AAV Serotype Testing Panel
Adeno-associated viruses (AAVs) have emerged as the most effective viral vectors for gene therapy due to their ability to transduce a wide variety of mammalian cell types and their low immunogenicity in host organisms. VectorBuilder offers the AAV serotype testing panel to enable users to select the optimal AAV serotype for specific applications by systematic comparison of a variety of serotypes in cells or in animals.
Highlights
- Our AAV serotype testing panel is the only commercially available AAV serotype testing kit that offers you the flexibility to customize your AAV panel with 3 or more AAV units from our wide collection of 18 serotypes, based on your project needs.
- Furthermore, when ordering your AAV panel you have the option to select between either CAG or CMV promoter for driving AAV-mediated EGFP reporter expression, which allows you to monitor and compare the performance of different serotypes on the panel
Ordering Information
Technical Information
- AAV vector
- Tissue tropism of AAV serotypes
- Experimental validation
- Documents
Ordering InformationPrice Match
| Product Name | Catalog No. | Titer & Volume | Unit Price (USD) | Availability |
|---|---|---|---|---|
| In vitro grade AAV serotype testing panel (CMV-EGFP) | PANEL-AAVS01 | ~1012 GC/ml, 25 ul | $79 per aliquot | In stock |
| In vitro grade AAV serotype testing panel (CAG-EGFP) | PANEL-AAVS02 | |||
| In vivo grade AAV serotype testing panel (CMV-EGFP) | PANEL-AAVSP01 | ~1013 GC/ml, 25 ul | $139 per aliquot | |
| In vivo grade AAV serotype testing panel (CAG-EGFP) | PANEL-AAVSP02 |
Notes:
a. Our standard AAV serotype collection includes AAVs 1, 2, 3, 4, 5, 6, 6.2, 7, 8, 9, rh10, DJ, DJ/8, PHP.eB, PHP.S, AAV2-retro, AAV2-QuadYF and AAV2.7m8.
b. The AAVs packaged in this offering are ssAAVs. If you need scAAVs (self-complementary AAVs), please send a design request to us.
c. A minimum of three units with the same or different serotypes is required for each panel. To customize and order your panel, please send a design request to us.
Shipping and storage
Our non-ultra-purified AAV is stored in a Tris-based buffer and our ultra-purified AAV is stored in a PBS-based buffer. Upon receiving, they should be stored at -80°C for long term (stable for at least 1 year), or -20°C for short term, e.g. 2-3 weeks. Thawed AAV can be stored at 4 °C for 1-2 weeks.
Technical Information
AAV vector
The AAV serotype testing panel contains premade AAVs expressing EGFP using our highly optimized AAV vector system. Users have the option to select between either CAG or CMV promoter for driving EGFP expression. Our AAV vector system is optimized for high copy number replication in E. coli, high-titer packaging of live virus, efficient transduction of host cells, and high-level transgene expression. Our AAV vectors can be packaged into virus using all known capsid serotypes, is capable of very high transduction efficiency, and presents a low safety risk.
Figure 1. Maps of EGFP-expressing AAV vectors with (A) CMV promoter or (B) CAG promoter.
Tissue tropism of AAV serotypes
Many strains of AAV have been identified in nature. They are divided into different serotypes based on different antigenicity of the capsid protein on the viral surface. Different serotypes can render the virus with different tissue tropism (i.e. tissue specificity of infection). When our AAV vectors are packaged into the virus, different serotypes can be conferred to the virus by using different capsid proteins for the packaging. The AAV serotype testing panel is designed for a systematic comparison of the tissue specificity of multiple AAV serotypes to help you select the optimal serotype for your experiment. The tables below list the tissue specificity conferred by different AAV serotypes.
