Premade Transposon System Vectors
At VectorBuilder, we offer high-quality premade vectors for your transposon-based experiments, available for piggyBac, Sleeping Beauty, and Tol2 systems. Pair our premade transposon and transposase expression vectors for an efficient non-viral solution for permanent integration into the host genome.
Highlights
Ready-to-ship premade transposon system vectors (piggyBac, Sleeping Beauty, and Tol2).
Functionally validated vector backbones and components with 100% sequence guarantee.
Add downstream services, including plasmid DNA preparation in just a few clicks.PiggyBac
We offer piggyBac control vectors for a variety of applications, including overexpression, RNAi, and CRISPR. These vectors are designed to work with our piggyBac transposase expression vectors for high-efficiency gene delivery.
Gene expression controls
RNAi controls
CRISPR controls
Transposase
| Vector Name | Price (USD) | Buy Now |
|---|---|---|
| pPB[Exp]-EF1A>EGFP | $189.00 | View and Order |
| pPB[Exp]-EF1A>ORF_Stuffer | $189.00 | View and Order |
| pPB[Exp]-Puro-EF1A>ORF_Stuffer | $189.00 | View and Order |
| pPB[Exp]-Puro-EF1A>EGFP | $189.00 | View and Order |
| pPB[Exp]-CMV>EGFP | $189.00 | View and Order |
| pPB[Exp]-Puro-CMV>ORF_Stuffer | $189.00 | View and Order |
| pPB[Exp]-Puro-CMV>EGFP | $189.00 | View and Order |
| pPB[Exp]-CAG>EGFP | $189.00 | View and Order |
| pPB[TetOn]-TRE>EGFP-rev(CAG>tTS/rtTA) | $189.00 | View and Order |
| Vector Name | Price (USD) | Buy Now |
|---|---|---|
| pPB[shRNA]-Puro-U6>Scramble_shRNA | $149.00 | View and Order |
| pPB[shRNA]-Bsd-U6>Scramble_shRNA | $149.00 | View and Order |
| pPB[shRNA]-EGFP-U6>Scramble_shRNA | $149.00 | View and Order |
| pPB[shRNA]-EGFP/Puro-U6>Scramble_shRNA | $149.00 | View and Order |
| pPB[shRNA]-EGFP/Bsd-U6>Scramble_shRNA | $149.00 | View and Order |
| pPB[shRNA]-mCherry-U6>Scramble_shRNA | $149.00 | View and Order |
| pPB[shRNA]-mCherry/Puro-U6>Scramble_shRNA | $149.00 | View and Order |
| pPB[shRNA]-mCherry/Bsd-U6>Scramble_shRNA | $149.00 | View and Order |
| Vector Name | Price (USD) | Buy Now |
|---|---|---|
| pPB[gRNA]-EGFP-U6>Scramble_gRNA | $149.00 | View and Order |
| pPB[gRNA]-mCherry-U6>Scramble_gRNA | $149.00 | View and Order |
| pPB[gRNA]-EGFP/Puro-U6>Scramble_gRNA | $149.00 | View and Order |
| pPB[gRNA]-mCherry/Puro-U6>Scramble_gRNA | $149.00 | View and Order |
| pPB[CRISPR]-EGFP-hCas9-U6>Scramble_gRNA | $149.00 | View and Order |
| pPB[CRISPR]-mCherry-hCas9-U6>Scramble_gRNA | $149.00 | View and Order |
| pPB[CRISPR]-EGFP/Puro-hCas9-U6>Scramble_gRNA | $149.00 | View and Order |
| pPB[CRISPR]-mCherry/Puro-hCas9-U6>Scramble_gRNA | $149.00 | View and Order |
| Vector Name | Price (USD) | Buy Now |
|---|---|---|
| pRP[Exp]-CMV>PBase | $189.00 | View and Order |
| pRP[Exp]-CAG>PBase | $189.00 | View and Order |
| pRP[Exp]-EF1A>PBase | $189.00 | View and Order |
Sleeping Beauty
We offer premade Sleeping Beauty vectors as controls for gene expression, designed to work with Sleeping Beauty transposase expression vectors for high-efficiency gene delivery.
