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Vector Information

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Vector Summary

Vector ID:: VB010000-9911czh

Vector Name:

Vector Type:: Mammalian CRISPR Lentiviral Vector (Single gRNA) Guide

Vector Size:: 12501 bp

Viral Genome Size:: 8674 bp undersized genome oversized genome

gRNA:: Scramble[gRNA#1]

Guide Sequence:: GTGTAGTTCGACCATTCGTG

Nuclease:: hCas9:T2A:Hygro

Plasmid Copy Number:: High

Antibiotic Resistance:: Ampicillin

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Vector Description: None [Edit]

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Order Information How to order

Vector Cloning Service

Deliverable:
E. coli stock
Cloning Host Strain: {[ obj.cloning_host|boatFixI18n|translate ]}

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Vector Components

{[opt.name \| boatFixI18nName:'boatComponent'\|translate]}
{[opt.name \| boatFixI18nName:'boatComponent'\|translate]}
CMV promoter	1-581	581	View Details
5' LTR-ΔU3	582-762	181	View Details
misc_feature1	763-872	110	View Details
Ψ	873-917	45	View Details
misc_feature2	918-1426	509	View Details
RRE	1427-1660	234	View Details
misc_feature	1661-2151	491	View Details
cPPT	2152-2268	117	View Details
Undefined	2269-2314	46	View Details
U6 promoter	2315-2563	249	View Details
{n}	2564-2564	1	View Details
Scramble[gRNA#1]	2565-2660	96	View Details
Terminator	2661-2666	6	View Details
misc_feature5	2667-2706	40	View Details
EFS promoter	2707-2938	232	View Details
misc_feature8	2939-2968	30	View Details
hCas9:T2A:Hygro	2969-8326	5358	View Details
misc_feature9	8327-8357	31	View Details
WPRE	8358-8955	598	View Details
misc_feature11	8956-9020	65	View Details
3' LTR-ΔU3	9021-9255	235	View Details
misc_feature10	9256-9327	72	View Details
SV40 early pA	9328-9462	135	View Details
Undefined	9463-10415	953	View Details
AmpiciIIin	10416-11276	861	View Details
misc_feature12	11277-11446	170	View Details
pUC ori	11447-12035	589	View Details
misc_feature13	12036-12501	466	View Details

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ef7ba26e37054f6a27af4d000e149cae

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