Vector Academy
Vector Academy is our knowledge base that serves as your resource for diving deeper into all things cloning, viruses, and gene delivery. We’re excited to share our expertise and what we’ve learned from decades of collective experience with you.
Optimizing CRISPR: Technology and Approaches for High Efficiency Gene Editing
Keywords: CRISPR-Cas9, genome editing, CRISPR vector design, gene knockout, gene regulation
An overview of CRISPR-Cas9 tools and mechanisms, with practical tips to optimize vector design for efficient genome editing.
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Design Pitfall: Using The Same Promoter To Drive Two Genes In Lentiviral Vector
Keywords: Lentivirus, Vector Design, Repetitive Sequences
Using two identical promoters in a lentiviral vector causes detrimental effects on proper expression of the two open reading frames.
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CRISPR This Way: Choosing Between Delivery Systems
Keywords: CRISPR delivery, plasmid, virus, mRNA, RNP
A comparison of delivery systems to help you choose the optimal method for your CRISPR experiments, from research applications to clinical use.
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Unleashing the Power of Oncolytic Viruses: Design Strategies for Maximum Efficacy
Keywords: Oncolytic virus, tumor targeting, immune stimulation
A deep dive into the attributes and design of recombinant viruses for use as potent oncolytic agents in the clinic and why HSV and VACV are ideal oncolytic virus candidates.
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Shine Bright, Little Cell: A Comprehensive Guide to Choosing the Right Reporter Gene
Keywords: Reporter genes, fluorescent reporters, luciferase assay
Discover primary reporter types and navigate the roadmap to find whether fluorescent reporters, luciferase, or beta-galactosidase is the best option to detect and measure your gene or element of interest.
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Me First! How Do I Optimize a Vector with Multiple Genes?
Keywords: Bicistronic vector, 2A peptide, linker
This guide provides key considerations and troubleshooting tips for constructing vectors with multiple genes, including which linker to use and the importance of optimizing order.
Read More ››Below you can view posts divided into introductory, experienced, and expert levels.
Lessons in Gene Delivery
An introduction to concepts and tools in gene delivery from mRNA and proteins to plasmids and viruses.
Gene Delivery in the Spotlight
From the news desk to VectorBuilder's labs, we highlight what is new and exciting in the field of gene delivery.
Troubleshooting Guides
A collection of tips and hypotheses based on the experience of the experts here at VectorBuilder.