Premade Control Viruses
VectorBuilder provides experiment-ready control viruses spanning a wide range of viral systems and applications, including popular viral vector constructs for lentivirus, AAV, adenovirus, MMLV, MSCV, and VSV. Offered in a range of scales, these viruses cover both in vitro and in vivo experiments, ensuring that you can select the right control for your experiment.
Highlights
High quality, high titer viruses
- Experimentally validated
- Stringent QC including titer and sterility measurement
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- Multiple scales available
- Variety of fluorescent and antibiotic markers to choose from
Made by the experts
- Decades of experience in vector design, cloning, and packaging
- Optimized vectors and packaging processes
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Featured Citations
Trb3 Controls Mesenchymal Stem Cell Lineage Fate and Enhances Bone Regeneration by Scaffold-Mediated Local Gene Delivery
Jiabing Fan, et al.
Biomaterials, 2020, doi: 10.1016/j.biomaterials.2020.120445
Products used:
- Adeno-Associated Virus (AAV) Packaging (AAV1, 2, 5, 6, 8 & 9)
- Lentivirus Packaging
- Vector Cloning
Abstract: Aberrant lineage commitment of mesenchymal stem cells (MSCs) in marrow contributes to abnormal bone formation due to reduced osteogenic and increased adipogenic potency. While several major transcriptional factors associated with lineage differentiation have been found during the last few decades, the molecular switch for MSC fate determination and its role in skeletal regeneration remains largely unknown, limiting creation of effective therapeutic approaches. Tribbles homolog 3 (Trb3), a member of tribbles family pseudokinases, is known to exert diverse roles in cellular differentiation. Here, we investigated the reciprocal role of Trb3 in the regulation of osteogenic and adipogenic differentiation of MSCs in the context of bone formation, and examined the mechanisms by which Trb3 controls the adipo-osteogenic balance. Trb3 promoted osteoblastic commitment of MSCs at the expense of adipocyte differentiation. Mechanistically, Trb3 regulated cell-fate choice of MSCs through BMP/Smad and Wnt/β-catenin signals. Importantly, in vivo local delivery of Trb3 using a novel gelatin-conjugated caffeic acid-coated apatite/PLGA (GelCA-PLGA) scaffold stimulated robust bone regeneration and inhibited fat-filled cyst formation in rodent non-healing mandibular defect models. These findings demonstrate Trb3-based therapeutic strategies that favor osteoblastogenesis over adipogenesis for improved skeletal regeneration and future treatment of bone-loss disease. The distinctive approach implementing a scaffold-mediated local gene transfer may further broaden the translational use of targeting specific therapeutic gene related to lineage commitment for clinical bone treatment.
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FAQ
For in vitro, we recommend starting with smaller scales (mini or pilot) to initially test the virus in your experimental system, prior to scaling up to medium/large scales for in vitro experiments and ultra-purified scales for both in vitro and in vivo experiments. Please refer to the table below for details on how the virus has been purified for each of our viral systems.
| Scale | Application | Lentivirus | AAV | Adenovirus | MMLV | MSCV | VSV |
|---|---|---|---|---|---|---|---|
| Mini | Cell culture | PEG concentrated | PEG concentrated | Centrifugation | PEG concentrated | PEG concentrated | PEG concentrated |
| Pilot | |||||||
| Medium | |||||||
| Large | Ultrafritration | ||||||
| Ultra-purified medium | Cell culture and in vivo | Sucrose gradient | CsCl gradient | CsCl gradient | Sucrose gradient | Sucrose gradient | Sucrose gradient |
| Ultra-purified large |