Vectors for Cas9(D10A)

Gene Information

  • Gene Name Cas9(D10A)
  • Type ORF
  • Subtype Cas protein
  • Description Human codon-optimized CRISPR associated protein 9 with D10A mutation, HA tag and nuclear localization signal
  • Application Notes Nickase; generates 5' overhang; can induce NHEJ with paired offset gRNAs; reduced off-target effect.
  • References Cell. 154: 1380 (2013)
  • View ORF sequence of this gene »

Popular Vector Designs for Cas9(D10A)(These vectors can be made very quickly)

Gene Expression Vectors
These designs are created with VectorBuilder’s standard backbones containing a ubiquitous promoter driving the ORF of your gene. A marker (e.g. EGFP) is placed in the vector where appropriate.
Vector Description Vector Type Marker Price
Cas9(D10A) expression regular plasmid vector Mammalian Gene Expression Vector   Guide  From $159
Cas9(D10A) expression lentivirus vector Mammalian Gene Expression Lentiviral Vector   Guide  From $159
Cas9(D10A) expression adenovirus vector Mammalian Gene Expression Adenoviral Vector   Guide  From $159
Cas9(D10A) expression AAV vector Mammalian Gene Expression AAV Vector   Guide  From $159
Cas9(D10A) expression PiggyBac vector Mammalian Gene Expression PiggyBac Vector   Guide  From $159

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