Testimonials

VectorBuilder is proud to share our client's testimonials:

  • VectorBuilder has been instrumental in supporting my research by providing timely and reliable vector cloning and virus packaging services. Joaquin is always responsive to my requests and vector design needs, efficiently addressing them with care and attention to detail. His dedication to the success of my projects has contributed to several publications. I highly recommend their services.

    Dr. Bogang Wu

    Harvard Medical School, USA

  • VectorBuilder is truly exceptional, offering best-in-class services for viral vector generation. Their contributions are so integral to our research that I consider them an invaluable extension of our lab.

    Dr. Garron Dodd

    The University of Melbourne, Australia

  • VectorBuilder’s plasmid and virus preparation services are reliable, competitively priced, and exceptional at handling challenging constructs. Their customer service is top-notch, making them an essential part of our workflow.

    Dr. Leesa Sampson

    Vanderbilt Genome Editing Resource, USA

  • VectorBuilder was fantastically helpful for our AAV experiments - wonderful for talking through our goals and proposed order, and very responsive in keeping us up-to-date on our order's progress. The AAV worked very well, and working with VectorBuilder (and especially Maria Pascual) was a very positive experience!

    Constantine Tzouanas

    Massachusetts Institute of Technology, USA

  • VectorBuilder's AAV serotype panel helped us to choose the best expressing serotype for our brain area and animal model of interest. By piloting injections using a selection of serotypes provided in the panel, we obtained invaluable pilot data before ordering a large scale virus production. This allowed us to save a considerable amount of time and resources. The technical support was great, they answer our questions promptly provided great advice. VectorBuilder technical support team further help us designing and cloning our AAV viral vector. Our preliminary data allowed to map retrogradely labelled neurons in the amygdala using light-sheet microscopy. We would not hesitate to use VectorBuilder for our next projects.

    Dr. Cristina Martinez Gonzalez

    University of Edinburgh, United Kingdom

  • In our recent publication titled “Dietary ω3 Fatty Acids Inhibit Tumorigenesis in a MYCN-Driven Neuroblastoma Mouse Model” (Cancers 2024, 17, 362), we utilized the MYCN ORF expression vector custom-built by VectorBuilder, which played a vital role in driving the success of our study.

    Our lab frequently orders from VectorBuilder, and their service has always been outstanding. The team is highly responsive and collaborative and consistently delivers high-quality constructs and glycerol stocks with quick turnaround. They work hand-in-hand with us throughout the design and ordering process, ensuring precision and performance.

    The MYCN ORF vector was central to our project on MYCN-driven neuroblastoma, and it performed robustly in both in vitro and in vivo models. VectorBuilder’s products and support remain valuable assets in our molecular biology research.

    I highly recommend VectorBuilder to other scientists looking for dependable, custom-built vector solutions.

    Dr. Vishwaben Mukeshbhai Patel

    University of Texas at Austin, USA

  • The products were of high quality and we were able to use these to knockdown genes in vivo, and to understand roles of orphan/understudied proteins in vivo. The team was very responsive and professional.

    Dr. Haopeng Xiao

    Stanford University, USA

  • I would like to share with you all my experience with VectorBuilder. I do a lot of vector designs for different applications. VectorBuilder website is easy to use and offers efficient and fast vector design tools. In addition, VectorBuilder team is friendly and is open to accommodate special custom and bespoke requirements for vector modifications that are not readily available on the website. Finally, the price is more than reasonable, and I would recommend checking VectorBuilder website for cloning projects.

    Dr. Yaron Galanty

    Wellcome Trust/CRUK Gurdon Institute, University of Cambridge, UK

  • Since I started using VectorBuilder I've found their service great at every step, from the initial design tool for custom vectors all the way through the ordering process to receiving the vectors. Everybody I've contact with has been helpful and approachable, and I look forward to working with VectorBuilder again in the future!

