |
| pPB[Exp]-Hygro-EF1A>dCas9/KRAB/MeCP2 | $189 | View and Order |
| pPB[Exp]-Bsd-EF1A>dCas9/KRAB/MeCP2 | $189 | View and Order |
| pPB[Exp]-EGFP-EF1A>dCas9/KRAB/MeCP2 | $189 | View and Order |
| Regular Plasmid Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pRP[Exp]-Neo-TRE>hCas9 | $189 | View and Order |
| pRP[Exp]-Puro-TRE>hCas9 | $189 | View and Order |
| pRP[Exp]-Hygro-TRE>hCas9 | $189 | View and Order |
| pRP[Exp]-Bsd-TRE>hCas9 | $189 | View and Order |
| pRP[Exp]-EGFP-TRE>hCas9 | $189 | View and Order |
| pRP[Exp]-EGFP/Neo-TRE>hCas9 | $189 | View and Order |
| pRP[Exp]-EGFP/Puro-TRE>hCas9 | $189 | View and Order |
| pRP[Exp]-EGFP/Hygro-TRE>hCas9 | $189 | View and Order |
| pRP[Exp]-EGFP/Bsd-TRE>hCas9 | $189 | View and Order |
| Lentiviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pLV[Exp]-Neo-TRE>hCas9 | $189 | View and Order |
| pLV[Exp]-Puro-TRE>hCas9 | $189 | View and Order |
| pLV[Exp]-Hygro-TRE>hCas9 | $189 | View and Order |
| pLV[Exp]-Bsd-TRE>hCas9 | $189 | View and Order |
| AAV Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAAV[Exp]-TRE>SaCas9 | $189 | View and Order |
| pAAV[Exp]-TRE>SaCas9:WPRE | $189 | View and Order |
| Transposon Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pPB[Exp]-Neo-TRE>hCas9 | $189 | View and Order |
| pPB[Exp]-Puro-TRE>hCas9 | $189 | View and Order |
| pPB[Exp]-Hygro-TRE>hCas9 | $189 | View and Order |
| pPB[Exp]-Bsd-TRE>hCas9 | $189 | View and Order |
| pPB[Exp]-mCherry-TRE>hCas9 | $189 | View and Order |
| pPB[Exp]-EGFP-TRE>hCas9 | $189 | View and Order |
| pPB[Exp]-EGFP/Neo-TRE>hCas9 | $189 | View and Order |
| pPB[Exp]-EGFP/Puro-TRE>hCas9 | $189 | View and Order |
| pPB[Exp]-EGFP/Hygro-TRE>hCas9 | $189 | View and Order |
| pPB[Exp]-EGFP/Bsd-TRE>hCas9 | $189 | View and Order |
Non-Targeting gRNA
As a negative control for CRISPR experiments, we offer vectors encoding non-targeting gRNAs, which do not recognize any sequence in the human or mouse genome. We also offer dual expression vectors encoding non-targeting gRNA and Cas9.
