VectorBuilder's AAV serotype panel helped us to choose the best expressing serotype for our brain area and animal model of interest. By piloting injections using a selection of serotypes provided in the panel, we obtained invaluable pilot data before ordering a large scale virus production. This allowed us to save a considerable amount of time and resources. The technical support was great, they answer our questions promptly provided great advice. VectorBuilder technical support team further help us designing and cloning our AAV viral vector. Our preliminary data allowed to map retrogradely labelled neurons in the amygdala using light-sheet microscopy. We would not hesitate to use VectorBuilder for our next projects
Dr. Cristina Martinez Gonzalez
University of Edinburgh, United Kingdom
To me, science is not a profession. It's a passion for the unknown. It's a constant celebration of perseverance, and love for the truth. And with the professional support of VectorBuilder, the superior quality of VectorBuilder products, and the very competent personalized assistance (thank you so much, Dr. Mirus), science also became an ever-growing source of joy to me. Why? Because since I started to rely on VectorBuilder, I have significantly increased my research output and realized more complex projects much more quickly, giving me time needed to truly enjoy what I'm doing.
Institute of Oncology Research (IOR), Switzerland
We have used VectorBuilder services for 3 years now. In our lab, we generate different types of vectors, with custom promoters and difficult sequences. I often had trouble when I synthesized these custom elements elsewhere. Going back to cloning by ourselves is no longer our first option since they are fast, efficient and their prices are more than fair. On top of that, their customer service is unbeatable.
Dr. Gaetano Gargiulo
Max-Delbrück-Center for Molecular Medicine (MDC), Germany
VectorBuilder makes the process of designing and building vectors easy and efficient. Justin Mirus, as the lead, and the unique and energetic design team are promptly ready to help and support our request and advise us with their great expertise and effective services.
VectorBuilder has been the partner every researcher would wish for during those dark moments of paper revision. They offer the complete package: a wide range of viral vector design options, an extremely user-friendly and intuitive website, direct communication, personal and knowledgeable step-by-step guidance throughout the designing and ordering process, accountability, amazing discounts, unique customer support, fast shipping, and guaranteed virus titers and efficiency. So here’s my big thumbs up for a great product and service provider who has proven indispensable in addressing my research questions!
Katholieke Universiteit Leuven/VIB, Belgium
VectorBuilder is an amazing way to save time normally dedicated to molecular biology. The website is easy to use, well-conceived and allows quick design in most cases. For more unusual requests, the team is energetic, reply promptly on every request, and always finds a way to help. Low prices and short timelines really make it possible to consider VectorBuilder for many cloning matters.
Novartis (Basel), Switzerland
VectorBuilder offers a comprehensive online platform for vector design. We found the options for customising our vectors to be extensive and the interface simple - allowing this to be done ‘with a click’. The team has been really responsive when I wanted to check things through - thanks Christina! In my view, VectorBuilder is a platform worth investigating when you are looking to save time and outsource cloning and virus production.
We ordered adenovirus from Vectorbuilder company to overexpress our interest genes. The service is fast, considerate, and high-qualified. Besides, the adenovirus is very effective to overexpress our target gene.
Texas A&M University, USA
We find that it is more efficacious to use VectorBuilder for our molecular biology needs. Purchasing vectors and making use of cloning services frees our students’ time to concentrate on experiments. We have worked with VectorBuilder for quite some time and are always pleased with the efficient, effective, and affordable service. In particular, we are impressed by, and appreciate, how well our rep., Justin Mirus, takes care of our needs. VectorBuilder contributes money and expertise to the advancement of science, for example, to enable conferences at our institute.
Jared Sterneckert's lab
Center for Regenerative Therapies, Germany
Vectorbuilder's service is extremely consistent. We started with regular plasmids and AAV plasmids. Since last year we have ordered many piggybac plasmids. All worked as expected.
