Testimonials

VectorBuilder is proud to share our client's testimonials:

  • My lab has been using VectorBuilder services for the design of viral vectors and production of AAV2 viral particles for 6 years. VectorBuilder contributed to the success of our work, by enabling publication of multiple research articles which unitized these products.

    Feliks Trakhtenberg

    University of Connecticut School of Medicine, USA

  • I have been working with VectorBuilder from the beginning of my postdoc. I’ve chosen VectorBuilder because of the online platform to create customized vectors. It is really impressive how easy is to digitally clone and the order process. Specifically, in our paper, we’ve used VectorBuilder for several critical experiments, including the downregulation of Bassoon protein (AAV-shRNA) and to study the role of Bassoon in the propagation of tau (AAV-GFP-P2A-hTau). These experiments were extremely important to probe our hypothesis.

    Dr. Pablo A. Martínez

    Indiana University School of Medicine, USA

  • We chose to source AAV vectors from VectorBuilder due to the ease of online vector design, provision of expert technical support and vector production methods, which ensure high titer, purity and potency.

    Dr. George Mcilroy

    University of Aberdeen, Aberdeen, UK

  • Vectorbuilder is timely, communicative, easy to work with, and—most importantly—skilled at producing very high-quality viruses. We have found that the optimal multiplicity of infection for gene-editing experiments using an AAV6 donor supplied by VectorBuilder is lower than other published reports, suggesting that the provided virus is consistently of very high potency. Knowing this saves money and time.

    Dr. Brock Roberts

    Allen Institute for Brain Research

  • Our lab has been using VectorBuilder to aid in the design and synthesis of plasmids for the last 3-4 years. They have always been extremely helpful with design requests. I would highly recommend VectorBuilder's services to anyone wanting to accelerate their research progress.

    Dr. Andrew Cox

    Peter MacCallum Cancer Centre

  • We have used VectorBuilder's services for plasmid desisgn as well as AAV and Lentiviral vector production for more than four years years. Their service is consistent, reliable, and prompt. A major plus is the technical support team.

    Dr. Kevin Watt

    Murdoch Children's Research Institute, Victoria, Australia

  • Amazing experience cooperating with VectorBuilder! As one of the most popular technologies, mRNA vaccine technology has been used extensively to develop therapeutics and vaccines targeting various infectious and non-infectious diseases. Recently, Daniel Meng’s R&D team at VectorBuilder rapidly established the IVT mRNA CRO platform, and we closely collaborated on developing mRNA-based HIV and influenza vaccines. I am so impressed by their scientific vision as well as their problem solving capabilities. Once again, VectorBuilder is trustworthy.

    Dr. Caijun Sun

    Sun Yat-sen University, Guangzhou, China

  • It has been a great experience working with VB. I was one of the first to test their empty capsid (AAV VLP) service. For my batch capsids were nearly completely empty (>97% by AUC and TEM) and were thus a huge help for assay development as a negative control. I also want to highlight that my contact Matthew Wheeler was always very helpful and transparent for any queries I had and that the team is very agile. A big plus is also the very intuitive website. Overall, a big thumbs up for VectorBuilder from my side.

    Dr. Andrei Hutanu

    Analytical Biochemist/QC Method Development, Roche, Switzerland

  • I am very satisfied with the constructions (double mutants) made by Vectorbuilder. The service is very serious and rigorous. They started by verifying our plasmid by doing digestions. As this was not enough, they even sequenced part of the plasmid, which then ensured the success of the constructs. Constant email exchanges for verification steps were ensured with almost instantaneous responsiveness! Quite impressive! Once the sequence was clarified, production was not long, and we were able to do our experiments under excellent conditions. The results were as good as we expected. Bravo again and thank you for the efficiency, the rigor, and the seriousness.

    Sabine Levi

    French National Centre for Scientific Research (CNRS), France

  • After ordering two plasmids from VectorBuilder, I would like to give you some feedback on the products. I am very satisfied with the plasmids sent. It's a real time saver and moreover, I had no problems concerning the expression of these plasmids in the target cells (human). The team's customer service is also attentive to requests and is very reactive regarding any problems encountered with the plasmids.

    Morgane Baldaccini

    French National Centre for Scientific Research (CNRS), France

  • We have used VectorBuilder services for one year. The website is very user-friendly for designing vectors, and there are automatic verifications that make pertinent suggestions when validating sequences. The flexibility of the service is truly amazing, and we can get a perfectly characterized plasmid for the price of an antibody. Working with VectorBuilder saves us a great deal of time, and time is money. We just can't wait to get the plasmid when ordered!