| Regular Plasmid Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pRP[gRNA]-EGFP-U6>Scramble_gRNA | $149 | View and Order |
| pRP[gRNA]-EGFP/Puro-U6>Scramble_gRNA | $149 | View and Order |
| pRP[CRISPR]-EGFP-hCas9-U6>Scramble_gRNA | $149 | View and Order |
| pRP[CRISPR]-EGFP/Puro-hCas9-U6>Scramble_gRNA | $149 | View and Order |
| pRP[2gRNA]-EGFP-U6>Scramble_gRNA1-U6>Scramble_gRNA2 | $329 | View and Order |
| pRP[2gRNA]-EGFP/Puro-U6>Scramble_gRNA1-U6>Scramble_gRNA2 | $329 | View and Order |
| pRP[2CRISPR]-EGFP-hCas9-U6>Scramble_gRNA1-U6>Scramble_gRNA2 | $329 | View and Order |
| pRP[2CRISPR]-EGFP/Puro-hCas9-U6>Scramble_gRNA1-U6>Scramble_gRNA2 | $329 | View and Order |
| pRP[gRNA]-mCherry/Puro-U6>Scramble_gRNA | $149 | View and Order |
| pRP[CRISPR]-mCherry-hCas9-U6>Scramble_gRNA | $149 | View and Order |
| pRP[CRISPR]-mCherry/Puro-hCas9-U6>Scramble_gRNA | $149 | View and Order |
| Lentiviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pLV[gRNA]-Puro-U6>Scramble_gRNA | $149 | View and Order |
| pLV[gRNA]-EGFP-U6>Scramble_gRNA | $149 | View and Order |
| pLV[gRNA]-EGFP/Puro-U6>Scramble_gRNA | $149 | View and Order |
| pLV[2gRNA]-EGFP-U6>Scramble_gRNA1-macU6>Scramble_gRNA2 | $329 | View and Order |
| pLV[2gRNA]-EGFP/Puro-U6>Scramble_gRNA1-macU6>Scramble_gRNA2 | $329 | View and Order |
| pLV[CRISPR]-hCas9/Puro-U6>Scramble_gRNA | $149 | View and Order |
| pLV[2CRISPR]-hCas9/Puro-U6>Scramble_gRNA1-macU6>Scramble_gRNA2 | $329 | View and Order |
| pLV[gRNA]-mCherry-U6>Scramble_gRNA | $149 | View and Order |
| pLV[gRNA]-mCherry/Puro-U6>Scramble_gRNA | $149 | View and Order |
| pLV[msgRNA]-Puro-U6>Scramble_msgRNA | $149 | View and Order |
| AAV Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAAV[SagRNA]-CAG>EGFP-U6>Scramble_SagRNA | $149 | View and Order |
| pAAV[SagRNA]-CAG>EGFP/Puro-U6>Scramble_SagRNA | $149 | View and Order |
| pAAV[SaCRISPR]-SaCas9-U6>Scramble_SagRNA | $149 | View and Order |
| pAAV[msSagRNA]-MS2/P65/HSF1-U6>Scramble_SagRNA | $189 | View and Order |
| pAAV[gRNA]-CAG>EGFP-U6>Scramble_gRNA | $149 | View and Order |
| pAAV[gRNA]-CAG>EGFP/Puro-U6>Scramble_gRNA | $149 | View and Order |
| Adenoviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAV[gRNA]-EGFP-U6>Scramble_gRNA | $299 | View and Order |
| pAV[CRISPR]-hCas9-U6>Scramble_gRNA | $299 | View and Order |
| pAV[CRISPR]-hCas9/EGFP-U6>Scramble_gRNA | $299 | View and Order |
| Transposon Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pPB[gRNA]-EGFP-U6>Scramble_gRNA | $149 | View and Order |
| pPB[gRNA]-EGFP/Puro-U6>Scramble_gRNA | $149 | View and Order |
| pPB[CRISPR]-EGFP-hCas9-U6>Scramble_gRNA | $149 | View and Order |
| pPB[CRISPR]-EGFP/Puro-hCas9-U6>Scramble_gRNA | $149 | View and Order |
| pPB[gRNA]-mCherry-U6>Scramble_gRNA | $149 | View and Order |
| pPB[gRNA]-mCherry/Puro-U6>Scramble_gRNA | $149 | View and Order |
| pPB[CRISPR]-mCherry-hCas9-U6>Scramble_gRNA | $149 | View and Order |
| pPB[CRISPR]-mCherry/Puro-hCas9-U6>Scramble_gRNA | $149 | View and Order |
For many more options, search our popular vectors below:
Search for RefSeq genes (e.g. RHO)
Resources
Featured Citations
Genetic and pharmacological modulation of DNA mismatch repair heterogeneous tumors promotes immune surveillance
Vito Amodio, et al.