Janelia Research Campus, USA
We began using VectorBuilder for adenoviral plasmids construction about a year ago. We normally handled all cloning projects ourselves, but when it comes to adenoviral vectors, because of their large size, sometimes the cloning is kind of tricky for inexperienced students. So we decided to give VectorBuilder a try. The plasmid arrived one month later and the virus we generated from it expressed the correct gene. We have ordered more than 10 adenoviral plasmids and they all work fine. VectorBuilder has saved us a lot of time.
DUKE-NUS Graduate Medical School, Singapore
I recently met one of the VectorBuilder product specialist during AAI-2019 meeting at San Diego Convention Center. Her name is Dr. Swati Roy, and she provided me excellent information regarding their products and vector designs. And then, we recently purchased CRISPR Cas9 dual gRNA vector. Now, we are ordering few more plasmid vectors. As per my experiences, VectorBuilder customer services are excellent and user-friendly. The VectorBuilder product prices are reasonable.
UT Southwestern Medical Center, USA
The VectorBuilder's system for new vector design is easy to be used, checked and analyzed. I like the system very much and highly recommend the system to new customers. I am going to use their service for multiple projects again.
University of Houston, USA
We have ordered quite a few plasmids from VectorBuilder in the past 1-2 years, ranging from regular expression plasmids, P
iggyBac, and AAV vectors. The products have been delivered on time and worked as expected. Highly recommend!
Janelia Research Campus of Howard Hughes Medical Institute, USA
VectorBuilder’s online vector design platform is user friendly and easy to use. All one needs to do is select sequences of inserts either from their vast data base or provide one’s own unique sequences. The sequence database provides a wealth of information and links to peer-reviewed publications. Placing order for the vectors is easy and their technical and supporting staff are prompt in offering solutions. They offer services at low prices in comparison to other commercial sources. With the guaranteed service, I can concentrate on my experiments instead of constructing the vector. Thanks are especially due to Stella Gideon for her support at every step. If one is constrained with time and manpower to devote to vector construction, leave it to VectorBuilder. It is worth every penny.
Wayne State University, USA
We recently purchased a lentiviral plasmid for constitutive expression of GFP and luciferase and the corresponding lentivirus from VectorBuilder. We have infected several tumor cell lines with excellent results. The lentiviral stocks worked very nicely, saving us a lot of time. We are now using these modified cell lines for monitoring tumor growth in animal models using in vivo imaging.
Research institute for Rare Diseases, Spain
The website design is super user-friendly and easy to follow, I'm really impressed. It was great working with Swati Roy and Stella Gideon. Shout out to the customer support - they have always been responsive and extremely helpful.
Jaya Pratap Pinnamaneni
Baylor College of Medicine, USA
I would like to share with you my positive experience purchasing a lentiviral vector from VectorBuilder.
First, I am an immunologist and have little experience with cloning. In my recent project, we needed to overexpress a gene in melanoma cells. The transfection efficiency using plasmids in this cell line was extremely low, less than 10%. Then, we tried to use antibiotics to select transfected cells and establish stably expressing cell lines. Unfortunately, for unknown reasons, the ‘stable transfectants’ lost the transfected genes! I was frustrated at this
point,until I met VectorBuilder at the EACR conference in Amsterdam. Using the user-friendly website from VectorBuilder, I designed for the first time in my life my own lentiviral vector and got the product in less than one month. My group leader, who has a lot of cloning experience, could not believe that it worked so quickly. Of course, most important are the experimental results. To access the transduction efficiency, I included an EGFP tag in the vector. Now, I can transduce the melanoma cells with nearly 100% positivity. In the following assays, the genes transduced into the tumor cells resulted in a 6-fold induction in tumor immunogenicity.
Taken together, I strongly recommend VectorBuilder to researchers, especially those who are not so experienced with
cloning,and am convinced that the service and product quality from VectorBuilder can further help me to proceed with my own projects more effectively.
