Plasmid DNA Preparation

High quality plasmid DNA is integral to many molecular biology techniques such as cloning, transfection/transformation, virus packaging, mutagenesis, protein production, Southern blot etc. VectorBuilder’s plasmid DNA preparation service allows you to obtain high-quality plasmid DNA on a desired scale in a rapid and cost-effective way. Our robust production and stringent quality control ensure that the plasmid DNA we provide is of high purity and can satisfy all kinds of downstream experiments.
How do I obtain plasmid DNA preparation service?

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Click here to view instructions on placing an order on VectorBuilder >>
Price and turnaround time
Scale Application Deliverable Price (USD) Turnaround*
Miniprep Molecular biology DNA (>200 ng/ul, 50 ul, 1x TE buffer) $39 3-5 days
Maxiprep Molecular biology and cell culture DNA (>1 ug/ul, 300 ul, 1x TE buffer, endotoxin-free, sterile) $109 4-6 days

* Estimated turnaround is the time from production initiation to completion. It does not include waiting time for any customer-supplied materials (e.g. template DNA), QC of such materials, and transit time for shipping final deliverables to the customer.

Quality control

Common QC we perform for plasmid DNA includes DNA quantification, A260/280 and A260/230 measurement, restriction enzyme digestion, Sanger sequencing, recovering bacteria from glycerol stocks, and re-transformation.

Customer-supplied vectors

If customer-supplied vectors are needed, please send us the vectors following the Materials Submission Guidelines. Please strictly follow our guidelines to set up shipment to avoid any delay or damage of materials. All customer-supplied materials undergo mandatory QC by VectorBuilder which may incur a $100 surcharge for each item. Please note that production may not be initiated until customer-supplied materials pass QC.

Questions

Please refer to our Vector FAQ for commonly asked technical questions. To get additional information about what we can offer, please contact VectorBuilder's service team following the contact information here.