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Vectors for mt-mKeima

Gene Information

  • Gene Name mt-mKeima
  • Type CDS
  • Subtype pH-Sensitive Fluorescent Reporters
  • Description Monomeric dual-excitation Keima protein derived from stony coral Montipora by numerous cycles of semi-random mutagenesis; fused with a tandem repeat of mitochondria-targeting sequences from COX VIII
  • Application Notes Localizes to the mitochondrial matrix for the detection of mitophagy using reporter color change. The excitation wavelength of the mKeima fluorescent protein is determined by the pH of the environment, with an excitation wavelength centered at 440 nm at neutral pH and an excitation of 586 nm at an acidic pH. The emission wavelength of Keima is identical at either pH and peaks maximally at 620 nm.
  • References Chem Biol.18:1042 (2011); Nat Protoc.12:1576 (2017)
  • View ORF sequence of this gene Â»

Premade Vectors for mt-mKeima (These vectors are in stock and available immediately)

Gene Expression Vectors
These premade vectors are constructed with VectorBuilder’s standard backbones containing a ubiquitous promoter driving the ORF of your gene. A marker (e.g. EGFP) is placed in the vector where appropriate.
Vector Description Vector Type Marker Price
pLV[Exp]-EFS>mt-mKeima Mammalian Gene Expression Lentiviral Vector - $189.00 View Vector 
pLV[Exp]-Neo-EF1A>mt-mKeima Mammalian Gene Expression Lentiviral Vector Neo $189.00 View Vector 
pRP[Exp]-Neo-CAG>mt-mKeima Mammalian Gene Expression Vector Neo $189.00 View Vector 
pAAV[Exp]-CAG>mt-mKeima:WPRE Mammalian Gene Expression AAV Vector - $189.00 View Vector 
pPB[Exp]-Puro-EF1A>mt-mKeima Mammalian Gene Expression PiggyBac Vector Puro $189.00 View Vector 
pLV[Exp]-Puro-MSCV>mt-mKeima Mammalian Gene Expression Lentiviral Vector Puro $189.00 View Vector 
pRP[Exp]-Puro-CAG>mt-mKeima Mammalian Gene Expression Vector Puro $189.00 View Vector 
pLV[Exp]-Bsd-CAG>mt-mKeima Mammalian Gene Expression Lentiviral Vector Bsd   View Vector 
pRP[Exp]-CMV>mt-mKeima Mammalian Gene Expression Vector - $189.00 View Vector 
pRP[Exp]-Puro-EF1A>mt-mKeima Mammalian Gene Expression Vector Puro $189.00 View Vector 
pAV[Exp]-CMV>mt-mKeima Mammalian Gene Expression Adenoviral Vector - $189.00 View Vector 
pPB[Exp]-Bsd-EF1A>mt-mKeima Mammalian Gene Expression PiggyBac Vector Bsd $189.00 View Vector 

Popular Vector Designs for mt-mKeima(These vectors can be made very quickly)

Gene Expression Vectors
These designs are created with VectorBuilder’s standard backbones containing a ubiquitous promoter driving the ORF of your gene. A marker (e.g. EGFP) is placed in the vector where appropriate.
Vector Description Vector Type Marker Price
mt-mKeima expression regular plasmid vector Mammalian Gene Expression Vector   Guide 
From $189
mt-mKeima expression lentivirus vector Mammalian Gene Expression Lentiviral Vector   Guide 
From $189
mt-mKeima expression adenovirus vector Mammalian Gene Expression Adenoviral Vector   Guide 
From $189
mt-mKeima expression AAV vector Mammalian Gene Expression AAV Vector   Guide 
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From $189
mt-mKeima expression PiggyBac vector Mammalian Gene Expression PiggyBac Vector   Guide 
From $189

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