Plasmid DNA Preparation
High quality plasmid DNA is integral to many molecular biology techniques such as cloning, transfection/transformation, virus packaging, mutagenesis, protein production, Southern blot etc. VectorBuilder’s plasmid DNA preparation service allows you to obtain high-quality plasmid DNA on a desired scale in a rapid and cost-effective way. Our robust production and stringent quality control ensure that the plasmid DNA we provide is of high purity and can satisfy all kinds of downstream experiments.
How do I obtain plasmid DNA preparation service?
Click here to view instructions on placing an order on VectorBuilder
Price and turnaround time
|Miniprep||Molecular biology||DNA (>200 ng/ul, 50 ul, 1x TE buffer)||$39||3-5 days|
|Maxiprep||Molecular biology and cell culture||DNA (>1 ug/ul, 300 ul, 1x TE buffer, endotoxin-free, sterile)||$109||4-6 days|
|Megaprep||Molecular biology and cell culture||DNA (>1 ug/ul, 1 ml, 1x TE buffer, endotoxin-free, sterile)||$199**||4-6 days|
|Gigaprep||Molecular biology and cell culture||DNA (>1 ug/ul, 10 ml, 1x TE buffer, endotoxin-free, sterile)||$759**||6-8 days|
* Estimated turnaround is the time from production initiation to completion. It does not include waiting time for any customer-supplied materials (e.g. template DNA), QC of such materials, and transit time for shipping final deliverables to the customer.
** If you are interested in Megaprep and Gigaprep, please contact firstname.lastname@example.org for details. Discount may apply for bulk order.
Common QC we perform for plasmid DNA includes DNA quantification, A260/280 and A260/230 measurement, restriction enzyme digestion, Sanger sequencing, recovering bacteria from glycerol stocks, and re-transformation.
If customer-supplied vectors are needed, please send us the vectors following the Materials Submission Guidelines. Please strictly follow our guidelines to set up shipment to avoid any delay or damage of materials. All customer-supplied materials undergo mandatory QC by VectorBuilder which may incur a $100 surcharge for each item. Please note that production may not be initiated until customer-supplied materials pass QC.