I have been consistently using VectorBuilder over the last couple of years and have been extremely satisfied with the vectors they've delivered. These include standard over-expression vectors, and Cas9 vectors on the PiggyBac backbone. All of the vectors I have purchased so far have passed my QC and provided reproducible functional data.
Vancouver Prostate Centre, Canada
VectorBuilder is the best platform for designing and synthesizing constructs. The online vector design tool is so convenient and fun to use. Ms. Joyce Zhang is always very professional in replying my questions and does it so timely. One time, something did not work out, the refund process was smooth and all the staff were very nice and professional. The constructs I asked for had two fluorescent proteins with two promotors, one has NLS and the other does not. I did transfection and it worked out very well. Joyce helped me all through this process, making my life very easy. Basically, I had many pleasant experiences with Vectorbuilder, and I strongly recommend using it. I had tried other companies, they turned out to be either slow or lacked strong technical support or are expensive. Vectorbuilder is not the cheapest, but considering the quality of its service, it is worth the money.
Johns Hopkins University, USA
Amazing experience with VectorBuilder! I ordered plasmids at the beginning of December, and despite the holiday
seasonthey arrived exactly as scheduled. Furthermore, customer support reached out to ask what would be my desired delivery date, considering potential holiday conflicts at my institute. Overall, I am very satisfied, would highly recommend VectorBuilder (which I already do) and will buy from them again. All the best from my side!
Medical University of Graz, Austria
VectorBuilder is efficient and easy to use for the outsourcing of plasmid generations. We used the PiggBac system for transgenic modifications and we have had great success.
University of California San Diego, USA
Using VectorBuilder has transformed the way I do my research. VectorBuilder’s easy-to-use web interface allows me to custom build my own plasmids, freeing up time that I would have wasted cloning and instead letting me focus on the research that really matters. They offer great prices with a fast turnaround time. Their friendly and well-informed technical support team are on hand every step of the way, should you need any help. I would strongly recommend VectorBuilder to anyone wanting to accelerate the progress of their research.
David M Brown
University of Nottingham, UK
We recently purchased a number of customized plasmids on VectorBuilder. They consisted of expression vectors of two or more ORFs. Our experience was satisfactory, efficient and cost-effective.
The designing stage was facilitated by the VectorBuilder design tool online, in which many of the most commonly used sequences, like fluorescent proteins, selection drug resistance genes, and fusion or cleavage linkers were readily offered to be picked from the ORF lists. Regarding our specific sequences, once pasted into the corresponding window of the design tool, they were immediately matched with the International Nucleotide Sequence Database Collaboration (DDBJ/EMBL/GenBank) accession numbers and compared to the databases. Where there were discrepancies, we were asked which transcripts/cDNAs we preferred to use.
After completing the order, in a matter of three to four weeks, we had in our hands 8 of the 10 plasmids, as offered. For the rest of the
plasmidswe provided the template for a specific sequence. Before proceeding with the cloning, they sequenced it, detected a mutation and corrected it at a low extra cost upon our approval. A week later these corrected plasmids were delivered as well. During the whole process, VectorBuilder’s European team kept a personalized email dialogue with us, enabling us to follow-up and make decisions or accept suggestions about the plasmids.
We have satisfactorily tested every plasmid now. We think that our decision to work with VectorBuilder has boosted our research by saving us time, resources and enabling us to concentrate more on our experiments.
instituteof Technology, Germany
I have been using VectorBuilder's cloning service for several years and am extremely happy with their products and services. Their online design software is very easy to use and provides the most cutting edge solutions for today's most demanding molecular cloning needs. Their customer service is outstanding, with great technical competence and the delivery of my vectors was on time. I have saved months of precious research time by using VectorBuilder. I would recommend VectorBuilder to anyone who wants the best vector design and molecular cloning services.
Northeastern University, USA
VectorBuilder’s service makes designing vectors incredibly simple and there’s no software required, it can all be done through their easy to use website. The lentivirus production service is a massive bonus as it’s so quick and reasonably priced. There’s really no reason to make our own lentivirus anymore. Thanks for a fantastic service.