| Vector Name | Price (USD) | Buy Now |
|---|---|---|
| pSB[Exp]-CMV>EGFP | $189.00 | View and Order |
| pSB[Exp]-CMV>ORF_Stuffer | $189.00 | View and Order |
| pSB[Exp]-Puro-CMV>ORF_Stuffer | $189.00 | View and Order |
| pSB[Exp]-Puro-CMV>EGFP | $189.00 | View and Order |
| pSB[Exp]-CMV>EGFP/Puro | $189.00 | View and Order |
| pSB[Exp]-CAG>EGFP | $189.00 | View and Order |
| pSB[Exp]-EF1A>ORF_Stuffer | $189.00 | View and Order |
| pSB[Exp]-CMV>EGFP | $189.00 | View and Order |
| pSB[Exp]-CAG>EGFP | $189.00 | View and Order |
Tol2
We offer premade Tol2 vectors as controls for gene expression, designed to work with our Tol2 transposase expression vectors for high-efficiency gene delivery.
Gene expression controls
Transposase
| Vector Name | Price (USD) | Buy Now |
|---|---|---|
| pTol2[Exp]-CMV>EGFP | $189.00 | View and Order |
| pTol2[Exp]-CMV>ORF_Stuffer | $189.00 | View and Order |
| pTol2[Exp]-Puro-CMV>ORF_Stuffer | $189.00 | View and Order |
| pTol2[Exp]-Puro-CMV>EGFP | $189.00 | View and Order |
| pTol2[Exp]-CAG>EGFP | $189.00 | View and Order |
| pTol2[Exp]-CAG>ORF_Stuffer | $189.00 | View and Order |
| pTol2[Exp]-Puro-CAG>ORF_Stuffer | $189.00 | View and Order |
| pTol2[Exp]-Puro-CAG>EGFP | $189.00 | View and Order |
| Vector Name | Price (USD) | Buy Now |
|---|---|---|
| pRP[Exp]-CMV>Tol2 | $189.00 | View and Order |
| pRP[Exp]-CAG>Tol2 | $189.00 | View and Order |
| pRP[Exp]-EF1A>Tol2 | $189.00 | View and Order |
For many more options, search our popular vectors below:
Search for RefSeq genes (e.g. RHO)
Resources
Featured Citations
Using piggyBac transposon gene expression vectors to transfect Zscan5b gene into mouse pluripotent stem cells
Yamada, et al.
Cell Press STAR Protocols, 2021, doi: 10.1016/j.xpro.2021.100811
Products used:
- PiggyBac Vector Cloning
Abstract: We recently demonstrated that the zygotic gene Zscan5b is involved in maintaining the genomic integrity of pluripotent stem cells (PSCs) during mitosis and that Zscan5b gene expression can prevent chromosomal abnormalities. Here, we provide a detailed protocol for the transfection of mouse embryonic stem cells (mESCs) and mouse-induced PSCs (miPSCs) with a Zscan5b constitutive expression plasmid, using the piggyBac transposon gene expression vector system. For complete details on the use and execution of this protocol, please refer to Ogawa et al. (2019).
>See more<Collapse 
Targeted delivery of a PD-1-blocking scFv by CD133-specific CAR-T cells using nonviral Sleeping Beauty transposition shows enhanced antitumour efficacy for advanced hepatocellular carcinoma
Methods: CD133-specific CAR-T cells secreting PD-1 blocking scFv (CD133 CAR-T and PD-1 s cells) were constructed using a sleeping beauty transposon system from minicircle technology, and the antitumour efficacy of CD133 CAR-T and PD-1 s cells was analysed in vitro and in vivo.
Results: A univariate analysis showed that CD133 expression in male patients at the late stage (II and III) was significantly associated with worse progression-free survival (PFS) (P = 0.0057) and overall survival (OS) (P = 0.015), and a multivariate analysis showed a trend toward worse OS (P = 0.041). Male patients with advanced HCC exhibited an approximately 20-fold higher PD-L1 combined positive score (CPS) compared with those with HCC at an early stage. We successfully generated CD133 CAR-T and PD-1 s cells that could secrete PD-1 blocking scFv based on a sleeping beauty system involving minicircle vectors. CD133 CAR-T and PD-1 s cells exhibited significant antitumour activity against HCC in vitro and in xenograft mouse models. Thus, CD133 CAR-T and PD-1 s cells may be a therapeutically tractable strategy for targeting CD133-positive CSCs in male patients with advanced HCC.