    Dr. Chris Carnie

    University of Cambridge, UK

  • The scientific standards of VectorBuilder’s services are unquestionably high. The web interface to design vectors is intuitive and in fact so easy to use that it can even serve for educational purposes. VectorBuilder has pretty much covered everything one can think about when wanting to design an expression vector, it’s great to have all the versatility on one page. Turnaround time is fast and owing to their professionalism and customer friendly attitude I can recommend VectorBuilder’s services without reservation.

    Dr. Johnny Kim

    Max Planck Institute for Heart and Lung Research, Germany

  • VectorBuilder is the best platform for designing and synthesizing constructs. The online vector design tool is so convenient and fun to use. Ms. Joyce Zhang is always very professional in replying my questions and does it so timely. One time, something did not work out, the refund process was smooth and all the staff were very nice and professional. The constructs I asked for had two fluorescent proteins with two promotors, one has NLS and the other does not. I did transfection and it worked out very well. Joyce helped me all through this process, making my life very easy. Basically, I had many pleasant experiences with Vectorbuilder, and I strongly recommend using it. I had tried other companies, they turned out to be either slow or lacked strong technical support or are expensive. Vectorbuilder is not the cheapest, but considering the quality of its service, it is worth the money.

    Dr. Shanshan Zhu

    Johns Hopkins University, USA

  • We’ve been using VectorBuilder’s services for plasmid construction as well as AAV and lentiviral vector production for several years. Their high-quality products and reliable technical support have greatly accelerated our research and made working with them an excellent experience.

    Dr. Farnaz Shamsi

    New York University, USA

  • VectorBuilder is efficient and easy to use for the outsourcing of plasmid generations. We used the PiggBac system for transgenic modifications and we have had great success.

    Luis Cruz

    University of California San Diego, USA

  • It has been a great experience working with VB. I was one of the first to test their empty capsid (AAV VLP) service. For my batch capsids were nearly completely empty (>97% by AUC and TEM) and were thus a huge help for assay development as a negative control. I also want to highlight that my contact Matthew Wheeler was always very helpful and transparent for any queries I had and that the team is very agile. A big plus is also the very intuitive website. Overall, a big thumbs up for VectorBuilder from my side.

    Dr. Andrei Hutanu

    Roche, Switzerland

  • I have been using ready-to-use lentiviruses from Vectorbuilder and they are working great. I used them against iPSC-derived neurons, and the transduction and expression of transgenes are remarkable. The vector backbones provide multiple options to tag your GOIs with fluorescent proteins or implement antibiotic selection markers. Their vector designing tools are helpful. Customer service is reliable. Good luck!

    Dr. Utpal Das

    University of California San Diego, USA

  • We requested a custom AAV (4-YF) from VectorBuilder. The ordering process was easy and the customer support team were really helpful. Our virus had the expected titer and worked extremely well for our chosen application.

    Dr. Jessica Rodgers

    The University of Manchester, UK

  • VectorBuilder is an amazing way to save time normally dedicated to molecular biology. The website is easy to use, well-conceived and allows quick design in most cases. For more unusual requests, the team is energetic, reply promptly on every request, and always finds a way to help. Low prices and short timelines really make it possible to consider VectorBuilder for many cloning matters.

    Dr. Laura Israel

    Novartis, Switzerland

  • Since last year, our lab has started to use the VectorBuilder service, including vector design and synthesis. The vectors were produced quickly and with high quality. It was a very good experience.

    Weili Sun

    McGill University, Canada

  • VectorBuilder’s unparalleled service has been key to our success engineering cell-based drug delivery systems. Anil’s exceptional support ensured our complex designs were built to our specifications.

    Nick Bergo

    Buck Institute, USA

  • In our study, we utilized VectorBuilder’s miR-30-based tetracycline-inducible vectors to achieve tightly controlled and tunable gene knockdown. The vectors were delivered quickly, came fully sequence-verified, and demonstrated robust inducible expression with minimal leakiness. The miR-30 backbone allowed for efficient shRNA expression while maintaining physiological relevance, and the tetracycline-inducible system provided precise temporal control over gene silencing. VectorBuilder’s platform made vector design and customization seamless, and their products played a critical role in the success of our functional studies.