    Nous utilisons les services de VectorBuilder depuis un an. Le site web est très simple à utiliser concernant le design de vecteurs et comprend des vérifications automatiques qui font des suggestions pertinentes lors de la validation des séquences. La flexibilité du service est vraiment impressionnante et nous pouvons obtenir un plasmide parfaitement caractérisé pour le prix d'un anticorps. Travailler avec VectorBuilder nous fait gagner beaucoup de temps, et le temps, c'est de l'argent. Nous sommes impatients de recevoir le plasmide lorsque nous le commandons !

    Fabien Chevalier

    French National Centre for Scientific Research (CNRS), France

  • Before using VectorBuilder, producing lentiviral overexpression vectors for our in vitro models was a recurring and time-consuming enterprise. We faced frequent challenges in every experimental step, which made it difficult to plan the actual experiments. Setting up the process with VectorBuilder was very efficient, thanks to their great expertise and support. Once we started using VectorBuilder's services, we noticed a substantial increase in productivity as well as a more uniform and predictable transduction pattern of our target cells.

    We went on to use VectorBuilder for a large-scale CRISPR screen with a custom library spanning over 1,600 genes. With the provided virus, we were able to easily maintain cell representation and we derived a number of interesting hit genes, which we are excited to follow up on. A big thank you to the entire VectorBuilder Team!

    Dominic Schmid

    University of Basel

  • To me, VectorBuilder specially Stella Gideon has been amazing! We have gotten our plasmids made by them for over 3 years and every time they are on point! The plasmids arrive on time, and their support team is always on standby for any help I may need!

    Nemil Bhatt

    University of Texas Medical Branch

  • VectorBuilder's AAV serotype panel helped us to choose the best expressing serotype for our brain area and animal model of interest. By piloting injections using a selection of serotypes provided in the panel, we obtained invaluable pilot data before ordering a large scale virus production. This allowed us to save a considerable amount of time and resources. The technical support was great, they answer our questions promptly provided great advice. VectorBuilder technical support team further help us designing and cloning our AAV viral vector. Our preliminary data allowed to map retrogradely labelled neurons in the amygdala using light-sheet microscopy. We would not hesitate to use VectorBuilder for our next projects

    Dr. Cristina Martinez Gonzalez

    University of Edinburgh, United Kingdom

  • To me, science is not a profession. It's a passion for the unknown. It's a constant celebration of perseverance, and love for the truth. And with the professional support of VectorBuilder, the superior quality of VectorBuilder products, and the very competent personalized assistance (thank you so much, Dr. Mirus), science also became an ever-growing source of joy to me. Why? Because since I started to rely on VectorBuilder, I have significantly increased my research output and realized more complex projects much more quickly, giving me time needed to truly enjoy what I'm doing.

    Ricardo Koch

    Institute of Oncology Research (IOR), Switzerland

  • We have used VectorBuilder services for 3 years now. In our lab, we generate different types of vectors, with custom promoters and difficult sequences. I often had trouble when I synthesized these custom elements elsewhere. Going back to cloning by ourselves is no longer our first option since they are fast, efficient and their prices are more than fair. On top of that, their customer service is unbeatable.

    Dr. Gaetano Gargiulo

    Max-Delbrück-Center for Molecular Medicine (MDC), Germany

  • VectorBuilder makes the process of designing and building vectors easy and efficient. Justin Mirus, as the lead, and the unique and energetic design team are promptly ready to help and support our request and advise us with their great expertise and effective services.

    Denise Panella

    Glycostem, Netherlands

  • VectorBuilder has been the partner every researcher would wish for during those dark moments of paper revision. They offer the complete package: a wide range of viral vector design options, an extremely user-friendly and intuitive website, direct communication, personal and knowledgeable step-by-step guidance throughout the designing and ordering process, accountability, amazing discounts, unique customer support, fast shipping, and guaranteed virus titers and efficiency. So here’s my big thumbs up for a great product and service provider who has proven indispensable in addressing my research questions!

    Evgenia Salta

    Katholieke Universiteit Leuven/VIB, Belgium

  • VectorBuilder is an amazing way to save time normally dedicated to molecular biology. The website is easy to use, well-conceived and allows quick design in most cases. For more unusual requests, the team is energetic, reply promptly on every request, and always finds a way to help. Low prices and short timelines really make it possible to consider VectorBuilder for many cloning matters.

    Laura Israel

    Novartis (Basel), Switzerland

  • VectorBuilder offers a comprehensive online platform for vector design. We found the options for customising our vectors to be extensive and the interface simple - allowing this to be done ‘with a click’. The team has been really responsive when I wanted to check things through - thanks Christina! In my view, VectorBuilder is a platform worth investigating when you are looking to save time and outsource cloning and virus production.

