Vectors for eNpHR3.0/EYFP

Gene Information

  • Gene Name eNpHR3.0/EYFP
  • Type ORF
  • Description Third generation of Halorhodopsin from Natronomonas pharaonis (NpHR) which is fused with EYFP. NpHR is a fast light-activated electrogenic Cl- pump. There is an nAChR-derived signal peptide tagged to the N-terminal of EYFP, and a Kir2.1-derived ER export signal peptide tagged to the C-terminal of EYFP to facilitate cell surface expression of NpHR
  • Application Notes Light activation of NpHR leads to cell hyperpolarization and inhibition of firing action potentials; increased peak photocurrent compared to original NpHR; no aggregation; low cellular toxicity; maximum excitation is 580 nm; maximum excitation and emission of EYFP are 513 nm and 527 nm, respectively.
  • References Front Behav Neurosci. 9: 286. (2015)
  • View ORF sequence of this gene »

Premade Vectors for eNpHR3.0/EYFP (These vectors are in stock and available immediately)

Gene Expression Vectors
These premade vectors are constructed with VectorBuilder’s standard backbones containing a ubiquitous promoter driving the ORF of your gene. A marker (e.g. EGFP) is placed in the vector where appropriate.
Vector Description Vector Type Marker Price
pAAV[Exp]-CMV>eNpHR3.0/EYFP:WPRE Mammalian Gene Expression AAV Vector - $159.00 View Vector 

Popular Vector Designs for eNpHR3.0/EYFP(These vectors can be made very quickly)

Gene Expression Vectors
These designs are created with VectorBuilder’s standard backbones containing a ubiquitous promoter driving the ORF of your gene. A marker (e.g. EGFP) is placed in the vector where appropriate.
Vector Description Vector Type Marker Price
eNpHR3.0/EYFP expression regular plasmid vector Mammalian Gene Expression Vector   Guide  From $159
eNpHR3.0/EYFP expression lentivirus vector Mammalian Gene Expression Lentiviral Vector   Guide  From $159
eNpHR3.0/EYFP expression adenovirus vector Mammalian Gene Expression Adenoviral Vector   Guide  From $159
eNpHR3.0/EYFP expression AAV vector Mammalian Gene Expression AAV Vector   Guide  From $159
eNpHR3.0/EYFP expression PiggyBac vector Mammalian Gene Expression PiggyBac Vector   Guide  From $159

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