Premade Tet System Vectors
At VectorBuilder, we offer a range of premade vectors encoding Tet regulatory proteins designed to minimize leaky expression, ensuring potent and rapid expression (Tet-On) or repression (Tet-Off) of your gene of interest following induction. We also offer Tet-inducible control vectors and Cas9 to further streamline your inducible gene expression experiments and enhance research efficiency.
Highlights
Ready-to-ship premade Tet system vectors available in viral and non-viral backbones.
Functionally validated vector backbones and components with 100% sequence guarantee.
Add downstream services, including plasmid DNA preparation and virus packaging in just a few clicks.Tet-On Regulators (tTS/rtTA)
We offer premade vectors for the efficient expression of Tet regulatory proteins for use in the Tet-On system, available in viral and non-viral vector backbones.
 Regular Plasmid Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pRP[Exp]-Puro-CMV>tTS/rtTA | $189 | View and Order |
| pRP[Exp]-Hygro-CMV>tTS/rtTA | $189 | View and Order |
| pRP[Exp]-Bsd-CMV>tTS/rtTA | $189 | View and Order |
| pRP[Exp]-Neo-CMV>tTS/rtTA | $189 | View and Order |
| pRP[Exp]-Puro-EF1A>tTS/rtTA | $189 | View and Order |
| pRP[Exp]-Hygro-EF1A>tTS/rtTA | $189 | View and Order |
| pRP[Exp]-Neo-EF1A>tTS/rtTA | $189 | View and Order |
| pRP[Exp]-Bsd-EF1A>tTS/rtTA | $189 | View and Order |
 Lentiviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pLV[Exp]-Puro-EF1A>tTS/rtTA | $189 | View and Order |
| pLV[Exp]-Hygro-EF1A>tTS/rtTA | $189 | View and Order |
| pLV[Exp]-Bsd-EF1A>tTS/rtTA | $189 | View and Order |
| pLV[Exp]-Neo-EF1A>tTS/rtTA | $189 | View and Order |
| pLV[Exp]-Puro-CMV>tTS/rtTA | $189 | View and Order |
| pLV[Exp]-Hygro-CMV>tTS/rtTA | $189 | View and Order |
| pLV[Exp]-Bsd-CMV>tTS/rtTA | $189 | View and Order |
| pLV[Exp]-Neo-CMV>tTS/rtTA | $189 | View and Order |
 AAV Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAAV[Exp]-CAG>tTS/rtTA | $189 | View and Order |
| pAAV[Exp]-CAG>tTS/rtTA:WPRE | $189 | View and Order |
| pAAV[Exp]-CMV>tTS/rtTA | $189 | View and Order |
| pAAV[Exp]-CMV>tTS/rtTA:WPRE | $189 | View and Order |
 Transposon Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pPB[Exp]-Puro-EF1A>tTS/rtTA | $189 | View and Order |
| pPB[Exp]-Hygro-EF1A>tTS/rtTA | $189 | View and Order |
| pPB[Exp]-Bsd-EF1A>tTS/rtTA | $189 | View and Order |
| pPB[Exp]-Neo-EF1A>tTS/rtTA | $189 | View and Order |
| pPB[Exp]-Puro-CAG>tTS/rtTA | $189 | View and Order |
| pPB[Exp]-Hygro-CAG>tTS/rtTA | $189 | View and Order |
| pPB[Exp]-Bsd-CAG>tTS/rtTA | $189 | View and Order |
| pPB[Exp]-Neo-CAG>tTS/rtTA | $189 | View and Order |
Tet-Off Regulator (tTA2)
We offer premade vectors for the efficient expression of Tet regulatory proteins for use in the Tet-Off system, available in viral and non-viral vector backbones.