    Craig Southern

    LifeArc, UK

  • VectorBuilder has allowed us to rapidly iterate and expand our cell editing needs. Not only do they supply a service that allows easy, quality vectors to be delivered, but they have outstanding technical and customer support. The territory manager Alejandro Izurieta has gone above and beyond to get us information we need, or connect us to the right person. We will continue to use VectorBuilder in the future for all our vector needs.

    Alan Mardinly

    Director of Biology, Science (California), USA

  • We ordered adenovirus from Vectorbuilder company to overexpress our interest genes. The service is fast, considerate, and high-qualified. Besides, the adenovirus is very effective to overexpress our target gene.

    Wanbao Yang

    Texas A&M University, USA

  • We find that it is more efficacious to use VectorBuilder for our molecular biology needs. Purchasing vectors and making use of cloning services frees our students’ time to concentrate on experiments. We have worked with VectorBuilder for quite some time and are always pleased with the efficient, effective, and affordable service. In particular, we are impressed by, and appreciate, how well our rep., Justin Mirus, takes care of our needs. VectorBuilder contributes money and expertise to the advancement of science, for example, to enable conferences at our institute.

    Jared Sterneckert's lab

    Center for Regenerative Therapies, Germany

  • Two years ago, when another CRO was struggling to provide the requested clones that I ordered, I reached out to Vector Builder to try to make them for me. Very difficult clones to make due to many repeats of amino acids. They got the clones done for me with no issues and very quickly. I have been using Vector Builder since then. Along with this, working with Alejandro Izurieta (California Territory Manager) has been great. He is knowledgeable, and professional promptly answers my request and is courteous. I rely on him to get the service that I need from Vector Builder done. He is just wonderful!

    Irena Levin

    Takeda (California), USA

  • Vectorbuilder's service is extremely consistent. We started with regular plasmids and AAV plasmids. Since last year we have ordered many piggybac plasmids. All worked as expected.

    Shuhsien Sheu

    Janelia Research Campus, USA

  • We began using VectorBuilder for adenoviral plasmids construction about a year ago. We normally handled all cloning projects ourselves, but when it comes to adenoviral vectors, because of their large size, sometimes the cloning is kind of tricky for inexperienced students. So we decided to give VectorBuilder a try. The plasmid arrived one month later and the virus we generated from it expressed the correct gene. We have ordered more than 10 adenoviral plasmids and they all work fine. VectorBuilder has saved us a lot of time.

    Lei Sun

    DUKE-NUS Graduate Medical School, Singapore

  • I recently met one of the VectorBuilder product specialist during AAI-2019 meeting at San Diego Convention Center. Her name is Dr. Swati Roy, and she provided me excellent information regarding their products and vector designs. And then, we recently purchased CRISPR Cas9 dual gRNA vector. Now, we are ordering few more plasmid vectors. As per my experiences, VectorBuilder customer services are excellent and user-friendly. The VectorBuilder product prices are reasonable.

    Shahanshah Khan

    UT Southwestern Medical Center, USA

  • The VectorBuilder's system for new vector design is easy to be used, checked and analyzed. I like the system very much and highly recommend the system to new customers. I am going to use their service for multiple projects again.

    Ke-He Ruan

    University of Houston, USA

  • We have ordered quite a few plasmids from VectorBuilder in the past 1-2 years, ranging from regular expression plasmids, PiggyBac, and AAV vectors. The products have been delivered on time and worked as expected. Highly recommend!

    Shu-Hsien Sheu

    Janelia Research Campus of Howard Hughes Medical Institute, USA

  • VectorBuilder’s online vector design platform is user friendly and easy to use. All one needs to do is select sequences of inserts either from their vast data base or provide one’s own unique sequences. The sequence database provides a wealth of information and links to peer-reviewed publications. Placing order for the vectors is easy and their technical and supporting staff are prompt in offering solutions. They offer services at low prices in comparison to other commercial sources. With the guaranteed service, I can concentrate on my experiments instead of constructing the vector. Thanks are especially due to Stella Gideon for her support at every step. If one is constrained with time and manpower to devote to vector construction, leave it to VectorBuilder. It is worth every penny.

    Bala Kolli

    Wayne State University, USA

  • We recently purchased a lentiviral plasmid for constitutive expression of GFP and luciferase and the corresponding lentivirus from VectorBuilder. We have infected several tumor cell lines with excellent results. The lentiviral stocks worked very nicely, saving us a lot of time. We are now using these modified cell lines for monitoring tumor growth in animal models using in vivo imaging.