    Stephen Holland

    CHEO Research Institute, Canada

  • We've used VectorBuilder for three different lentiviral shRNA plasmids, and each one worked exactly as expected with reliable knockdown efficiency. The process was smooth from design to delivery, and their customer support was responsive and helpful throughout. Highly recommend them for high-quality custom vectors.

    Dr. Sonja Arneaud

    University of Texas Southwestern Medical Center, USA

  • Clone development from vectorbuilder.com is also sufficient and they have made several clones for our projects which were delivered timely and were correct. One clone was very complex and they were able to make it successfully with the guidance of their technical team.

    Dr. Lindsey Goodman

    Monte Rosa Therapeutics, USA

  • Vector Builder’s user-friendly vector design tool, quick turn-around times, and reliable products allow us to drive research forward with confidence. Aside from their products, the team at Vector Builder is readily available to answer questions, provide updates, and offer discounts where possible!

    Mary Zlotosch

    Monte Rosa Therapeutics, USA

  • VectorBuilder's virus preparation services are at the best price and very reliable, even with constructs that have inserts longer than the theoretical maximum of insertion into adenoviral vectors. Additionally, VectorBuilder's customer service has been extremely positive!

    Dr. Dylan Valencia

    Tufts Medical Center, USA

  • I’ve been using VectorBuilder’s virus packaging services for two years. Their viruses worked well and my projects are going on well. I’ve published two papers on SCI journals using VectorBuilder’s services. Thank you, VectorBuilder!

    Wei Zhang

    Zhejiang University, China

  • Two years ago, when another CRO was struggling to provide the requested clones that I ordered, I reached out to Vector Builder to try to make them for me. Very difficult clones to make due to many repeats of amino acids. They got the clones done for me with no issues and very quickly. I have been using Vector Builder since then. Along with this, working with Alejandro Izurieta (California Territory Manager) has been great. He is knowledgeable, and professional promptly answers my request and is courteous. I rely on him to get the service that I need from Vector Builder done. He is just wonderful!

    Irena Levin

    Takeda, USA

  • I am using services which include vector construction and virus packaging from VectorBuilder to generate engineered immune cells for cancer immunotherapy. The service is fast, considerate, and high-qualified. I highly recommend this company as a reliable service provider and thank the whole team for the commitment to meeting our needs.

    Dr. Jiao Wang

    Purdue University, USA

  • Our research challenge

    Our work involves primary brain cells, including neurons, human astrocytes, and endothelial cells. These cells are notoriously challenging to maintain and to transduce with viruses for gene overexpression or downregulation.

    How VectorBuilder helped to solve this challenge

    VectorBuilder provided a wide range of lentiviral constructs for gene overexpression and downregulation, which allowed us to specifically target genes in primary brain cells. The viral vectors and particles are highly efficient, easy to handle, and facilitated our research significantly.

    Our experience with VectorBuilder

    We are extremely satisfied with the service provided by VectorBuilder. All of the vectors and viruses have performed reliably, and customer support has been excellent—always responsive and helpful with troubleshooting. Having used VectorBuilder’s services for over five years, we are very pleased with the quality and consistency of your service and would greatly appreciate any incentives or discounts on future purchases.

    Dr. Tsvetkov lab

    The University of Texas Health Science Center at Houston, USA

  • VectorBuilder is my favorite company! The service is reliable, efficient and excellent. The website user interface is intuitive. The number of choices for different vectors and downstream services is exhaustive, and the prices are reasonable. Furthermore, the team is receptive to feedback and queries. Compared to the old days of cloning, VectorBuilder now saves us enormous time and money, and frees us up to focus on the experimental questions rather than getting bogged down with making constructs.

    Dr. Timothy Cripe

    Nationwide Children’s Hospital, USA

  • We are testing different vectors for T cell therapy, and VectorBuilder provides a one stop solution for design, construct and manufacturing numerus lentiviral vectors for us. One of our vector was GMP manufactured by VectorBuilder and move to FDA approved clinical trial. We had great experience working with VectorBuilder, from communication to product QC, high level of service.

