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Vector Information

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Vector Summary

Vector ID:: VB010000-9877mhm

Vector Name:

Vector Type:: Mammalian Single-gRNA Expression Lentiviral Vector

Vector Size:: 8408 bp

Viral Genome Size:: 4933 bp undersized genome oversized genome

gRNA:: AAVS1[gRNA#1]

Guide Sequence:: GGGGCCACTAGGGACAGGAT

Marker:: EGFP:T2A:Puro

Plasmid Copy Number:: High

Antibiotic Resistance:: Ampicillin

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Vector Description: None [Edit]

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Order Information How to order

Vector Cloning Service

Deliverable:
E. coli stock
Cloning Host Strain: {[ obj.cloning_host|boatFixI18n|translate ]}

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Vector Components

{[opt.name \| boatFixI18nName:'boatComponent'\|translate]}
{[opt.name \| boatFixI18nName:'boatComponent'\|translate]}
RSV promoter	1-229	229	View Details
5' LTR-ΔU3	230-410	181	View Details
misc_feature1	411-520	110	View Details
Ψ	521-565	45	View Details
misc_feature2	566-1074	509	View Details
RRE	1075-1308	234	View Details
misc_feature3	1309-1802	494	View Details
cPPT	1803-1920	118	View Details
misc_feature4	1921-1926	6	View Details
U6 promoter	1927-2175	249	View Details
{n}	2176-2176	1	View Details
AAVS1[gRNA#1]	2177-2272	96	View Details
Terminator	2273-2278	6	View Details
misc_feature5	2279-2318	40	View Details
hPGK promoter	2319-2823	505	View Details
misc_feature8	2824-2853	30	View Details
EGFP:T2A:Puro	2854-4233	1380	View Details
misc_feature9	4234-4264	31	View Details
WPRE	4265-4862	598	View Details
misc_feature11	4863-4927	65	View Details
3' LTR-ΔU3	4928-5162	235	View Details
misc_feature10	5163-5234	72	View Details
SV40 early pA	5235-5369	135	View Details
misc_feature11	5370-6322	953	View Details
AmpiciIIin	6323-7183	861	View Details
misc_feature12	7184-7353	170	View Details
pUC ori	7354-7942	589	View Details
misc_feature13	7943-8408	466	View Details

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40ee65991af8fd647d871d00187693c1

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