Firefly Luciferase IVT mRNA

Our HiExpress™ firefly luciferase mRNA was generated with or without modified nucleotide, N1-Methylpseudouridine (m1Ψ). The firefly luciferase mRNA has been sequence optimized and fully validated for high levels of expression both in vitro and in vivo.

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Click here to view the amino acid sequence translated from HiExpressTM Firefly Luciferease mRNA >>

Shipping and storage

Our IVT mRNA products are stored in a 1 mM sodium citrate buffer (pH 6.4) and can be stored at -80°C for up to 12 months. The products are shipped on dry ice and should be avoided for freeze-thaw cycles.

Experimental Validation

Luciferase activity in HEK293T cells and C57BL/6 mice being transfected with Firefly Luciferase mRNAs.

Firefly Luciferase IVT mRNA expression in vitro and in vivo

(A) Expression of HiExpress™ Firefly Luciferase mRNA and other luciferase mRNA in HEK293T cells. Cells grown on a 12-well plate were transfected with 0.5 ug of mRNA per well and luciferase activity was measured at 6 h, 24 h, 48 h, and 72 h post transfection. (B) Luciferase activity measured in adult C57BL/6 mice injected intramuscularly with 30 ug of LNP encapsulated mRNA at 6 h, 24 h, and 48 h post injection. FLuc WT indicates wild-type firefly luciferase. FLuc WT (co) indicates codon-optimized wild-type firefly luciferase. FLuc2 indicates Luc2 firefly luciferase.

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