TC Biopharm, UK
Our laboratory has had great success with both viral constructs and ready-to-use virus prepared by VectorBuilder. They provide excellent customer service at a reasonable price, and their website is extremely user-friendly. Very pleased we discovered this company.
Weill Cornell Medical College, USA
We requested a custom AAV (4-YF) from VectorBuilder. The ordering process was easy and the customer support team were really helpful. Our virus had the expected titer and worked extremely well for our chosen application.
The University of Manchester, UK
We were searching for a way to outsource the cloning of our vectors and we are very happy with the service. The versatility of the tools make it a great time saver and the vectors were delivered on-time. Lentiviruses packaged are also very reliable and have been very successful in transduction studies in primary cells. Great customer service with fast response times and the team at VectorBuilder are very knowledgeable.
Aridis Pharmaceuticals, USA
I have been successfully cloning for 3+ years, but the last time we tried generating KO cell lines with CRISPR/Cas9 vectors, we were not successful. This was upsetting, especially since I invested a lot of time in these efforts. Then, by chance, I attended one of VectorBuilder's seminars. Due to their reasonable prices and the fact I could focus my time on other work, we decided to give their lentiviral (dual gRNA) Cas9 plasmid a try. The turnaround time was reasonable, their customer service was excellent and they actually gave feedback about the status of my cloning projects. Their website is also easy to use. But most importantly: using their Cas9 dual
gRNAvector, I was able to successfully generate KO cell lines!
I recommend VectorBuilder to CRISPRCas9 users without any hesitation.
Anna Salamero Boix
Georg-Speyer-Haus Frankfurt, Germany
We have ordered several vectors from VectorBuilder, including regular expression plasmids, targeting vectors and AAVs. The constructs were delivered on time and worked very well. They saved us a lot of time and money. Their design team pays attention to detail and made very useful suggestions. Their customer service is excellent as well. Overall, we highly recommend VectorBuilder.
University of California, Riverside, USA
CRISPRcas9 nickase in our study. VectorBuilder helped us design the gRNAs and construct plasmids with double gRNAs and double selection markers (EGFP/puromycin). The gRNA specificity is quite good, better than available online design tools. We got the knockout cell lines in the first run of electroporation with no off-target events. I would recommend VectorBuilder to CRISPR cas9 users.
University Clinic Cologne, Germany
I ordered a plasmid vector two years ago. The design on the web platform was easy. Contacts with the specialists to arrange further details were excellent: they were very supportive!
Consiglio Nazionale delle Ricerche, Italy
I’ve always been using VectorBuilder’s vector construction service. I’m generally satisfied with the quality of its service, and the problems I encountered were solved quickly.
The First Affiliated Hospital, Sun Yat-sen University, China
We rely on VectorBuilder for high-quality molecular biology reagents. The constructs and particles are delivered to us in a timely manner. They have been more than willing to generate new plasmids and viruses if they do not work well. In summary, we have been very satisfied with VectorBuilder.
Oklahoma Medical Research Foundation, USA
The services provided by VectorBuilder are excellent. I had a few vector constructs made by the VectorBuilder. The description of the constructs by their software is excellent. The technical team of the company seems to have a sound knowledge and the technical suggestion regarding vector construction given to me by them were excellent.
Quaild-i-Azame University, Pakistan
I’ve used VectorBuilder’s lentivirus packaging service. The virus titer was as promised. Strong fluorescence was observed after transduction. The gene knockdown efficiency was also satisfactory.
Southern Medical University, China
VectorBuilder is an amazing way to save my time for the generation of my plasmid and provide excellent downstream services. You can get any custom designed vectors you want at a very good price. I am sure I will order the vectors I need in the near future.
