Baculovirus is widely used for the expression of recombinant proteins in cultured insect cells and mammalian cells. It is one of the most versatile and powerful systems for eukaryotic expression of recombinant proteins, especially for those where maintenance of eukaryotic-specific post-translational modifications is critical. VectorBuilder has developed a series of proprietary technologies and reagents that have greatly improved baculovirus packaging protocols in terms of titer, purity, viability and consistency. As a result, we have a growing base of highly satisfied customers who come back to us time after time for their baculovirus vector cloning and packaging needs.Click here to read detailed introduction of our baculovirus vector systems >>
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How do I obtain baculovirus packaging services?
Click here to view an example of an baculovirus service proposal >>
Click here to view instructions on designing a vector on VectorBuilder >>
Click here to view instructions on placing an order on VectorBuilder >>
Types of baculovirus offered
We offer baculovirus packaging of baculovirus strain AcMNPV (Autographa californica multicapsid nucleopolyhedrovirus).
Price and turnaround
Due to our technological advantage, our baculovirus packaging services have the highest quality in the industry and are also faster and more affordable than those from other suppliers. Prices and turnaround times for different scales of baculovirus packaging are shown below:
|Scale||Application||Titer & Volume*||Price (USD)||Turnaround*|
|Medium||Cell culture||>106 PFU/ml, 10x 100 ul, DPBS buffer||$599||7-14 days|
|Large||Cell culture||>107 PFU/ml, 10x 100 ul, DPBS buffer||$999||14-21 days|
* Estimated turnaround is the time from production initiation to completion. It does not include waiting time for any customer-supplied materials (e.g. template DNA), QC of such materials, and transit time for shipping final deliverables to the customer.
Each order includes the following deliverables:
- Aliquots of custom baculovirus at selected scale
- Free: aliquots of control virus*
* The control virus is designed to match the biological application of the custom virus and to be used for testing transduction. For example, if the custom virus overexpresses a gene, then the control virus provided will overexpress EGFP. Detailed information for the control virus is shown below:
|Control Vector Name||Control Vector ID||Custom Virus Packaging Scale||Control Virus Amount|
|pBV-EGFP||VB170317-1029uux||Medium||>106 PFU/ml, 2x 100 ul in DPBS|
|Large||>106 PFU/ml, 2x 100 ul in DPBS|
Production and titration
For our baculovirus packaging, the transfer plasmid carrying the gene of interest (GOI) is co-transformed with a helper plasmid expressing the Tn7 transposase into the bacterial host harboring the empty baculovirus shuttle vector (a.k.a. bacmid) to generate recombinant bacmid. The recombinant bacmid is then transfected into insect Sf9 cells. After a short incubation period, viral particles are harvested from medium and further concentrated by sucrose cushion centrifugation. We use a qPCR-based approach to measure baculorivus titer.
For each baculovirus produced by VectorBuilder, quality control includes titer measurement, sterility testing for bacteria and fungi, and mycoplasma detection. If the transfer vector encodes a fluorescent protein, we would transduce cells with virus and detect corresponding fluorescence.
If customer-supplied baculovirus transfer vectors or recombinant bacmids are needed, please send us the vector DNA following the Materials Submission Guidelines. Please strictly follow our guidelines to set up shipment to avoid any delay or damage of materials. All customer-supplied materials undergo mandatory QC by VectorBuilder which may incur a $100 surcharge for each item. Please note that production may not be initiated until customer-supplied materials pass QC.