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HiExpress™ Firefly Luciferase IVT mRNA

VectorBuilder's HiExpress™ Firefly Luciferase IVT mRNA can be ordered with or without the modified nucleotide, N1-Methylpseudouridine (m1Ψ) and has been sequence optimized and fully validated for high levels of expression both in vitro and in vivo.

Ordering Information Price Match

Click here to view the amino acid sequence translated from HiExpressTM Firefly Luciferease mRNA >>

Shipping and storage

Our IVT mRNA products are stored in a 1 mM sodium citrate buffer (pH 6.4) and can be stored at -80°C for up to 12 months. RNA products are shipped on dry ice and freeze-thaw cycles should be avoided.

Experimental Validation

Luciferase activity in HEK293T cells and C57BL/6 mice being transfected with Firefly Luciferase mRNAs.

Figure 1. (A) Expression of HiExpress™ Firefly Luciferase mRNA and other luciferase mRNA in HEK293T cells. Cells grown on a 12-well plate were transfected with 0.5 ug of mRNA per well and luciferase activity was measured at 6 h, 24 h, 48 h, and 72 h post transfection. (B) Luciferase activity measured in adult C57BL/6 mice injected intramuscularly with 30 ug of LNP encapsulated mRNA at 6 h, 24 h, and 48 h post injection. FLuc WT indicates wild-type firefly luciferase. FLuc WT (co) indicates codon-optimized wild-type firefly luciferase. FLuc2 indicates Luc2 firefly luciferase.

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