Vectors for hCas9

Gene Information

  • Gene Name hCas9
  • Type ORF
  • Subtype Cas protein
  • Description Human codon-optimized CRISPR associated protein 9 from Streptococcus pyogenes (with 3xFLAG tag and nuclear localization signal), also known as SpCas9
  • Application Notes Generates double-strand DNA breaks.
  • References Science. 339:819 (2013)
  • View ORF sequence of this gene »

Premade Vectors for hCas9 (These vectors are in stock and available immediately)

Gene Expression Vectors
These premade vectors are constructed with VectorBuilder’s standard backbones containing a ubiquitous promoter driving the ORF of your gene. A marker (e.g. EGFP) is placed in the vector where appropriate.
Vector Description Vector Type Marker Price
pLV[Exp]-mCherry-CBh>hCas9 Mammalian Gene Expression Lentiviral Vector mCherry $159.00 View Vector 
pLV[Exp]-mCherry/Neo-CBh>hCas9 Mammalian Gene Expression Lentiviral Vector mCherry/Neo $159.00 View Vector 
pLV[Exp]-CBh>hCas9 Mammalian Gene Expression Lentiviral Vector - $159.00 View Vector 
pPB[Exp]-mCherry/Hygro-CBh>hCas9 Mammalian Gene Expression PiggyBac Vector mCherry/Hygro $159.00 View Vector 
pLV[Exp]-EGFP:T2A:Puro-EFS>hCas9 Mammalian Gene Expression Lentiviral Vector EGFP:T2A:Puro $159.00 View Vector 
pPB[Exp]-EGFP/Puro-CAG>hCas9 Mammalian Gene Expression PiggyBac Vector EGFP/Puro $159.00 View Vector 
pAV[Exp]-CBh>hCas9 Mammalian Gene Expression Adenoviral Vector - $159.00 View Vector 
pLV[Exp]-Hygro-CMV>hCas9 Mammalian Gene Expression Lentiviral Vector Hygro $159.00 View Vector 
pLV[Exp]-mCherry:T2A:Puro-CBh>hCas9 Mammalian Gene Expression Lentiviral Vector mCherry:T2A:Puro $159.00 View Vector 
pRP[Exp]-CBh>hCas9 Mammalian Gene Expression Vector - $159.00 View Vector 

Popular Vector Designs for hCas9(These vectors can be made very quickly)

Gene Expression Vectors
These designs are created with VectorBuilder’s standard backbones containing a ubiquitous promoter driving the ORF of your gene. A marker (e.g. EGFP) is placed in the vector where appropriate.
Vector Description Vector Type Marker Price
hCas9 expression regular plasmid vector Mammalian Gene Expression Vector   Guide  From $159
hCas9 expression lentivirus vector Mammalian Gene Expression Lentiviral Vector   Guide  From $159
hCas9 expression adenovirus vector Mammalian Gene Expression Adenoviral Vector   Guide  From $159
hCas9 expression AAV vector Mammalian Gene Expression AAV Vector   Guide  From $159
hCas9 expression PiggyBac vector Mammalian Gene Expression PiggyBac Vector   Guide  From $159

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  • Virus packaging
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