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  3. Vectors for eNpHR2.0/EYFP

Vectors for eNpHR2.0/EYFP

Gene Information

  • Gene Name eNpHR2.0/EYFP
  • Type ORF
  • Subtype Optogenetic protein
  • Description Enhanced Halorhodopsin from Natronomonas pharaonis (NpHR) which is fused with EYFP. NpHR is a fast light-activated electrogenic Cl- pump. There is an nAChR-derived signal peptide tagged to the N-terminal of NpHR, and a Kir2.1-derived ER export signal peptide tagged to the C-terminal of EYFP to facilitate cell surface expression of NpHR
  • Application Notes Light activation of NpHR leads to cell hyperpolarization and inhibition of firing action potentials; increased peak photocurrent compared to original NpHR; no aggregation; low cellular toxicity; maximum excitation is 580 nm; maximum excitation and emission of EYFP are 513 nm and 527 nm, respectively.
  • References Nature. 446:633 (2007); Brain Cell Biol. 36:129 (2008)
  • View ORF sequence of this gene Â»

Premade Vectors for eNpHR2.0/EYFP (These vectors are in stock and available immediately)

Gene Expression Vectors
These premade vectors are constructed with VectorBuilder’s standard backbones containing a ubiquitous promoter driving the ORF of your gene. A marker (e.g. EGFP) is placed in the vector where appropriate.
Vector Description Vector Type Marker Price
pRP[Exp]-mCherry-CAG>eNpHR2.0/EYFP Mammalian Gene Expression Vector mCherry $189.00 View Vector 

Popular Vector Designs for eNpHR2.0/EYFP(These vectors can be made very quickly)

Gene Expression Vectors
These designs are created with VectorBuilder’s standard backbones containing a ubiquitous promoter driving the ORF of your gene. A marker (e.g. EGFP) is placed in the vector where appropriate.
Vector Description Vector Type Marker Price
eNpHR2.0/EYFP expression regular plasmid vector Mammalian Gene Expression Vector   Guide 
From $189
eNpHR2.0/EYFP expression lentivirus vector Mammalian Gene Expression Lentiviral Vector   Guide 
From $189
eNpHR2.0/EYFP expression adenovirus vector Mammalian Gene Expression Adenoviral Vector   Guide 
From $189
eNpHR2.0/EYFP expression AAV vector Mammalian Gene Expression AAV Vector   Guide 
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From $189
eNpHR2.0/EYFP expression PiggyBac vector Mammalian Gene Expression PiggyBac Vector   Guide 
From $189

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