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Vector Information

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Vector Summary

Vector ID:: VB010000-9913tyd

Vector Name:

Vector Type:: Mammalian CRISPR Lentiviral Vector (Single gRNA) Guide

Vector Size:: 11874 bp

Viral Genome Size:: 8047 bp undersized genome oversized genome

gRNA:: Scramble[gRNA#1]

Guide Sequence:: GTGTAGTTCGACCATTCGTG

Nuclease:: hCas9:T2A:Bsd

Plasmid Copy Number:: High

Antibiotic Resistance:: Ampicillin

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Vector Description: None [Edit]

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Order Information How to order

Vector Cloning Service

Deliverable:
E. coli stock
Cloning Host Strain: {[ obj.cloning_host|boatFixI18n|translate ]}

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Vector Components

{[opt.name \| boatFixI18nName:'boatComponent'\|translate]}
{[opt.name \| boatFixI18nName:'boatComponent'\|translate]}
CMV promoter	1-581	581	View Details
5' LTR-ΔU3	582-762	181	View Details
misc_feature1	763-872	110	View Details
Ψ	873-917	45	View Details
misc_feature2	918-1426	509	View Details
RRE	1427-1660	234	View Details
misc_feature	1661-2151	491	View Details
cPPT	2152-2268	117	View Details
Undefined	2269-2314	46	View Details
U6 promoter	2315-2563	249	View Details
{n}	2564-2564	1	View Details
Scramble[gRNA#1]	2565-2660	96	View Details
Terminator	2661-2666	6	View Details
misc_feature5	2667-2706	40	View Details
EFS promoter	2707-2938	232	View Details
misc_feature8	2939-2968	30	View Details
hCas9:T2A:Bsd	2969-7699	4731	View Details
misc_feature9	7700-7730	31	View Details
WPRE	7731-8328	598	View Details
misc_feature11	8329-8393	65	View Details
3' LTR-ΔU3	8394-8628	235	View Details
misc_feature10	8629-8700	72	View Details
SV40 early pA	8701-8835	135	View Details
misc_feature11	8836-9788	953	View Details
AmpiciIIin	9789-10649	861	View Details
misc_feature12	10650-10819	170	View Details
pUC ori	10820-11408	589	View Details
misc_feature13	11409-11874	466	View Details

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c0390d4ce4d87f445cbc910123b467fd

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