Plasmid DNA Preparation
High quality plasmid DNA is integral to many molecular biology techniques such as cloning, transfection/transformation, virus packaging, mutagenesis, protein production, Southern blot etc. VectorBuilder’s plasmid DNA preparation service allows you to obtain high-quality plasmid DNA on a desired scale in a rapid and cost-effective way. The scales of plasmid DNA we provide range from ug to g, satisfying a vast majority of needs from basic research scientists and industrial customers. In addition to research-grade plasmid, we also offer GMP-like and GMP-grade plasmid manufacturing for clinical applications.
Service Information
Price and turnaround Price Match
High Copy Plasmid
Medium/Low Copy Plasmid
| Scale | Application | Deliverable | Price (USD) | Turnaround* |
|---|---|---|---|---|
| Miniprep | Molecular biology | DNA (>200 ng/ul, 50 ul) | $29 | 1-2 days |
| Midiprep | Molecular biology and cell culture | DNA (>1 ug/ul, 100 ul, endotoxin-free, sterile) | $99 | 2-4 days
|
| Maxiprep | DNA (>1 ug/ul, 300 ul, endotoxin-free, sterile) | $149 | ||
| Megaprep** | DNA (>1 ug/ul, 1 ml, endotoxin-free, sterile) | $249 | ||
| Gigaprep** | DNA (>1 ug/ul, 10 ml, endotoxin-free, sterile) | $799 | 6-8 days | |
| Industrial grade*** | Various basic research and preclinical applications | DNA (10 mg to 1 g) | Please inquire | |
| Scale | Application | Deliverable | Price (USD) | Turnaround* |
|---|---|---|---|---|
| Miniprep | Molecular biology | DNA (>100 ng/ul, 50 ul) | $29 | 1-2 days |
| Midiprep | Molecular biology and cell culture | DNA (>500 ng/ul, 100 ul, endotoxin-free, sterile) | $99 | 2-4 days
|
| Maxiprep | DNA (>500 ng/ul, 300 ul, endotoxin-free, sterile) | $149 | ||
| Megaprep** | DNA (>500 ng/ul, 1 ml, endotoxin-free, sterile) | $249 | ||
| Gigaprep** | DNA (>500 ng/ul, 10 ml, endotoxin-free, sterile) | $799 | 6-8 days | |
| Industrial grade*** | Various basic research and preclinical applications | DNA (10 mg to 1 g) | Please inquire | |
* Estimated turnaround is the time from production initiation to completion. It does not include waiting time for any customer-supplied materials (e.g. template DNA), QC of such materials, and transit time for shipping final deliverables to the customer.
** If you are interested in Megaprep and Gigaprep services, please send a design request to us for details. Discount may apply for bulk order.
*** Industrial grade plasmid can achieve higher supercoiled content (90%±10%) and lower endotoxin level (<0.01 EU/ug). Additionally, animal-free plasmid prep can be provided upon request.
Shipping and storage
Our plasmid DNA is stored in 1x TE buffer. Plasmid DNA can be stored at -80°C for up to 12 months. Avoid freeze-thaw cycles.
Animal-free plasmid DNA preparation
In addition to the above-mentioned scales, we offer animal-products free plasmid DNA preparation services to provide you with plasmids that are free of transmissible spongiform encephalopathies (TSE) including bovine spongiform encephalopathy (BSE). Our production process has been optimized so no animal-derived components are used during manufacturing. The culture media we use consists of a plant-based tryptone substitute which replaces the traditional bovine-derived tryptone. Our DNA purification is conducted via non-enzymatic processes to circumvent the usage of RNase. The plasmid preparation is performed at separate lab stations by trained technicians to ensure the plasmids are free of contamination. TSE/BSE statement is available for supporting your regulatory submission. Please send a design request to us to get a detailed service proposal.
Quality control (QC)
Common QC we perform for plasmid DNA includes DNA quantification, A260/280 and A260/230 measurement, restriction enzyme digestion, Sanger sequencing, recovering bacteria from glycerol stocks, and re-transformation.
Customer-supplied materials
If customer-supplied vectors are needed, please send us the vectors following the Materials Submission Guidelines. Please strictly follow our guidelines to set up shipment to avoid any delay or damage of materials. All customer-supplied materials undergo mandatory QC by VectorBuilder which may incur a $50 surcharge for each item. Please note that production may not be initiated until customer-supplied materials pass QC.
How to Order
Customer-supplied vectors
If customer-supplied vectors are used in plasmid DNA preparation, please send us a design request and attach the sequence or map for the vectors. Click here to view instructions on placing an order on VectorBuider.