HiExpress™ Sleeping Beauty System
The Sleeping Beauty system allows for genomic integration with non-viral delivery using co-introduction of transposon DNA and transposase. VectorBuilder’s HiExpress™ Sleeping Beauty Transposase IVT mRNA encodes a sequence-optimized transposase for high activity in mammalian cells, enabling robust cut-and-paste transposition. To further enhance efficiency, VectorBuilder has developed closed-ended linear DNA (celDNA) as an improved donor format. Alternatively, Sleeping Beauty transposon plasmids can be designed in our Vector Design Studio.
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Shipping and storage
Our IVT mRNA products are stored in 1 mM sodium citrate buffer (pH 6.4), while celDNA products are stored in nuclease-free water. Both can be stored at -80°C for up to 12 months. Products are shipped on dry ice, and freeze-thaw cycles should be avoided.
Experimental Validation
Figure 1. HiExpress™ Sleeping Beauty (SB) transposase IVT mRNA enables efficient transposition with celDNA donors. SB transposon celDNA (CMV>EGFP-T2A-Puro) was prepared by cell-free DNA amplification and transfected into HEK293T cells, with or without HiExpress™ SB transposase IVT mRNA. Cells were then split and subjected to puromycin selection 72 h post-transfection. EGFP expression was imaged using fluorescence microscopy.
Figure 2. Validation of HiExpress™ Sleeping Beauty Transposase IVT mRNA. Sleeping Beauty plasmids, pSB-CMV>EGFP, were transfected into HEK293T cells with or without HiExpress™ Sleeping Beauty Transposase IVT mRNA. The expression of EGFP was observed under microscope (A), and the mean fluorescence intensity was quantified using flow cytometry (B) at 24, 48, and 72 hours post-transfection.
Resources
Documents
Brochures & Flyers
Certificate of Analysis (COA)
Material Safety Data Sheet (MSDS)
