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Vectors for tTS/rtTA

Gene Information

  • Gene Name tTS/rtTA
  • Type ORF
  • Subtype Tet regulatory protein
  • Description tTS and rtTA_M2 linked by T2A
  • Application Notes In the absence of tetracycline or its analogs (e.g. doxycycline), tTS binds to TRE promoter to actively suppress gene transcription whereas rtTA_M2 is unable to bind to TRE promoter. In the presence of the inducing drug, tTS dissociates from TRE promoter whereas rtTA_M2 binds to TRE promoter to activate gene transcription. Compared to using rtTA_M2 alone, which does not provide active repression, this system has lower leaky expression in the absence of the inducing drug.
  • References Engineered by VectorBuilder based on Semin Cell Dev Biol. 13:121 (2002)
  • View ORF sequence of this gene Â»

Premade Vectors for tTS/rtTA (These vectors are in stock and available immediately)

Gene Expression Vectors
These premade vectors are constructed with VectorBuilder’s standard backbones containing a ubiquitous promoter driving the ORF of your gene. A marker (e.g. EGFP) is placed in the vector where appropriate.
Vector Description Vector Type Marker Price
pRP[Exp]-EGFP/Neo-CMV>tTS/rtTA:IRES:ORF_Stuffer Mammalian Gene Expression Vector EGFP/Neo $189.00 View Vector 
pLV[Exp]-mCherry/Neo-EF1A>tTS/rtTA Mammalian Gene Expression Lentiviral Vector mCherry/Neo $189.00 View Vector 
pLV[Exp]-EF1A>tTS/rtTA:IRES:mCherry Mammalian Gene Expression Lentiviral Vector - $189.00 View Vector 
pAAV[Exp]-CMV>tTS/rtTA:WPRE Mammalian Gene Expression AAV Vector - $189.00 View Vector 
pPB[Exp]-CAG>tTS/rtTA:IRES:Hygro Mammalian Gene Expression PiggyBac Vector - $189.00 View Vector 
pPB[Exp]-UBC>tTS/rtTA:IRES:Hygro Mammalian Gene Expression PiggyBac Vector - $189.00 View Vector 
pAAV[Exp]-EF1A>tTS/rtTA:WPRE Mammalian Gene Expression AAV Vector - $189.00 View Vector 
pAAV[Exp]-CMV>tTS/rtTA:IRES:Puro:WPRE Mammalian Gene Expression AAV Vector - $189.00 View Vector 
pPB[Exp]-EF1A>tTS/rtTA:IRES:Neo Mammalian Gene Expression PiggyBac Vector - $189.00 View Vector 
pAAV[Exp]-CBh>tTS/rtTA:WPRE Mammalian Gene Expression AAV Vector - $189.00 View Vector 
pRP[Exp]-Neo-CMV>tTS/rtTA Mammalian Gene Expression Vector Neo $189.00 View Vector 

Popular Vector Designs for tTS/rtTA(These vectors can be made very quickly)

Gene Expression Vectors
These designs are created with VectorBuilder’s standard backbones containing a ubiquitous promoter driving the ORF of your gene. A marker (e.g. EGFP) is placed in the vector where appropriate.
Vector Description Vector Type Marker Price
tTS/rtTA expression regular plasmid vector Mammalian Gene Expression Vector   Guide 
From $189
tTS/rtTA expression lentivirus vector Mammalian Gene Expression Lentiviral Vector   Guide 
From $189
tTS/rtTA expression adenovirus vector Mammalian Gene Expression Adenoviral Vector   Guide 
From $189
tTS/rtTA expression AAV vector Mammalian Gene Expression AAV Vector   Guide 
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From $189
tTS/rtTA expression PiggyBac vector Mammalian Gene Expression PiggyBac Vector   Guide 
From $189

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