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Tools

shRNA and CRISPR Design

shRNA Target Design
Design optimal shRNA sequences by knockdown score and position for your target sequence.

Sequence Analysis

Sequence Alignment
Pair-wise comparison of DNA, protein, or translation-based DNA sequences.
Sequence Dot Plot
Two-dimensional plot for identifying regions with similar features between two DNA or RNA sequences.
GC Content Calculator
Calculate GC content of entire sequence and specific regions.
DNA Secondary Structure
Predict secondary structures from base pair interactions.
DNA Reverse Complement
Retrieve reverse, complement, or reverse complement sequences.

Coding Sequence Analysis

Codon Table
Obtain codon table with species-specific codon frequencies.
Codon Optimization
Optimize codons according to species, cleavage sites, and clonability.
DNA Translation
Retrieve amino acid sequence from DNA input.
Design My Vector