Zebrafish EGFP IVT mRNA

Name: Zebrafish EGFP IVT mRNA
Brand: VectorBuilder

VectorBuilder’s zebrafish EGFP mRNA was generated with optimized polyA tail and Kozak sequence. The high expression of EGFP has been validated both in vitro and in vivo.

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Click here to view the amino acid sequence translated from EGFP mRNA >>

Shipping and storage

Our IVT mRNA products are stored in a 1 mM sodium citrate buffer (pH 6.4) and can be stored at -80°C for up to 12 months. RNA products are shipped on dry ice and freeze-thaw cycles should be avoided.

Experimental Validation

Zebrafish EGFP IVT mRNA expression in vitro and in vivo

(A) 293T cells grown on a 12-well plate were transfected with 1 ug of zebrafish EGFP mRNA per well. Representative images at 72 h post-transfection indicate that 110 nt polyA tail increases EGFP expression in vitro. (B) One-cell stage zebrafish embryos were screened for EGFP expression at 6 h post-microinjection of 250 pg of zebrafish EGFP IVT mRNA. Fluorescent images indicate that optimized Kozak sequence (version 2) increased EGFP expression in vivo.