List by Serotype
List by Tissue Type
| Serotype | Tissue tropism |
|---|---|
| AAV1 | Smooth muscle, skeletal muscle, CNS, brain, lung, retina, inner ear, pancreas, heart, liver |
| AAV2 | Smooth muscle, CNS, brain, liver, pancreas, kidney, retina, inner ear, testes |
| AAV3 | Smooth muscle, liver, lung |
| AAV4 | CNS, retina, lung, kidney, heart |
| AAV5 | Smooth muscle, CNS, brain, lung, retina, heart |
| AAV6 | Smooth muscle, heart, lung, pancreas, adipose, liver |
| AAV6.2 | Lung, liver, inner ear |
| AAV7 | Smooth muscle, retina, CNS, brain, liver |
| AAV8 | Smooth muscle, CNS, brain, retina, inner ear, liver, pancreas, heart, kidney, adipose |
| AAV9 | Smooth muscle, skeletal muscle, lung, liver, heart, pancreas, CNS, retina, inner ear, testes, kidney, adipose |
| AAVrh10 | Smooth muscle, lung, liver, heart, pancreas, CNS, retina, kidney |
| AAV-DJ | Liver, heart, kidney, spleen |
| AAV-DJ/8 | Liver, brain, spleen, kidney |
| AAV-PHP.eB | CNS |
| AAV-PHP.S | PNS |
| AAV2-retro | Spinal nerves |
| AAV2-QuadYF | Endothelial cell, retina |
| AAV2.7m8 | Retina, inner ear |
| Tissue type | Recommended AAV serotypes |
|---|---|
| Smooth muscle | AAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10 |
| Skeletal muscle | AAV1, AAV9 |
| CNS | AAV1, AAV2, AAV4, AAV5, AAV7, AAV8, AAV9, AAVrh10, AAV-PHP.eB |
| PNS | AAV-PHP.S |
| Brain | AAV1, AAV2, AAV5, AAV7, AAV8, AAV-DJ/8 |
| Retina | AAV1, AAV2, AAV4, AAV5, AAV7, AAV8, AAV9, AAVrh10, AAV2-QuadYF, AAV2.7m8 |
| Inner ear | AAV1, AAV2, AAV6.2, AAV8, AAV9, AAV2.7m8 |
| Lung | AAV1, AAV3, AAV4, AAV5, AAV6, AAV6.2, AAV9, AAVrh10 |
| Liver | AAV1, AAV2, AAV3, AAV6, AAV6.2, AAV7, AAV8, AAV9, AAVrh10, AAV-DJ, AAV-DJ/8 |
| Pancreas | AAV1, AAV2, AAV6, AAV8, AAV9, AAVrh10 |
| Heart | AAV1,AAV4, AAV5, AAV6, AAV8, AAV9, AAVrh10, AAV-DJ |
| Kidney | AAV2, AAV4, AAV8, AAV9, AAVrh10, AAV-DJ, AAV-DJ/8 |
| Adipose | AAV6, AAV8, AAV9 |
| Testes | AAV2, AAV9 |
| Spleen | AAV-DJ, AAV-DJ/8 |
| Spinal nerves | AAV2-retro |
| Endothelial cells | AAV2-QuadYF |
Experimental validation
We have developed a number of proprietary techniques to optimize our triple transfection-based AAV packaging protocol and our virus has been validated to exhibit high transduction efficiency in mammalian cells.
Figure 2. HEK293T cells were transduced with 18 serotypes of recombinant AAV packaged from the same CMV>EGFP vector (VB010000-9394npt). Representative EGFP expression at 48 h post transduction in different serotypes is shown as indicated. Scale bars: 100 μm.
Figure 3. HeLa cells were transduced with 18 serotypes of recombinant AAV packaged from the same CMV>EGFP vector (VB010000-9394npt). EGFP (A) Mean Fluorescence Intensity (MFI) and (B) positivity were quantified using flow cytometry 48 h post transduction. (C) Representative EGFP expression in different serotypes is shown as indicated. Scale bars: 100 μm.
Figure 4. Huh-7 cells were transduced with 18 serotypes of recombinant AAV packaged from the same CMV>EGFP vector (VB010000-9394npt). EGFP (A) Mean Fluorescence Intensity (MFI) and (B) positivity were quantified using flow cytometry 48 h post transduction. (C) Representative EGFP expression in different serotypes is shown as indicated. Scale bars: 100 μm.