Testimonials

VectorBuilder is proud to share our client's testimonials:

  • Before using VectorBuilder, producing lentiviral overexpression vectors for our in vitro models was a recurring and time-consuming enterprise. We faced frequent challenges in every experimental step, which made it difficult to plan the actual experiments. Setting up the process with VectorBuilder was very efficient, thanks to their great expertise and support. Once we started using VectorBuilder's services, we noticed a substantial increase in productivity as well as a more uniform and predictable transduction pattern of our target cells.

    We went on to use VectorBuilder for a large-scale CRISPR screen with a custom library spanning over 1,600 genes. With the provided virus, we were able to easily maintain cell representation and we derived a number of interesting hit genes, which we are excited to follow up on. A big thank you to the entire VectorBuilder Team!

    Dominic Schmid

    University of Basel

  • We recently purchased a lentiviral plasmid for constitutive expression of GFP and luciferase and the corresponding lentivirus from VectorBuilder. We have infected several tumor cell lines with excellent results. The lentiviral stocks worked very nicely, saving us a lot of time. We are now using these modified cell lines for monitoring tumor growth in animal models using in vivo imaging.

    Javier Alonso

    Research institute for Rare Diseases, Spain

  • I would like to share with you my positive experience purchasing a lentiviral vector from VectorBuilder.
     
    First, I am an immunologist and have little experience with cloning. In my recent project, we needed to overexpress a gene in melanoma cells. The transfection efficiency using plasmids in this cell line was extremely low, less than 10%. Then, we tried to use antibiotics to select transfected cells and establish stably expressing cell lines. Unfortunately, for unknown reasons, the ‘stable transfectants’ lost the transfected genes! I was frustrated at this point, until I met VectorBuilder at the EACR conference in Amsterdam. Using the user-friendly website from VectorBuilder, I designed for the first time in my life my own lentiviral vector and got the product in less than one month. My group leader, who has a lot of cloning experience, could not believe that it worked so quickly. Of course, most important are the experimental results. To access the transduction efficiency, I included an EGFP tag in the vector. Now, I can transduce the melanoma cells with nearly 100% positivity. In the following assays, the genes transduced into the tumor cells resulted in a 6-fold induction in tumor immunogenicity.
    Taken together, I strongly recommend VectorBuilder to researchers, especially those who are not so experienced with cloning, and am convinced that the service and product quality from VectorBuilder can further help me to proceed with my own projects more effectively.

    Fang Zhao

    UK-Essen, Germany

  • VectorBuilder’s service makes designing vectors incredibly simple and there’s no software required, it can all be done through their easy to use website. The lentivirus production service is a massive bonus as it’s so quick and reasonably priced. There’s really no reason to make our own lentivirus anymore. Thanks for a fantastic service.

    Timothy London

    TC Biopharm, UK

  • We were searching for a way to outsource the cloning of our vectors and we are very happy with the service. The versatility of the tools make it a great time saver and the vectors were delivered on-time. Lentiviruses packaged are also very reliable and have been very successful in transduction studies in primary cells. Great customer service with fast response times and the team at VectorBuilder are very knowledgeable.

    Kathy Ngo

    Aridis Pharmaceuticals, USA

  • I have been successfully cloning for 3+ years, but the last time we tried generating KO cell lines with CRISPR/Cas9 vectors, we were not successful. This was upsetting, especially since I invested a lot of time in these efforts. Then, by chance, I attended one of VectorBuilder's seminars. Due to their reasonable prices and the fact I could focus my time on other work, we decided to give their lentiviral (dual gRNA) Cas9 plasmid a try. The turnaround time was reasonable, their customer service was excellent and they actually gave feedback about the status of my cloning projects. Their website is also easy to use. But most importantly: using their Cas9 dual gRNA vector, I was able to successfully generate KO cell lines! 

    I recommend VectorBuilder to CRISPRCas9 users without any hesitation. 

    Anna Salamero Boix

    Georg-Speyer-Haus Frankfurt, Germany

  • I’ve used VectorBuilder’s lentivirus packaging service. The virus titer was as promised. Strong fluorescence was observed after transduction. The gene knockdown efficiency was also satisfactory.

    Yunhao Liang

    Southern Medical University, China

  • I purchased a lentivirus vector and received it in less than two weeks. I am so glad that I found VectorBuilder, so I can design my lentiviral vector by picking any promotor, and from a variety of antibiotic selection markers and fluorescent tags. Upon transducing several cell lines, my target genes were highly and stably expressed with expression persisting for at least after one month after undergoing selection.

    Xianghong Chen

    H. Lee Moffitt Cancer Center & Research Institute, USA

  • VectorBuilder is an intuitive tool for developing vectors at affordable prices. I was in need of a CRISPR lentiviral system with a Puro selection cassette due to working with delicate cells that cannot handle FACS sorting pressures. With their online interface, it was easy to construct the vector I wanted and received the vector in a timely manner! It was simple to produce virus and test it in our cell culture and generated clonal cells with good gene knockdown. I definitely will be ordering more vectors from VectorBuilder to finish up my thesis!

    Christophe Langouet-Astrie

    University of Colorado, USA

  • I’ve used VectorBuilder’s vector construction and lentivirus packaging services. The turnaround time was short and everything worked well. Also, I received quick responses from the customer support team.

    Xijuan Chen

    Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, China

  • I used the lentivirus packaging service from VectorBuilder, and the process is very easy. All I needed to do is to ship a small quantity of transfer plasmid, and VectorBuilder will do the rest. Then I received the high titer virus at the end.

    Xueqing Xia

    UT MD Anderson Cancer Center, USA

  • We have used VectorBuilder for multiple vectors including regular, lentiviral, Tet-inducible, and CRISPR/Cas9 vectors. Designing the vectors is super easy and we have had good luck with them. I would definitely recommend their products!

    Steven Wall

    Texas A&M University, USA

  • VectorBuilder did vector construction and lentivirus packaging for my shRNA knockdown experiment. The transduction efficiency of the lentivirus was high. Their staff were very considerate, and related technical questions were addressed quickly. I’ve finished many experiments and I’m preparing the manuscript for submission.

    Mengyuan Fan

    School of Nature Conservation, Beijing Forestry University, China

  • I have been using ready-to-use lentiviruses from Vectorbuilder and they are working great. I used them against iPSC-derived neurons, and the transduction and expression of transgenes are remarkable. The vector backbones provide multiple options to tag your GOIs with fluorescent proteins or implement antibiotic selection markers. Their vector designing tools are helpful. Customer service is reliable. Good luck!

    Utpal Das

    University of California San Diego, USA

  • VectorBuilder provides fast and economic service for plasmids construction, which saves a lot of time for me. I mostly construct lentivirus plasmids with them and so far have a positive impression for their products. They respond to my requests quickly and honestly so the communication with VectorBuilder’s staffs is one of the best parts of the service.

    Eiji Yoshihara

    Lundquist Institute at Harbor-UCLA Medical Center, USA

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