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Vector Academy

Vector Academy is our knowledge base that serves as your resource for diving deeper into all things cloning, viruses, and gene delivery. We’re excited to share our expertise and what we’ve learned from decades of collective experience with you.
Latest Posts
Design Pitfall: Using The Same Promoter To Drive Two Genes In Lentiviral Vector

Keywords: Lentivirus, Vector Design, Repetitive Sequences

Using two identical promoters in a lentiviral vector causes detrimental effects on proper expression of the two open reading frames.

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CRISPR This Way: Choosing Between Delivery Systems

Keywords: CRISPR delivery, plasmid, virus, mRNA, RNP

A comparison of delivery systems to help you choose the optimal method for your CRISPR experiments, from research applications to clinical use.

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Unleashing the Power of Oncolytic Viruses: Design Strategies for Maximum Efficacy

Keywords: Oncolytic virus, tumor targeting, immune stimulation

A deep dive into the attributes and design of recombinant viruses for use as potent oncolytic agents in the clinic and why HSV and VACV are ideal oncolytic virus candidates.

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Shine Bright, Little Cell: A Comprehensive Guide to Choosing the Right Reporter Gene

Keywords: Reporter genes, fluorescent reporters, luciferase assay

Discover primary reporter types and navigate the roadmap to find whether fluorescent reporters, luciferase, or beta-galactosidase is the best option to detect and measure your gene or element of interest.

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Me First! How Do I Optimize a Vector with Multiple Genes?

Keywords: Bicistronic vector, 2A peptide, linker

This guide provides key considerations and troubleshooting tips for constructing vectors with multiple genes, including which linker to use and the importance of optimizing order.

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Driving CAR-T Therapy in the Clinic and with CRISPR

Keywords: CAR-T cell therapy, Kymriah CAR-T, immunotherpy

A deep dive into the biology behind CAR-T vectors, their clinical applications, and how CRISPR/Cas9 is being used to create the next generation of CAR therapeutics.

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Below you can view posts divided into introductory, experienced, and expert levels.
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