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VectorBuilder's EGFP IVT mRNA can be ordered with or without the modified nucleotide, N1-Methylpseudouridine (m1Ψ) and has been fully validated for high levels of EGFP expression in cell culture.

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Click here to view the amino acid sequence translated from EGFP mRNA >>

Shipping and storage

Our IVT mRNA products are stored in a 1 mM sodium citrate buffer (pH 6.4) and can be stored at -80°C for up to 12 months. The products are shipped on dry ice and should be avoided for freeze-thaw cycles.

Experimental Validation

Flow cytometry analysis & fluorescent images for HeLa cells being transfected with EGFP expression IVT mRNA.

Figure 1. HeLa cells grown on a 12-well plate were transfected with 1 ug of mRNA per well. (A) EGFP expression at 24 h, 48 h, and 72 h post-transfection quantified by flow cytometry. Mean fluorescence intensities (MFI) are represented by colored bars and percentages of EGFP positive cells are represented by circles, squares, and triangles. Error bars indicate standard deviations (SD). (B) Representative images of HeLa cells transfected with EGFP mRNA at 48 h post-transfection.

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Certificate of Analysis (COA)
Material Safety Data Sheet (MSDS)