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Eureka Moments at the Bench

Me First! How Do I Optimize a Vector with Multiple Genes?
Gene delivery systems allow for efficient introduction of not just one gene, but often multiple components, including a promoter, open reading frame (ORF), and marker. Read More ››
Enhance Your Culture: How to Improve Bacterial Growth for Efficient Gene Delivery
Cloning serves as the foundation for most gene delivery experiments and is often where initial challenges emerge. Read More ››
Slipping under Titer: Why Is My Viral Titer so Low?
Many gene delivery experiments utilize viral vectors for efficient delivery of genetic information to target cells. Read More ››
Bring on the EtBr: How Can I Be Sure My Gel Reflects My Sample?
Gel electrophoresis is a conventional method to assess the size, composition, and amount of your DNA. Read More ››
Setting up for Success: How Do I Ensure I Have the Right Template for PCR?
When I was a graduate student, we called the lab’s PCR machine the “Magic Box,” because the reagents that we put inside could produce exactly what we were looking for, something completely unexpected, or nothing at all. Read More ››
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