    Xueqing Xia

    UT MD Anderson Cancer Center, USA

  • I have been a frequent vectorbuilder customer for the last three years. Their vectors and lentivirus preps have always been reliable and high titer, and I have had a lot of experimental success using their products. Above all else, their customer service is excellent. They are very responsive and have assisted me innumerable times with vector design and other questions, offered me discounts, etc. Vectorbuilder has been instrumental to my experimental success in the lab.

    Dr. Charles Danan

    Children's Hospital of Philadelphia, USA

  • VectorBuilder has greatly assisted our research, facilitating hypothesis testing through intramuscular injections of recombinant adeno-associated viral serotype 6 (rAAV6):Slirp vectors. The support from their team ensured that the vector met our needs, and their production methods delivered reliable results. We appreciate their valuable contribution to our research.

    Dr. Tang Cam Phung Pham

    University of Copenhagen, Denmark

  • Vectorbuilder is reliable, fast, and the price is super reasonable. I want to thank Vectorbuilder for providing such excellent service!

    Dr. Weiwen Zhang

    Emory University, USA

  • We have used VectorBuilder for multiple vectors including regular, lentiviral, Tet-inducible, and CRISPR/Cas9 vectors. Designing the vectors is super easy and we have had good luck with them. I would definitely recommend their products!

    Dr. Steven Wall

    Texas A&M University, USA

  • We were working on nascent DNA processing at stalled replication forks in BRCA-deficient cells and used a lentiviral construct and CRISPR/Cas9-mediated genome editing from VectorBuilder to create our knockout cell lines. The VectorBuilder support team have great technical expertise, are very supportive and fantastic to work with. I highly recommend VectorBuilder's services to accelerate research projects.

    Dr. George-Lucian Moldovan

    The Pennsylvania State University, USA

  • I have been using Vector Builder to purchase vectors designed to express shRNA. I have found the technical support very helpful and friendly. They will make suggestions that will improve the vectors I design. Customer support is also quite fast in their responses to my questions and have also been quite accomodating with some of my requests. They also provide very helpful diagrams of their vectors that make it easy for anyone to visualize. I highly recommend Vector Builder.

    Dr. Victor Ruiz-Velasco

    Penn State College of Medicine

  • Before using VectorBuilder, producing lentiviral overexpression vectors for our in vitro models was a recurring and time-consuming enterprise. We faced frequent challenges in every experimental step, which made it difficult to plan the actual experiments. Setting up the process with VectorBuilder was very efficient, thanks to their great expertise and support. Once we started using VectorBuilder's services, we noticed a substantial increase in productivity as well as a more uniform and predictable transduction pattern of our target cells.

    We went on to use VectorBuilder for a large-scale CRISPR screen with a custom library spanning over 1,600 genes. With the provided virus, we were able to easily maintain cell representation and we derived a number of interesting hit genes, which we are excited to follow up on. A big thank you to the entire VectorBuilder Team!

    Dr. Dominic Schmid

    University of Basel

  • We ordered adenovirus from Vectorbuilder company to overexpress our interest genes. The service is fast, considerate, and high-qualified. Besides, the adenovirus is very effective to overexpress our target gene.

    Dr. Wanbao Yang

    Texas A&M University, USA

  • I am very satisfied with the constructions (double mutants) made by Vectorbuilder. The service is very serious and rigorous. They started by verifying our plasmid by doing digestions. As this was not enough, they even sequenced part of the plasmid, which then ensured the success of the constructs. Constant email exchanges for verification steps were ensured with almost instantaneous responsiveness! Quite impressive! Once the sequence was clarified, production was not long, and we were able to do our experiments under excellent conditions. The results were as good as we expected. Bravo again and thank you for the efficiency, the rigor, and the seriousness.

