HiExpress™ Cre IVT mRNA

CRE recombinase is a ~38 kD Type I topoisomerase encoded by bacteriophage P1 which recognizes two recombinase binding elements (RBE) within LoxP sites to facilitate site-specific recombination. Our HiExpress™ Cre IVT mRNA has been codon optimized to ensure high levels of CRE recombinase expression both in vitro and in vivo.

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Click here to view the amino acid sequence translated from HiExpress^TM Cre IVT mRNA >>

Shipping and storage

Our IVT mRNA products are stored in a 1 mM sodium citrate buffer (pH 6.4) and can be stored at -80°C for up to 12 months. RNA products are shipped on dry ice and freeze-thaw cycles should be avoided.

Experimental Validation

HiExpress Cre mRNA and LNP-mRNA expression

Figure 1. HiExpress^TM Cre mRNA and LNP-mRNA expression. (A) Expression levels of WT, Competitor T (m1Psi modified), and HiExpress^TM Cre mRNA (m1Psi modified) in HEK293T cells were measured 24 hours post-transfection by Western Blot. (B) Cre-dependent tdTomato expressions in Ai9 mice. Adult male Ai9 mice harboring loxP-flanked STOP upstream of CAG>tdTomato were intravenously injected with either 0.1 mg/kg or 0.4 mg/kg of Cre or HiExpress^TM Cre LNP-mRNA to evaluate tdTomato expression. (C) Representative images show liver tdTomato expression 48 hours post-injection.