EGFP IVT mRNA
VectorBuilder offers a variety of EGFP IVT mRNA, both unmodified and modified, for cell culture and zebrafish in vivo applications. Fully validated by our experts, these products ensure high EGFP expression.
Ordering Information Price Match
EGFP IVT mRNA
Zebrafish EGFP IVT mRNA
Click here to view the amino acid sequence translated from EGFP mRNA >>
Shipping and storage
Our IVT mRNA products are stored in a 1 mM sodium citrate buffer (pH 6.4) and can be stored at -80°C for up to 12 months. RNA products are shipped on dry ice and freeze-thaw cycles should be avoided.
Click here to view the amino acid sequence translated from EGFP mRNA >>
Shipping and storage
Our IVT mRNA products are stored in a 1 mM sodium citrate buffer (pH 6.4) and can be stored at -80°C for up to 12 months. RNA products are shipped on dry ice and freeze-thaw cycles should be avoided.
Experimental Validation
- EGFP mRNA
- Zebrafish EGFP mRNA
Figure 1. HeLa cells grown on a 12-well plate were transfected with 1 ug of mRNA per well. (A) EGFP expression at 24 h, 48 h, and 72 h post-transfection quantified by flow cytometry. Mean fluorescence intensities (MFI) are represented by colored bars and percentages of EGFP positive cells are represented by circles, squares, and triangles. Error bars indicate standard deviations (SD). (B) Representative images of HeLa cells transfected with EGFP mRNA at 48 h post-transfection.
Figure 2. Zebrafish EGFP IVT mRNA expression in vitro and in vivo. (A) 293T cells grown on a 12-well plate were transfected with 1 ug of zebrafish EGFP mRNA per well. Representative images at 72 h post-transfection indicate that 110 nt polyA tail increases EGFP expression in vitro. (B) One-cell stage zebrafish embryos were screened for EGFP expression at 6 h post-microinjection of 250 pg of zebrafish EGFP IVT mRNA. Fluorescent images indicate that optimized Kozak sequence (version 2) increased EGFP expression in vivo.
Resources
Documents
Brochures & Flyers
Certificate of Analysis (COA)
Material Safety Data Sheet (MSDS)
