Lentivirus Packaging

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Lentivirus is one of the most commonly used vectors for efficient gene delivery into mammalian cells. VectorBuilder has developed a series of proprietary technologies and reagents that have greatly improved lentivirus packaging protocols in terms of titer, purity, viability and consistency, especially for the third-generation lentiviral vector systems used in our vector construction services. As a result, we have a growing base of highly satisfied customers who come back to us time after time for their lentiviral vector cloning and lentivirus packaging needs.
Click here to read detailed introduction of our lentiviral vector systems >>
Click here to view citations of our lentivirus >>
Click here to view user testimonials of our lentivirus >>
How do I obtain lentivirus packaging services?

Click here to view an example of a finished lentiviral vector design >>
Click here to view an example of a lentivirus service proposal >>
Click here to view instructions on designing a vector on VectorBuilder >>
Click here to view instructions on placing an order on VectorBuilder >>

Types of lentivirus offered

We offer packaging services for VSV-G pseudotyped second- and third-generation lentivirus. We can also package integrase-defective lentivirus (IDLV).

Price and turnaround

Due to our technology advantage, our lentivirus packaging services have the highest quality in the industry and are also faster and more affordable than those from other suppliers. Prices and turnaround times for different scales of lentivirus packaging are shown below:

Scale Application Titer & Volume* Price (USD) Turnaround**
Pilot Cell culture >108 TU/ml, 10x 25 ul, HBSS buffer $399 7-14 days
Medium Cell culture >108 TU/ml, 10x 100 ul, HBSS buffer $599 7-14 days
Large Cell culture >10TU/ml, 10x 100 ul, HBSS buffer $999 7-14 days
Ultra-purified medium Cell culture and in vivo >109 TU/ml, 10x 50 ul, HBSS buffer $1199 7-14 days
Ultra-purified large Cell culture and in vivo >109 TU/ml, 10x 100 ul, HBSS buffer $1499 7-14 days

* Our titer guarantee applies to vectors for which the region being packaged into virus (from Δ5’ LTR to ΔU3/3’ LTR) is below the lentivirus cargo limit (9.2 kb). For sizes above this, it may still be possible to package the vector into virus, but the titer may be reduced. Additionally, we are not able to guarantee titer for the following vectors:

  • vectors containing sequences that could adversely affect the packaging process such as toxic genes (e.g. proapoptotic genes), genes that compromise the integrity of packaging cells or virus (e.g. membrane proteins that cause cell aggregation), and sequences prone to rearrangements or secondary structures (e.g. repetitive or highly GC-rich sequences);
  • customer-supplied vectors as we have no control over their quality.

** Estimated turnaround is the time from production initiation to completion. It does not include waiting time for any customer-supplied materials (e.g. template DNA), QC of such materials, and transit time for shipping final deliverables to the customer.

Deliverables

If you choose pilot, medium or large scale lentivirus packaging, each order includes the following deliverables:

  • Aliquots of custom lentivirus at selected scale
  • Free: aliquots of control virus*
  • Free: Polybrene (5 mg/ml, 200 ul)

If you choose ultra-purified medium or ultra-purified large scale lentivirus packaging (in vivo grade), each order includes the following deliverables:

  • Aliquots of custom lentivirus at selected scale
  • "Add-on" purchase (optional): aliquots of ultra-purified control virus*
  • Free: Polybrene (5 mg/ml, 200 ul)
* The control virus is designed to match the biological application of the custom virus and to be used for testing transduction. For example, if the custom virus overexpresses a gene, then the control virus provided will overexpress EGFP, and if the custom virus expresses an shRNA against a gene, then the control virus provided will express a scramble shRNA. Detailed information for the various control viruses is shown below:
Vector System Control Vector Name Control Vector ID Custom Virus Packaging Scale Corresponding Control Virus Amount Price (USD)
Lentivirus gene expression system

pLV[Exp]-EGFP:T2A:
Puro-EF1A>mCherry

VB160109-10005 Pilot >108 TU/ml, 100 ul in HBSS Free
Medium >108 TU/ml, 2x 100 ul in HBSS Free
Large >108 TU/ml, 2x 100 ul in HBSS Free
Ultra-purified medium >109 TU/ml, 10x 50 ul in HBSS $400
Ultra-purified large >109 TU/ml, 10x 100 ul in HBSS $500
Lentivirus U6-based shRNA knockdown system

pLV[shRNA]-EGFP:T2A:
Puro-U6>Scramble_shRNA

VB151023-10034 Pilot >108 TU/ml, 100 ul in HBSS Free
Medium >108 TU/ml, 2x 100 ul in HBSS Free
Large >108 TU/ml, 2x 100 ul in HBSS Free
Ultra-purified medium >109 TU/ml, 10x 50 ul in HBSS $400
Ultra-purified large >109 TU/ml, 10x 100 ul in HBSS $500
Lentivirus miR30-based shRNA knockdown system

pLV[miR30]-EGFP/Puro-EF1A>mCherry:
Scramble_miR30-shRNA

VB180124-1174nde Pilot >108 TU/ml, 100 ul in HBSS Free
Medium >108 TU/ml, 2x 100 ul in HBSS Free
Large >108 TU/ml, 2x 100 ul in HBSS Free
Ultra-purified medium >109 TU/ml, 10x 50 ul in HBSS $400
Ultra-purified large >109 TU/ml, 10x 100 ul in HBSS $500
Lentivirus CRISPR system

pLV[Exp]-EGFP:
T2A:Puro-EF1A>mCherry

VB160109-10005 Pilot >108 TU/ml, 100 ul in HBSS Free
Medium >108 TU/ml, 2x 100 ul in HBSS Free
Large >108 TU/ml, 2x 100 ul in HBSS Free
Ultra-purified medium >109 TU/ml, 10x 50 ul in HBSS $400
Ultra-purified large >109 TU/ml, 10x 100 ul in HBSS $500
Production, purification and titration

For our third-generation lentivirus packaging, transfer plasmid carrying the gene of interest (GOI) is co-transfected with our proprietary envelope plasmid encoding VSV-G and packaging plasmids encoding Gag/Pol and Rev into packaging cells. After a short incubation period, the supernatant is collected and centrifuged to remove cell debris and then filtered. Viral particles are subsequently concentrated with PEG. For ultra-purification (in vivo grade), viral particles are further purified and concentrated by sucrose cushion centrifugation. We use the p24 ELISA method to measure lentivirus titer.

Read more about our titration method >>

Quality control

For each lentivirus produced by VectorBuilder, quality control includes titer measurement, sterility testing for bacteria and fungi, and mycoplasma detection. If the transfer vector encodes a fluorescent protein, we would transduce cells with virus and detect corresponding fluorescence. Additionally, for ultra-purified lentivirus, we routinely sample virus quality using the endotoxin assay.

Customer-supplied vectors

If customer-supplied lentiviral vectors are needed, please send us the vectors following the Materials Submission Guidelines. Please strictly follow our guidelines to set up shipment to avoid any delay or damage of materials. All customer-supplied materials undergo mandatory QC by VectorBuilder which may incur a $100 surcharge for each item. Please note that production may not be initiated until customer-supplied materials pass QC.

Questions

Please refer to our Vector FAQ for commonly asked technical questions. To get additional information about what we can offer, please contact VectorBuilder's service team following the contact information here.

Design My Vector 

Use this option to design your vector and
request cloning & downstream services.

Send Design Request 

User this option to ask our scientists to design your vector for you.