Tools Overview Sequence Design shRNA Target Design Primer Design Sequence Analysis Sequence Alignment Sequence Dot Plot GC Content Calculator DNA Secondary Structure DNA Reverse Complement Coding Sequence Analysis Codon Table Codon Optimization DNA Translation Bio Calculator DNA Mass - Moles Converter RNA Mass - Moles Converter Protein Mass - Moles Converter OD260 to Concentration Mass Calculator from Molarity Dilution Calculator OD600 Calculator Database ORF Search How to Use Vector Design Studio About VectorBuilder
Tools Sequence Design shRNA Target Design Design optimal shRNA sequences by knockdown score and position for your target sequence. Primer Design Design primer sequences optimized for melting temperature, GC content, and target specificity for your desired region. Sequence Analysis Sequence Alignment Pair-wise comparison of DNA, protein, or translation-based DNA sequences. Sequence Dot Plot Two-dimensional plot for identifying regions with similar features between two DNA or RNA sequences. GC Content Calculator Calculate GC content of entire sequence and specific regions. DNA Secondary Structure Predict secondary structures from base pair interactions. DNA Reverse Complement Retrieve reverse, complement, or reverse complement sequences. Coding Sequence Analysis Codon Table Obtain codon table with species-specific codon frequencies. Codon Optimization Optimize codons according to species, cleavage sites, and clonability. DNA Translation Retrieve amino acid sequence from DNA input. Bio Calculator DNA Mass - Moles Converter Calculate molecular weight and amount of your DNA (mass and moles). RNA Mass - Moles Converter Calculate molecular weight and amount of your RNA (mass and moles). Protein Mass - Moles Converter Calculate molecular weight and amount of your protein (mass and moles). OD260 to Concentration Input your DNA or RNA OD260 to convert to concentration. Mass Calculator from Molarity Calculate the mass needed of a compound to generate a solution at a desired molarity. Dilution Calculator Calculate the amount of stock solution needed to achieve a desired concentration and volume. OD600 Calculator Input your OD600 and convert to number of bacterial cells. Database ORF Search Find and retrieve ORFs for gene identification, annotation, and functional studies.
