EGFP LNP-mRNA

VectorBuilder's EGFP LNP-mRNA has been sequence optimized, fully QC'ed, and functionally validated. It can be used as a control for both in vivo and in vitro applications and can be ordered with or without modified nucleotide.

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Click here to view the amino acid sequence translated from EGFP mRNA >>

Shipping and storage

Our LNP-mRNA products are stored in Tris buffer (pH 7.4) and can be stored at -80°C for up to 6 months. The products are shipped on dry ice and should be avoided for freeze-thaw cycles.

Experimental Validation

Highly efficient mRNA delivery using LNP

Figure 1. Comparing the cellular expression levels of EGFP mRNA, LNP-encapsulated mRNA demonstrated higher expression compared to EGFP mRNA delivered by a commercial transfection reagent. 1 ug of EGFP mRNA was transfected into cells followed by (A) Flow cytometry analysis of EGFP expression in Jurkat and HEK293T cells and (B) Fluorescent imaging of HEK293T cells 24 hours post-transfection. Nucleotides of EGFP mRNA are both unmodified. Median Fluorescence Intensity (MFI).

Particle size and Zeta potential were measured for LNP-EGFP IVT mRNA.

Figure 2. Representative quality control (QC) results of EGFP LNP-mRNA. (A) Particle size was determined by dynamic light scattering (DLS) which measures the intensity differences of fluctuated light due to motion of particles. The polydispersity index (PDI) reflects the heterogeneity of a sample on particle size. (B) Zeta potential reflects the stability of LNP. The Zeta potential of the sample was between -1.872 mV and +1.872 mV.