| Regular Plasmid Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pRP[Exp]-CMV>tTA2 | $189 | View and Order |
| pRP[Exp]-Puro-CMV>tTA2 | $189 | View and Order |
| pRP[Exp]-Hygro-CMV>tTA2 | $189 | View and Order |
| pRP[Exp]-Bsd-CMV>tTA2 | $189 | View and Order |
| pRP[Exp]-Neo-CMV>tTA2 | $189 | View and Order |
| pRP[Exp]-EF1A>tTA2 | $189 | View and Order |
| pRP[Exp]-Puro-EF1A>tTA2 | $189 | View and Order |
| pRP[Exp]-Hygro-EF1A>tTA2 | $189 | View and Order |
| pRP[Exp]-Bsd-EF1A>tTA2 | $189 | View and Order |
| pRP[Exp]-Neo-EF1A>tTA2 | $189 | View and Order |
| Lentiviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pLV[Exp]-EF1A>tTA2 | $189 | View and Order |
| pLV[Exp]-Puro-CMV>tTA2 | $189 | View and Order |
| pLV[Exp]-Hygro-EF1A>tTA2 | $189 | View and Order |
| pLV[Exp]-Bsd-EF1A>tTA2 | $189 | View and Order |
| pLV[Exp]-CMV>tTA2 | $189 | View and Order |
| pLV[Exp]-Neo-CMV>tTA2 | $189 | View and Order |
| pLV[Exp]-Hygro-CMV>tTA2 | $189 | View and Order |
| pLV[Exp]-Bsd-CMV>tTA2 | $189 | View and Order |
| pLV[Exp]-Neo-CMV>tTA2 | $189 | View and Order |
| AAV Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAAV[Exp]-CAG>tTA2 | $189 | View and Order |
| pAAV[Exp]-CAG>tTA2:WPRE | $189 | View and Order |
| pAAV[Exp]-CMV>tTA2 | $189 | View and Order |
| pAAV[Exp]-CMV>tTA2:WPRE | $189 | View and Order |
| Transposon Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pPB[Exp]-Puro-EF1A>tTA2 | $189 | View and Order |
| pPB[Exp]-Hygro-EF1A>tTA2 | $189 | View and Order |
| pPB[Exp]-Bsd-EF1A>tTA2 | $189 | View and Order |
| pPB[Exp]-Neo-EF1A>tTA2 | $189 | View and Order |
| pPB[Exp]-Puro-CMV>tTA2 | $189 | View and Order |
| pPB[Exp]-Hygro-CMV>tTA2 | $189 | View and Order |
| pPB[Exp]-Bsd-CMV>tTA2 | $189 | View and Order |
| pPB[Exp]-Neo-CMV>tTA2 | $189 | View and Order |
Tet-inducible Controls
Popular Tet vectors
VectorBuilder offers a panel of popular Tet vectors in both all-in-one and dual vector formats. Plasmid DNA preparation and virus packaging can be purchased as downstream services when adding popular Tet vectors into shopping cart.
You can use the vector pickers below for ordering popular Tet vectors.
Selection tips
- TRE driven GOI expression vector and Tet regulatory protein expression vector are NOT all-in-one Tet vectors. To achieve Tet regulated gene expression, you need the expression of both your GOI driven by TRE promoter and Tet regulatory protein in your cells of interest.
- Select a Tet regulatory protein expression vector for generating stable cell lines expressing tTA, tTS and/or rtTA.
All-in-one (Tet-on)
Note: If you need lentiviral all-in-one (Tet-on) vectors, please send us a design request.
TRE driven GOI expression
Tet regulatory protein expression
If you unable to find your desired Tet vectors using the vector pickers above, simply send us a design request describing your needs and our scientists will assist you from there.
Premade Tet-inducible GFP and mCherry control vectors
VectorBuilder also offers premade vectors encoding GFP or mCherry under the control of a TRE promoter as a control for Tet-regulated gene expression experiments.
As a control for Tet-regulated gene expression, we offer premade vectors encoding GFP or mCherry under the control of a TRE promoter.
Tet-inducible GFP
Tet-inducible mCherry
| Regular Plasmid Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pRP[Exp]-TRE>EGFP | $189 | View and Order |
| pRP[Exp]-Puro-TRE>EGFP | $189 | View and Order |
| pRP[Exp]-Neo-TRE>EGFP | $189 | View and Order |
| pRP[TetOn]-TRE>EGFP-rev(CBh>tTS/rtTA) | $239 | View and Order |
| pRP[TetOn]-Puro-TRE>EGFP-rev(CBh>tTS/rtTA) | $239 | View and Order |
| pRP[TetOn]-Neo-TRE>EGFP-rev(CBh>tTS/rtTA) | $239 | View and Order |
| pRP[TetOn]-TRE>EGFP-rev(CMV>tTS/rtTA) | $239 | View and Order |
| Lentiviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pLV[Exp]-TRE>EGFP | $189 | View and Order |
| pLV[Exp]-Puro-TRE>EGFP | $189 | View and Order |
| pLV[Exp]-Hygro-TRE>EGFP | $189 | View and Order |
| pLV[Exp]-Neo-TRE>EGFP | $189 | View and Order |
| pLV[Exp]-Bsd-TRE>EGFP | $189 | View and Order |
| AAV Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAAV[Exp]-TRE>EGFP | $189 | View and Order |
| pAAV[Exp]-TRE>EGFP:WPRE | $189 | View and Order |
