Vectors for tTS/rtTA

Gene Information

  • Gene Name tTS/rtTA
  • Type ORF
  • Subtype Regulatory protein
  • Description tTS and rtTA_M2 linked by T2A
  • Application Notes In the absence of tetracycline or its analogs (e.g. doxycycline), tTS binds to TRE promoter to actively suppress gene transcritpion whereas rtTA_M2 is unable to bind to TRE promoter. In the presence of the inducing drug, tTS dissociates from TRE promoter whereas rtTA_M2 binds to TRE promoter to activate gene transcription. Compared to using rtTA_M2 alone, which does not provide active repression, this system has lower leaky expression in the absence of the inducing drug.
  • References Engineered by VectorBuilder based on Semin Cell Dev Biol. 13:121 (2002)
  • View ORF sequence of this gene »

Premade Vectors for tTS/rtTA (These vectors are in stock and available immediately)

Gene Expression Vectors
These premade vectors are constructed with VectorBuilder’s standard backbones containing a ubiquitous promoter driving the ORF of your gene. A marker (e.g. EGFP) is placed in the vector where appropriate.
Vector Description Vector Type Marker Price
pRP[Exp]-Neo-CMV>tTS/rtTA Mammalian Gene Expression Vector Neo $159.00 View Vector 
pLV[Exp]-Bsd-UBC>tTS/rtTA Mammalian Gene Expression Lentiviral Vector Bsd $159.00 View Vector 
pLV[Exp]-EF1A>tTS/rtTA:IRES:EGFP Mammalian Gene Expression Lentiviral Vector - $159.00 View Vector 
pLV[Exp]-Bsd-EF1A>tTS/rtTA Mammalian Gene Expression Lentiviral Vector Bsd $159.00 View Vector 
pLV[Exp]-Neo-CMV>tTS/rtTA Mammalian Gene Expression Lentiviral Vector Neo $159.00 View Vector 
pLV[Exp]-Puro-CBh>tTS/rtTA Mammalian Gene Expression Lentiviral Vector Puro $159.00 View Vector 
pLV[Exp]-Puro-EF1A>tTS/rtTA Mammalian Gene Expression Lentiviral Vector Puro $159.00 View Vector 
pLV[Exp]-Hygro-UBC>tTS/rtTA Mammalian Gene Expression Lentiviral Vector Hygro $159.00 View Vector 
pLV[Exp]-Neo-CBh>tTS/rtTA Mammalian Gene Expression Lentiviral Vector Neo $159.00 View Vector 
pLV[Exp]-EGFP/Neo-EFS>tTS/rtTA Mammalian Gene Expression Lentiviral Vector EGFP/Neo $159.00 View Vector 

Popular Vector Designs for tTS/rtTA(These vectors can be made very quickly)

Gene Expression Vectors
These designs are created with VectorBuilder’s standard backbones containing a ubiquitous promoter driving the ORF of your gene. A marker (e.g. EGFP) is placed in the vector where appropriate.
Vector Description Vector Type Marker Price
tTS/rtTA expression regular plasmid vector Mammalian Gene Expression Vector   Guide  From $159
tTS/rtTA expression lentivirus vector Mammalian Gene Expression Lentiviral Vector   Guide  From $159
tTS/rtTA expression adenovirus vector Mammalian Gene Expression Adenoviral Vector   Guide  From $159
tTS/rtTA expression AAV vector Mammalian Gene Expression AAV Vector   Guide  From $159
tTS/rtTA expression PiggyBac vector Mammalian Gene Expression PiggyBac Vector   Guide  From $159

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