How to Use Vector Design Studio_VectorBuilder

How to Use
Vector Design Studio

About VectorBuilder

About VectorBuilder

Tools

Sequence Design

shRNA Target Design
Design optimal shRNA sequences by knockdown score and position for your target sequence.
gRNA Target Design
Design gRNA sequences optimized for specificity.
Primer Design
Design primer sequences optimized for melting temperature, GC content, and target specificity for your desired region.

Sequence Analysis

Sequence Alignment
Pair-wise comparison of DNA, protein, or translation-based DNA sequences.
Sequence Dot Plot
Two-dimensional plot for identifying regions with similar features between two DNA or RNA sequences.
GC Content Calculator
Calculate GC content of entire sequence and specific regions.
DNA Secondary Structure
Predict secondary structures from base pair interactions.
DNA Reverse Complement
Retrieve reverse, complement, or reverse complement sequences.

Coding Sequence Analysis

Codon Table
Obtain codon table with species-specific codon frequencies.
Codon Optimization
Optimize codons according to species, cleavage sites, and clonability.
DNA Translation
Retrieve amino acid sequence from DNA input.

Bio Calculator

DNA Mass - Moles Converter
Calculate molecular weight and amount of your DNA (mass and moles).
RNA Mass - Moles Converter
Calculate molecular weight and amount of your RNA (mass and moles).
Protein Mass - Moles Converter
Calculate molecular weight and amount of your protein (mass and moles).
OD260 to Concentration
Input your DNA or RNA OD260 to convert to concentration.
Mass Calculator from Molarity
Calculate the mass needed of a compound to generate a solution at a desired molarity.
Dilution Calculator
Calculate the amount of stock solution needed to achieve a desired concentration and volume.
OD600 Calculator
Input your OD600 and convert to number of bacterial cells.

Database

ORF Search
Find and retrieve ORFs for gene identification, annotation, and functional studies.
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