| pAAV[TetOn]-TRE>EGFP-rev(CBh>tTS/rtTA) | $239 | View and Order |
| Transposon Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pPB[Exp]-Puro-TRE>EGFP | $189 | View and Order |
| pPB[Exp]-Hygro-TRE>EGFP | $189 | View and Order |
| pPB[Exp]-Neo-TRE>EGFP | $189 | View and Order |
| pPB[Exp]-Bsd-TRE>EGFP | $189 | View and Order |
| pPB[TetOn]-TRE>EGFP-rev(CBh>tTS/rtTA) | $239 | View and Order |
| pPB[TetOn]-Puro-TRE>EGFP-rev(CBh>tTS/rtTA) | $239 | View and Order |
| pPB[TetOn]-Hygro-TRE>EGFP-rev(CBh>tTS/rtTA) | $239 | View and Order |
| pPB[TetOn]-Neo-TRE>EGFP-rev(CBh>tTS/rtTA) | $239 | View and Order |
| pPB[TetOn]-Bsd-TRE>EGFP-rev(CBh>tTS/rtTA) | $239 | View and Order |
| Regular Plasmid Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pRP[Exp]-TRE>mCherry | $189 | View and Order |
| pRP[Exp]-Puro-TRE>mCherry | $189 | View and Order |
| pRP[Exp]-Neo-TRE>mCherry | $189 | View and Order |
| pRP[TetOn]-TRE>mCherry-rev(CBh>tTS/rtTA) | $239 | View and Order |
| pRP[TetOn]-Neo-TRE>mCherry-rev(CBh>tTS/rtTA) | $239 | View and Order |
| pRP[TetOn]-Puro-TRE>mCherry-rev(CBh>tTS/rtTA) | $239 | View and Order |
| Lentiviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pLV[Exp]-TRE>mCherry | $189 | View and Order |
| pLV[Exp]-Puro-TRE>mCherry | $189 | View and Order |
| pLV[Exp]-Neo-TRE>mCherry | $189 | View and Order |
| AAV Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAAV[Exp]-TRE>mCherry | $189 | View and Order |
| pAAV[Exp]-TRE>mCherry:WPRE | $189 | View and Order |
| pAAV[TetOn]-TRE>mCherry-rev(CBh>tTS/rtTA) | $239 | View and Order |
| Transposon Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pPB[Exp]-TRE>mCherry | $189 | View and Order |
| pPB[Exp]-Puro-TRE>mCherry | $189 | View and Order |
| pPB[Exp]-Hygro-TRE>mCherry | $189 | View and Order |
| pPB[Exp]-Neo-TRE>mCherry | $189 | View and Order |
| pPB[Exp]-Bsd-TRE>mCherry | $189 | View and Order |
| pPB[TetOn]-TRE>mCherry-rev(CBh>tTS/rtTA) | $239 | View and Order |
| pPB[TetOn]-Puro-TRE>mCherry-rev(CBh>tTS/rtTA) | $239 | View and Order |
| pPB[TetOn]-Neo-TRE>mCherry-rev(CBh>tTS/rtTA) | $239 | View and Order |
| pPB[TetOn]-Hygro-TRE>mCherry-rev(CBh>tTS/rtTA) | $239 | View and Order |
| pPB[TetOn]-Bsd-TRE>mCherry-rev(CBh>tTS/rtTA) | $239 | View and Order |
Tet-inducible Cas9
For tight control over your CRISPR experiments, we offer vectors encoding Tet-inducible Cas9.
| Regular Plasmid Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pRP[Exp]-Neo-TRE>hCas9 | $189 | View and Order |
| pRP[Exp]-Puro-TRE>hCas9 | $189 | View and Order |
| pRP[Exp]-Hygro-TRE>hCas9 | $189 | View and Order |
| pRP[Exp]-Bsd-TRE>hCas9 | $189 | View and Order |
| pRP[Exp]-EGFP-TRE>hCas9 | $189 | View and Order |
| pRP[Exp]-EGFP/Neo-TRE>hCas9 | $189 | View and Order |
| pRP[Exp]-EGFP/Puro-TRE>hCas9 | $189 | View and Order |
| pRP[Exp]-EGFP/Hygro-TRE>hCas9 | $189 | View and Order |
| pRP[Exp]-EGFP/Bsd-TRE>hCas9 | $189 | View and Order |
| Lentiviral Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pLV[Exp]-Neo-TRE>hCas9 | $189 | View and Order |
| pLV[Exp]-Puro-TRE>hCas9 | $189 | View and Order |
| pLV[Exp]-Hygro-TRE>hCas9 | $189 | View and Order |
| pLV[Exp]-Bsd-TRE>hCas9 | $189 | View and Order |
| AAV Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pAAV[Exp]-TRE>SaCas9 | $189 | View and Order |
| pAAV[Exp]-TRE>SaCas9:WPRE | $189 | View and Order |
| Transposon Vector Name |
Price (USD) | Buy Now |
|---|---|---|
| pPB[Exp]-Neo-TRE>hCas9 | $189 | View and Order |
| pPB[Exp]-Puro-TRE>hCas9 | $189 | View and Order |
| pPB[Exp]-Hygro-TRE>hCas9 | $189 | View and Order |
| pPB[Exp]-Bsd-TRE>hCas9 | $189 | View and Order |
| pPB[Exp]-mCherry-TRE>hCas9 | $189 | View and Order |
| pPB[Exp]-EGFP-TRE>hCas9 | $189 | View and Order |
| pPB[Exp]-EGFP/Neo-TRE>hCas9 | $189 | View and Order |
| pPB[Exp]-EGFP/Puro-TRE>hCas9 | $189 | View and Order |
| pPB[Exp]-EGFP/Hygro-TRE>hCas9 | $189 | View and Order |
| pPB[Exp]-EGFP/Bsd-TRE>hCas9 | $189 | View and Order |
For many more options, search our popular vectors below:
Search for RefSeq genes (e.g. RHO)
Resources
Educational Resources
Vector Guides WebpagesLearn more about the different Tet systems available at VectorBuilder by clicking here: Inducible gene expression solutions.