Cancer Cell, 2023, doi: 10.1016/j.ccell.2022.12.003
Products used:
Abstract: Patients affected by colorectal cancer (CRC) with DNA mismatch repair deficiency (MMRd), often respond to immune checkpoint blockade therapies, while those with mismatch repair-proficient (MMRp) tumors generally do not. Interestingly, a subset of MMRp CRCs contains variable fractions of MMRd cells, but it is unknown how their presence impacts immune surveillance. We asked whether modulation of the MMRd fraction in MMR heterogeneous tumors acts as an endogenous cancer vaccine by promoting immune surveillance. To test this hypothesis, we use isogenic MMRp (Mlh1+/+) and MMRd (Mlh1-/-) mouse CRC cells. MMRp/MMRd cells mixed at different ratios are injected in immunocompetent mice and tumor rejection is observed when at least 50% of cells are MMRd. To enrich the MMRd fraction, MMRp/MMRd tumors are treated with 6-thioguanine, which leads to tumor rejection. These results suggest that genetic and pharmacological modulation of the DNA mismatch repair machinery potentiate the immunogenicity of MMR heterogeneous tumors.
>See more<Collapse 
Inhibition of glycogen synthase kinase-3 enhances NRF2 protein stability, nuclear localisation and target gene transcription in pancreatic beta cells
Patibandla, et al.
Redox Biology, 2024, doi: 10.1016/j.redox.2024.103117
Products used:
- Vector Cloning ( Mammalian CRISPR Lentiviral Vector )
- Vector Cloning ( Mammalian Gene Expression Adenoviral Vector )
- Lentivirus Packaging
- Adenovirus Packaging
Abstract: Accumulation of reactive oxygen species (i.e., oxidative stress) is a leading cause of beta cell dysfunction and apoptosis in diabetes. NRF2 (NF-E2 p45-related factor-2) regulates the adaptation to oxidative stress, and its activity is negatively regulated by the redox-sensitive CUL3 (cullin-3) ubiquitin ligase substrate adaptor KEAP1 (Kelch-like ECH-associated protein-1). Additionally, NRF2 is repressed by the insulin-regulated Glycogen Synthase Kinase-3 (GSK3). We have demonstrated that phosphorylation of NRF2 by GSK3 enhances β-TrCP (beta-transducin repeat-containing protein) binding and ubiquitylation by CUL1 (cullin-1), resulting in increased proteasomal degradation of NRF2. Thus, we hypothesise that inhibition of GSK3 activity or β-TrCP binding upregulates NRF2 and so protects beta cells against oxidative stress. We have found that treating the pancreatic beta cell line INS-1 832/13 with the KEAP1 inhibitor TBE31 significantly enhanced NRF2 protein levels. The presence of the GSK3 inhibitor CT99021 or the β-TrCP-NRF2 protein-protein interaction inhibitor PHAR, along with TBE31, resulted in prolonged NRF2 stability and enhanced nuclear localisation (P < 0.05). TBE31-mediated induction of NRF2-target genes encoding NAD(P)H quinone oxidoreductase 1 (Nqo1), glutamate-cysteine ligase modifier (Gclm) subunit and heme oxygenase (Hmox1) was significantly enhanced by the presence of CT99021 or PHAR (P < 0.05) in both INS-1 832/13 and in isolated mouse islets. Identical results were obtained using structurally distinct GSK3 inhibitors and inhibition of KEAP1 with sulforaphane. In summary, we demonstrate that GSK3 and β-TrCP/CUL1 regulate the proteasomal degradation of NRF2, enhancing the impact of KEAP1 regulation, and so contributes to the redox status of pancreatic beta cells. Inhibition of GSK3, or β-TrCP/CUL1 binding to NRF2 may represent a strategy to protect beta cells from oxidative stress.
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Educational Resources
Vector GuidesRead more about our CRISPR gene editing vectors and CRISPR gene regulation vectors in our guides.
WebpagesLearn more about CRISPR and VectorBuilder’s custom-made CRISPR solutions, from viruses for single-gene knockout to whole-genome knockout libraries, and more: CRISPR Genome Editing Solutions.
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