Houston Methodist Research Institute, USA
I purchased a lentivirus vector and received it in less than two weeks. I am so glad that I found VectorBuilder, so I can design my lentiviral vector by picking any promotor, and from a variety of antibiotic selection markers and fluorescent tags. Upon transducing several cell lines, my target genes were highly and stably expressed with expression persisting for at least after one month after undergoing selection.
H. Lee Moffitt Cancer Center & Research Institute, USA
VectorBuilder is an intuitive tool for developing vectors at affordable prices. I was in need of a CRISPR lentiviral system with a Puro selection cassette due to working with delicate cells that cannot handle FACS sorting pressures. With their online interface, it was easy to construct the vector I wanted and received the vector in a timely manner! It was simple to produce virus and test it in our cell culture and generated clonal cells with good gene knockdown. I definitely will be ordering more vectors from VectorBuilder to finish up my thesis!
University of Colorado, USA
VectorBuilder has provided very satisfactory service in making various AAV vector constructs for us. The new sales representative is very proactive in offering help on explaining and introducing other VectorBuilder service to us. Expecting to get more support and service from VectorBuilder.
University of Texas Medical School at Ho, USA
Thumbs up! I chose VectorBuilder after comparing it with other companies, because it provides high-quality products and services at unbeatable prices.
The First Affiliated Hospital of Guangzhou Medical University, China
I’ve used VectorBuilder’s vector construction and lentivirus packaging services. The turnaround time was short and everything worked well. Also, I received
quickresponses from the customer support team.
Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, China
VectorBuilder has helped with my research progress. Cloning hasn't been an issue in our lab until recently. Their construct worked well. They saved me a lot of time and provide great services!
University of California, Riverside, USA
I would like to share with you all my experience with VectorBuilder. I do a lot of vector designs for different applications. VectorBuilder website is easy to use and offers efficient and fast vector design tools. In addition, VectorBuilder team is friendly and is open to accommodate special custom and bespoke requirements for vector modifications that are not readily available on the website. Finally, the price is more than reasonable, and I would recommend checking VectorBuilder website for cloning projects.
Wellcome Trust/CRUK Gurdon Institute, University of Cambridge, UK
We ordered vectors from Vectorbuilder already many times. We are happy with the service. The vectors work well and service is fast. I would highly recommend Vectorbuilder. We have very good experience with them.
University of Florida, USA
I used the lentivirus packaging service from VectorBuilder, and the process is very easy. All I needed to do is to ship a small quantity of transfer plasmid, and VectorBuilder will do the rest. Then I received the high titer virus at the end.
UT MD Anderson Cancer Center, USA
The scientific standards of VectorBuilder’s services are unquestionably high. The web interface to design vectors is intuitive and in fact so easy to use that it can even serve for educational purposes. VectorBuilder has pretty much covered everything one can think about when wanting to design an expression vector, it’s great to have all the versatility on one page. Turnaround time is fast and owing to their professionalism and customer friendly attitude I can recommend VectorBuilder’s services without
Max Planck Institute for Heart and Lung Research, Germany
We ordered adenovirus from VectorBuilder to overexpress our protein of interest in primary mouse hepatocytes. The ordering process, including vector design, was very fast and easy. We received the plasmid and adenovirus within the time frame that was stated during the ordering process and the transduction efficiency of the adenovirus was very high, enabling us to use a low MOI. As a bonus, we also received the control Ad-EGFP adenovirus for free. In the future, I will not use any more siRNA or classical transfection methods on primary mouse hepatocytes, just adenoviruses from VB! I can highly recommend VectorBuilder's services for vector design and adenovirus production.
University of Würzburg, Germany
We have used VectorBuilder for multiple vectors including regular, lentiviral, Tet-inducible, and CRISPR/Cas9 vectors. Designing the vectors is super easy and we have had good luck with them. I would definitely recommend their products!
Texas A&M University, USA
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Dailing et al.
ACS Appl Mater Interfaces. PMID: 33124813 (2020).
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Matsuoka et al.
J Physiol. PMID: 33151574 (2021).
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