    Javier Alonso

    Research institute for Rare Diseases, Spain

  • The website design is super user-friendly and easy to follow, I'm really impressed. It was great working with Swati Roy and Stella Gideon. Shout out to the customer support - they have always been responsive and extremely helpful.

    Jaya Pratap Pinnamaneni

    Baylor College of Medicine, USA

  • I would like to share with you my positive experience purchasing a lentiviral vector from VectorBuilder.
     
    First, I am an immunologist and have little experience with cloning. In my recent project, we needed to overexpress a gene in melanoma cells. The transfection efficiency using plasmids in this cell line was extremely low, less than 10%. Then, we tried to use antibiotics to select transfected cells and establish stably expressing cell lines. Unfortunately, for unknown reasons, the ‘stable transfectants’ lost the transfected genes! I was frustrated at this point, until I met VectorBuilder at the EACR conference in Amsterdam. Using the user-friendly website from VectorBuilder, I designed for the first time in my life my own lentiviral vector and got the product in less than one month. My group leader, who has a lot of cloning experience, could not believe that it worked so quickly. Of course, most important are the experimental results. To access the transduction efficiency, I included an EGFP tag in the vector. Now, I can transduce the melanoma cells with nearly 100% positivity. In the following assays, the genes transduced into the tumor cells resulted in a 6-fold induction in tumor immunogenicity.
    Taken together, I strongly recommend VectorBuilder to researchers, especially those who are not so experienced with cloning, and am convinced that the service and product quality from VectorBuilder can further help me to proceed with my own projects more effectively.

    Fang Zhao

    UK-Essen, Germany

  • I have been consistently using VectorBuilder over the last couple of years and have been extremely satisfied with the vectors they've delivered. These include standard over-expression vectors, and Cas9 vectors on the PiggyBac backbone. All of the vectors I have purchased so far have passed my QC and provided reproducible functional data.

    Daksh Thaper

    Vancouver Prostate Centre, Canada

  • VectorBuilder is the best platform for designing and synthesizing constructs. The online vector design tool is so convenient and fun to use. Ms. Joyce Zhang is always very professional in replying my questions and does it so timely. One time, something did not work out, the refund process was smooth and all the staff were very nice and professional. The constructs I asked for had two fluorescent proteins with two promotors, one has NLS and the other does not. I did transfection and it worked out very well. Joyce helped me all through this process, making my life very easy. Basically, I had many pleasant experiences with Vectorbuilder, and I strongly recommend using it. I had tried other companies, they turned out to be either slow or lacked strong technical support or are expensive. Vectorbuilder is not the cheapest, but considering the quality of its service, it is worth the money.

    Shanshan Zhu

    Johns Hopkins University, USA

  • Amazing experience with VectorBuilder! I ordered plasmids at the beginning of December, and despite the holiday season they arrived exactly as scheduled. Furthermore, customer support reached out to ask what would be my desired delivery date, considering potential holiday conflicts at my institute. Overall, I am very satisfied, would highly recommend VectorBuilder (which I already do) and will buy from them again. All the best from my side!

    Tamara Tomin

    Medical University of Graz, Austria

  • VectorBuilder is efficient and easy to use for the outsourcing of plasmid generations. We used the PiggBac system for transgenic modifications and we have had great success.

    Luis Cruz

    University of California San Diego, USA

  • Using VectorBuilder has transformed the way I do my research. VectorBuilder’s easy-to-use web interface allows me to custom build my own plasmids, freeing up time that I would have wasted cloning and instead letting me focus on the research that really matters. They offer great prices with a fast turnaround time. Their friendly and well-informed technical support team are on hand every step of the way, should you need any help. I would strongly recommend VectorBuilder to anyone wanting to accelerate the progress of their research.

    David M Brown

    University of Nottingham, UK

  • We recently purchased a number of customized plasmids on VectorBuilder. They consisted of expression vectors of two or more ORFs. Our experience was satisfactory, efficient and cost-effective.

    The designing stage was facilitated by the VectorBuilder design tool online, in which many of the most commonly used sequences, like fluorescent proteins, selection drug resistance genes, and fusion or cleavage linkers were readily offered to be picked from the ORF lists. Regarding our specific sequences, once pasted into the corresponding window of the design tool, they were immediately matched with the International Nucleotide Sequence Database Collaboration (DDBJ/EMBL/GenBank) accession numbers and compared to the databases. Where there were discrepancies, we were asked which transcripts/cDNAs we preferred to use.