Conclusions: Our study provides a nonviral strategy for constructing CAR-T cells that could also secrete checkpoint blockade inhibitors based on a Sleeping Beauty system from minicircle vectors and revealed a potential benefit of this strategy for male patients with advanced HCC and high CD133 expression (median immunohistochemistry score > 2.284).
Keywords: CAR-T; CD133; Hepatocellular carcinoma; Minicircle; PD-1; Sleeping Beauty system.
>See more<Collapse
Yang, et al.
BMC Medicine, 2023, doi: 10.1186/s12916-023-03016-0
Products used:
- Sleeping Beauty Vector Cloning
Abstract:
Background: CD133 is considered a marker for cancer stem cells (CSCs) in several types of tumours, including hepatocellular carcinoma (HCC). Chimeric antigen receptor-specific T (CAR-T) cells targeting CD133-positive CSCs have emerged as a tool for the clinical treatment of HCC, but immunogenicity, the high cost of clinical-grade recombinant viral vectors and potential insertional mutagenesis limit their clinical application.
Methods: CD133-specific CAR-T cells secreting PD-1 blocking scFv (CD133 CAR-T and PD-1 s cells) were constructed using a sleeping beauty transposon system from minicircle technology, and the antitumour efficacy of CD133 CAR-T and PD-1 s cells was analysed in vitro and in vivo.
Results: A univariate analysis showed that CD133 expression in male patients at the late stage (II and III) was significantly associated with worse progression-free survival (PFS) (P = 0.0057) and overall survival (OS) (P = 0.015), and a multivariate analysis showed a trend toward worse OS (P = 0.041). Male patients with advanced HCC exhibited an approximately 20-fold higher PD-L1 combined positive score (CPS) compared with those with HCC at an early stage. We successfully generated CD133 CAR-T and PD-1 s cells that could secrete PD-1 blocking scFv based on a sleeping beauty system involving minicircle vectors. CD133 CAR-T and PD-1 s cells exhibited significant antitumour activity against HCC in vitro and in xenograft mouse models. Thus, CD133 CAR-T and PD-1 s cells may be a therapeutically tractable strategy for targeting CD133-positive CSCs in male patients with advanced HCC.
Conclusions: Our study provides a nonviral strategy for constructing CAR-T cells that could also secrete checkpoint blockade inhibitors based on a Sleeping Beauty system from minicircle vectors and revealed a potential benefit of this strategy for male patients with advanced HCC and high CD133 expression (median immunohistochemistry score > 2.284).
Keywords: CAR-T; CD133; Hepatocellular carcinoma; Minicircle; PD-1; Sleeping Beauty system.
Serotonergic modulation of vigilance states in zebrafish and mice
Zhao, et al.
Nature Communications, 2024, doi: 10.1038/s41467-024-47021-0
Products used:
Abstract: Vigilance refers to being alertly watchful or paying sustained attention to avoid potential threats. Animals in vigilance states reduce locomotion and have an enhanced sensitivity to aversive stimuli so as to react quickly to dangers.Here we report that an unconventional 5-HT driven mechanism operating at neural circuit level which shapes the internal state underlying vigilance behavior in zebrafish and male mice. The neural signature of internal vigilance state was characterized by persistent low-frequency high-amplitude neuronal synchrony in zebrafish dorsal pallium and mice prefrontal cortex. The neuronal synchronization underlying vigilance was dependent on intense release of 5-HT induced by persistent activation of either DRN 5-HT neuron or local 5-HT axon terminals in related brain regions via activation of 5-HTR7. Thus, we identify a mechanism of vigilance behavior across species that illustrates the interplay between neuromodulators and neural circuits necessary to shape behavior states.
>See more<Collapse 
Educational Resources
Vector GuidesRead more about our transposon systems in our guides.
Blog posts