Learning Center
Community
-
Brochures & Flyers -
Upcoming Events -
Technical Bulletin -
Testimonials -
Citations
Components used in a vector (e.g. promoters & ORFs) are automatically assigned names in most cases. These component names are then automatically assembled to produce the final vector name. Rules for assigning names are described below.
Rules for assigning names to vector components (e.g. promoters, ORFs or linkers)
Components from VectorBuilder's popular component collections: The component's common name is used.
Example: CMV, EGFP, Neo
ORFs retrieved from VectorBuilder's gene-based databases: ORF name is composed of gene symbol followed by bracketed RefSeq accession (or gene ID for species that don't have RefSeq annotation). A species abbreviation in lower case is added in front of the gene symbol following the rules below:
Sequences pasted by user: User must define the name of their pasted sequence in order to add the sequence to the vector. User defined name will be shown in brackets. Please note that if the user pasted a sequence that has 100% sequence identity to a component in VectorBuilder’s databases, the component name will be automatically set to the official name of the VectorBuilder component.
Example of a user pasted component: {MyGene}
ORF from VectorBuilder’s database that is edited by user: When an ORF retrieved from VectorBuilder’s ORF database has been edited by the user to introduce mutations, a * will be automatically added next to the official ORF name to indicate that the ORF sequence is mutated. The user can then choose to use this name or change it to a different name. If the user chooses to change the name, brackets will be added to indicate that the name is defined by the user. Please note that epitope tags added to an ORF through the Add Tag function are not considered as mutations.
Example of an ORF from VectorBuilder’s database that is edited by user: hRHO[NM_000539.3]* for automatically assigned name, which can be changed by the user to another name such as {MyGene}
Rules for allowing users to change automatically assigned names
In order to ensure consistency, users cannot change automatically assigned component names under most circumstances. But there are a few exceptions as listed below:
Rules for assigning vector names
Rules for assembling vector name as illustrated by an example
Vector name: pLV[Exp]-Hygro-CMV>FLAG/hRHO[NM_000539.3]*/10xHis:IRES:3xNLS/EGFP/HA
Interpretations:
LV: Vector backbone is lentivirus.
Exp: Biological application is mammalian gene expression.
Hygro: Marker gene is hygromycin B resistance gene.
CMV: Promoter for driving the gene of interest is CMV.
FLAG/hRHO[NM_000539.3]*/10xHis:IRES:3xNLS/EGFP/HA: The gene of interest is a polycistron containing multiple ORFs. The first ORF is hRHO[NM_000539.3], whose sequence is modified by the user (indicated by *), and is tagged with FLAG at the N terminus and 10xHis at the C terminus. It is followed by IRES, and then followed by the second ORF, which is EGFP tagged with 3xNLS at the N terminus and HA at the C terminus.