    After completing the order, in a matter of three to four weeks, we had in our hands 8 of the 10 plasmids, as offered. For the rest of the plasmids we provided the template for a specific sequence. Before proceeding with the cloning, they sequenced it, detected a mutation and corrected it at a low extra cost upon our approval. A week later these corrected plasmids were delivered as well. During the whole process, VectorBuilder’s European team kept a personalized email dialogue with us, enabling us to follow-up and make decisions or accept suggestions about the plasmids.

    We have satisfactorily tested every plasmid now. We think that our decision to work with VectorBuilder has boosted our research by saving us time, resources and enabling us to concentrate more on our experiments.

    Leonel Munoz

    The Karlsruhe institute of Technology, Germany

  • I have been using VectorBuilder's cloning service for several years and am extremely happy with their products and services. Their online design software is very easy to use and provides the most cutting edge solutions for today's most demanding molecular cloning needs. Their customer service is outstanding, with great technical competence and the delivery of my vectors was on time. I have saved months of precious research time by using VectorBuilder. I would recommend VectorBuilder to anyone who wants the best vector design and molecular cloning services.

    Guohao Dai

    Northeastern University, USA

  • VectorBuilder’s service makes designing vectors incredibly simple and there’s no software required, it can all be done through their easy to use website. The lentivirus production service is a massive bonus as it’s so quick and reasonably priced. There’s really no reason to make our own lentivirus anymore. Thanks for a fantastic service.

    Timothy London

    TC Biopharm, UK

  • Our laboratory has had great success with both viral constructs and ready-to-use virus prepared by VectorBuilder. They provide excellent customer service at a reasonable price, and their website is extremely user-friendly. Very pleased we discovered this company.

    Marco Seandel

    Weill Cornell Medical College, USA

  • We requested a custom AAV (4-YF) from VectorBuilder. The ordering process was easy and the customer support team were really helpful. Our virus had the expected titer and worked extremely well for our chosen application.

    Jessica Rodgers

    The University of Manchester, UK

  • We were searching for a way to outsource the cloning of our vectors and we are very happy with the service. The versatility of the tools make it a great time saver and the vectors were delivered on-time. Lentiviruses packaged are also very reliable and have been very successful in transduction studies in primary cells. Great customer service with fast response times and the team at VectorBuilder are very knowledgeable.

    Kathy Ngo

    Aridis Pharmaceuticals, USA

  • I have been successfully cloning for 3+ years, but the last time we tried generating KO cell lines with CRISPR/Cas9 vectors, we were not successful. This was upsetting, especially since I invested a lot of time in these efforts. Then, by chance, I attended one of VectorBuilder's seminars. Due to their reasonable prices and the fact I could focus my time on other work, we decided to give their lentiviral (dual gRNA) Cas9 plasmid a try. The turnaround time was reasonable, their customer service was excellent and they actually gave feedback about the status of my cloning projects. Their website is also easy to use. But most importantly: using their Cas9 dual gRNA vector, I was able to successfully generate KO cell lines! 

    I recommend VectorBuilder to CRISPRCas9 users without any hesitation. 

    Anna Salamero Boix

    Georg-Speyer-Haus Frankfurt, Germany

  • We have ordered several vectors from VectorBuilder, including regular expression plasmids, targeting vectors and AAVs. The constructs were delivered on time and worked very well. They saved us a lot of time and money. Their design team pays attention to detail and made very useful suggestions. Their customer service is excellent as well. Overall, we highly recommend VectorBuilder.

    Martin Riccomagno

    University of California, Riverside, USA

  • We applied CRISPR cas9 nickase in our study. VectorBuilder helped us design the gRNAs and construct plasmids with double gRNAs and double selection markers (EGFP/puromycin). The gRNA specificity is quite good, better than available online design tools. We got the knockout cell lines in the first run of electroporation with no off-target events. I would recommend VectorBuilder to CRISPR cas9 users.

    Jie Qin

    University Clinic Cologne, Germany

  • I ordered a plasmid vector two years ago. The design on the web platform was easy. Contacts with the specialists to arrange further details were excellent: they were very supportive!

    Emanuela Signori

    Consiglio Nazionale delle Ricerche, Italy

  • I’ve always been using VectorBuilder’s vector construction service. I’m generally satisfied with the quality of its service, and the problems I encountered were solved quickly.

    Huimin Chen

    The First Affiliated Hospital, Sun Yat-sen University, China

  • We rely on VectorBuilder for high-quality molecular biology reagents. The constructs and particles are delivered to us in a timely manner. They have been more than willing to generate new plasmids and viruses if they do not work well. In summary, we have been very satisfied with VectorBuilder.

    Sathish Srinivasan

    Oklahoma Medical Research Foundation, USA

Tell us your experience with VectorBuilder!

user_testimonials

User Testimonials




Download pdf
Design My Vector Send Design Request