AAV Serotype Testing Panel

Adeno-associated viruses (AAVs) have emerged as the most effective viral vectors for gene therapy due to their ability to transduce a wide variety of mammalian cell types and their low immunogenicity in host organisms. VectorBuilder offers the AAV serotype testing panel to enable users to select the optimal AAV serotype for specific applications by systematic comparison of a variety of serotypes in cells or in animals. 

Highlights
  • Our AAV serotype testing panel is the only commercially available AAV serotype testing kit that offers you the flexibility to customize your AAV panel with 3 or more AAV units from our wide collection of 18 serotypes, based on your project needs.
  • Furthermore, when ordering your AAV panel you have the option to select between either CAG or CMV promoter for driving AAV-mediated EGFP reporter expression, which allows you to monitor and compare the performance of different serotypes on the panel
Ordering Information
Technical Information

Ordering InformationPrice Match

Product NameCatalog No.Titer & VolumeUnit Price (USD)Availability
In vitro grade AAV serotype testing panel (CMV-EGFP)PANEL-AAVS01~1012 GC/ml, 25 ul$79 per aliquotIn stock
In vitro grade AAV serotype testing panel (CAG-EGFP)PANEL-AAVS02
In vivo grade AAV serotype testing panel (CMV-EGFP)PANEL-AAVSP01~1013 GC/ml, 25 ul$139 per aliquot
In vivo grade AAV serotype testing panel (CAG-EGFP)PANEL-AAVSP02

Notes:

a. Our standard AAV serotype collection includes AAVs 1, 2, 3, 4, 5, 6, 6.2, 7, 8, 9, rh10, DJ, DJ/8, PHP.eB, PHP.S, AAV2-retro, AAV2-QuadYF and AAV2.7m8.

b. The AAVs packaged in this offering are ssAAVs. If you need scAAVs (self-complementary AAVs), please send a design request to us.

c. A minimum of three units with the same or different serotypes is required for each panel. To customize and order your panel, please send a design request to us.

Shipping and storage

Our non-ultra-purified AAV is stored in a Tris-based buffer and our ultra-purified AAV is stored in a PBS-based buffer. Upon receiving, they should be stored at -80°C for long term (stable for at least 1 year), or -20°C for short term, e.g. 2-3 weeks. Thawed AAV can be stored at 4 °C for 1-2 weeks.

Technical Information

AAV vector

The AAV serotype testing panel contains premade AAVs expressing EGFP using our highly optimized AAV vector system. Users have the option to select between either CAG or CMV promoter for driving EGFP expression. Our AAV vector system is optimized for high copy number replication in E. coli, high-titer packaging of live virus, efficient transduction of host cells, and high-level transgene expression. Our AAV vectors can be packaged into virus using all known capsid serotypes, is capable of very high transduction efficiency, and presents a low safety risk.

Figure 1. Maps of EGFP-expressing AAV vectors with (A) CMV promoter or (B) CAG promoter.
Tissue tropism of AAV serotypes

Many strains of AAV have been identified in nature. They are divided into different serotypes based on different antigenicity of the capsid protein on the viral surface. Different serotypes can render the virus with different tissue tropism (i.e. tissue specificity of infection). When our AAV vectors are packaged into the virus, different serotypes can be conferred to the virus by using different capsid proteins for the packaging. The AAV serotype testing panel is designed for a systematic comparison of the tissue specificity of multiple AAV serotypes to help you select the optimal serotype for your experiment. The tables below list the tissue specificity conferred by different AAV serotypes.

List by Serotype
List by Tissue Type
SerotypeTissue tropism
AAV1Smooth muscle, skeletal muscle, CNS, brain, lung, retina, inner earpancreas, heart, liver
AAV2Smooth muscle, CNS, brain, liver, pancreas, kidney, retina, inner ear, testes
AAV3Smooth muscle, liver, lung
AAV4CNS, retina, lung, kidney, heart
AAV5Smooth muscle, CNS, brain, lung, retina, heart
AAV6Smooth muscle, heart, lung, pancreas, adipose, liver
AAV6.2Lung, liver, inner ear
AAV7Smooth muscle, retina, CNS, brain, liver
AAV8Smooth muscle, CNS, brain, retina, inner earliver, pancreas, heart, kidney, adipose
AAV9Smooth muscle, skeletal muscle, lung, liver, heart, pancreas, CNS, retina, inner ear, testes, kidney, adipose
AAVrh10Smooth muscle, lung, liver, heart, pancreas, CNS, retina, kidney
AAV-DJLiver, heart, kidney, spleen
AAV-DJ/8Liver, brain, spleen, kidney
AAV-PHP.eBCNS
AAV-PHP.SPNS
AAV2-retroSpinal nerves 
AAV2-QuadYFEndothelial cell, retina
AAV2.7m8Retina, inner ear
Tissue typeRecommended AAV serotypes
Smooth muscleAAV1, AAV2, AAV3, AAV5, AAV6, AAV7, AAV8, AAV9, AAVrh10
Skeletal muscleAAV1, AAV9
CNSAAV1, AAV2, AAV4, AAV5, AAV7, AAV8, AAV9, AAVrh10, AAV-PHP.eB
PNSAAV-PHP.S
BrainAAV1, AAV2, AAV5, AAV7, AAV8, AAV-DJ/8
RetinaAAV1, AAV2, AAV4, AAV5, AAV7, AAV8, AAV9, AAVrh10, AAV2-QuadYF, AAV2.7m8
Inner earAAV1, AAV2, AAV6.2, AAV8, AAV9, AAV2.7m8
LungAAV1, AAV3, AAV4, AAV5, AAV6, AAV6.2, AAV9, AAVrh10
LiverAAV1, AAV2, AAV3, AAV6, AAV6.2, AAV7, AAV8, AAV9, AAVrh10, AAV-DJ, AAV-DJ/8
PancreasAAV1, AAV2, AAV6, AAV8, AAV9, AAVrh10
HeartAAV1,AAV4, AAV5, AAV6, AAV8, AAV9, AAVrh10, AAV-DJ
KidneyAAV2, AAV4, AAV8, AAV9, AAVrh10, AAV-DJ, AAV-DJ/8
AdiposeAAV6, AAV8, AAV9
TestesAAV2, AAV9
SpleenAAV-DJ, AAV-DJ/8
Spinal nervesAAV2-retro
Endothelial cellsAAV2-QuadYF
Experimental validation

We have developed a number of proprietary techniques to optimize our triple transfection-based AAV packaging protocol and our virus has been validated to exhibit high transduction efficiency in mammalian cells.

Documents
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