Featured Citations
H3K36 methylation maintains cell identity by regulating opposing lineage programmes
Hoetker, et al.
Nature Cell Biology, 2023, doi: 10.1038/s41556-023-01191-z
Products used:
- Vector Cloning (Mammalian Tet-inducible Gene Expression Lentiviral Vector)
- Vector Cloning (Mammalian shRNA Knockdown Lentiviral Vector)
- shRNA Gene Knockdown Solutions
- Lentivirus Packaging
Abstract: The epigenetic mechanisms that maintain differentiated cell states remain incompletely understood. Here, we employed histone mutants to uncover a crucial role for H3K36-methylation in the maintenance of cell identities across diverse developmental contexts. Focusing on the experimental induction of pluripotency, we show that H3K36M-mediated depletion of H3K36-methylation endows fibroblasts with a plastic state poised to acquire pluripotency in nearly all cells. At a cellular level, H3K36M facilitates epithelial plasticity by rendering fibroblasts insensitive to TGFb signals. At a molecular level, H3K36M enables the decommissioning of mesenchymal enhancers and the parallel activation of epithelial/stem cell enhancers. This enhancer rewiring is Tet-dependent and redirects Sox2 from promiscuous somatic to pluripotency targets. Our findings reveal a previously unappreciated dual role for H3K36-methylation in the maintenance of cell identity by integrating a crucial developmental pathway into sustained expression of cell type-specific programs, and by opposing the expression of alternative lineage programs through enhancer methylation.
>See more <Collapse 
Concurrent inhibition of oncogenic and wild-type RAS-GTP for cancer therapy
Holderfield, et al.
Nature, 2024, doi: 10.1038/s41586-024-07205-6
Products used:
- Vector Cloning (Mammalian Tet-inducible Gene Expression Lentiviral Vector)
- Vector Cloning (Tet Regulatory Protein Expression Lentiviral Vector))
- Lentivirus Packaging
Abstract: RAS oncogenes (collectively NRAS, HRAS and especially KRAS) are among the most frequently mutated genes in cancer, with common driver mutations occurring at codons 12, 13 and 61. Small molecule inhibitors of the KRAS(G12C) oncoprotein have demonstrated clinical efficacy in patients with multiple cancer types and have led to regulatory approvals for the treatment of non-small cell lung cancer. Nevertheless, KRASG12C mutations account for only around 15% of KRAS-mutated cancers, and there are no approved KRAS inhibitors for the majority of patients with tumours containing other common KRAS mutations. Here we describe RMC-7977, a reversible, tri-complex RAS inhibitor with broad-spectrum activity for the active state of both mutant and wild-type KRAS, NRAS and HRAS variants (a RAS(ON) multi-selective inhibitor). Preclinically, RMC-7977 demonstrated potent activity against RAS-addicted tumours carrying various RAS genotypes, particularly against cancer models with KRAS codon 12 mutations (KRASG12X). Treatment with RMC-7977 led to tumour regression and was well tolerated in diverse RAS-addicted preclinical cancer models. Additionally, RMC-7977 inhibited the growth of KRASG12X cancer models that are resistant to KRAS(G12C) inhibitors owing to restoration of RAS pathway signalling. Thus, RAS(ON) multi-selective inhibitors can target multiple oncogenic and wild-type RAS isoforms and have the potential to treat a wide range of RAS-addicted cancers with high unmet clinical need. A related RAS(ON) multi-selective inhibitor, RMC-6236, is currently under clinical evaluation in patients with KRAS-mutant solid tumours (ClinicalTrials.gov identifier: NCT05379985).
>See more <Collapse