    Sabine Levi

    French National Centre for Scientific Research (CNRS), France

  • Our lab has been using VectorBuilder to aid in the design and synthesis of plasmids for the last 3-4 years. They have always been extremely helpful with design requests. I would highly recommend VectorBuilder's services to anyone wanting to accelerate their research progress.

    Dr. Andrew Cox

    Peter MacCallum Cancer Centre, Australia

  • VectorBuilder’s service makes designing vectors incredibly simple and there’s no software required, it can all be done through their easy to use website. The lentivirus production service is a massive bonus as it’s so quick and reasonably priced. There’s really no reason to make our own lentivirus anymore. Thanks for a fantastic service.

    Dr. Timothy London

    TC Biopharm, UK

  • We routinely turn to VectorBuilder for the design and production of vectors used to overexpress or silence genes in our translational research on multiple myeloma. Their team has been consistently helpful in addressing technical hurdles related to gene delivery and expression in our models.

    Working with VectorBuilder has always been a smooth experience — communication is clear, timelines are reliable, and the quality of the vectors has been key to the success of several experiments. They’ve become a trusted partner in our work.

    Dr. Debora Soncini

    University of Genoa, Italy

  • We utilized two lentiviral vectors, lenti-CYP and lenti-Ctr, from VectorBuilder Inc. in our study on Lgr5+ intestinal stem cells. The lenti-CYP vector, encoding the hCYP27B1 gene, enabled de novo synthesis of locally high 1,25(OH)2D concentrations, proving crucial for our investigation. The high-quality design, including promoter specificity and integrated resistance genes, ensured reliable gene expression and successful transduction. The control vector, lenti-Ctr, further allowed precise experimental comparisons. VectorBuilder's products significantly contributed to the success of our research, and I highly recommend their custom vector services for advanced molecular biology applications.

    Dr. Shaikh Nisar Ali

    Long Island University, USA

  • Using VectorBuilder has transformed the way I do my research. VectorBuilder’s easy-to-use web interface allows me to custom build my own plasmids, freeing up time that I would have wasted cloning and instead letting me focus on the research that really matters. They offer great prices with a fast turnaround time. Their friendly and well-informed technical support team are on hand every step of the way, should you need any help. I would strongly recommend VectorBuilder to anyone wanting to accelerate the progress of their research.

    Dr. David M Brown

    University of Nottingham, UK

  • We ordered vectors from Vectorbuilder already many times. We are happy with the service. The vectors work well and service is fast. I would highly recommend Vectorbuilder. We have very good experience with them.

    Dr. Rene Opavsky

    University of Florida, USA

  • VectorBuilder is an intuitive tool for developing vectors at affordable prices. I was in need of a CRISPR lentiviral system with a Puro selection cassette due to working with delicate cells that cannot handle FACS sorting pressures. With their online interface, it was easy to construct the vector I wanted and received the vector in a timely manner! It was simple to produce virus and test it in our cell culture and generated clonal cells with good gene knockdown. I definitely will be ordering more vectors from VectorBuilder to finish up my thesis!

    Dr. Christophe Langouet-Astrie

    University of Colorado, USA

  • We recently purchased a number of customized plasmids on VectorBuilder. They consisted of expression vectors of two or more ORFs. Our experience was satisfactory, efficient and cost-effective.

    The designing stage was facilitated by the VectorBuilder design tool online, in which many of the most commonly used sequences, like fluorescent proteins, selection drug resistance genes, and fusion or cleavage linkers were readily offered to be picked from the ORF lists. Regarding our specific sequences, once pasted into the corresponding window of the design tool, they were immediately matched with the International Nucleotide Sequence Database Collaboration (DDBJ/EMBL/GenBank) accession numbers and compared to the databases. Where there were discrepancies, we were asked which transcripts/cDNAs we preferred to use.

    After completing the order, in a matter of three to four weeks, we had in our hands 8 of the 10 plasmids, as offered. For the rest of the plasmids we provided the template for a specific sequence. Before proceeding with the cloning, they sequenced it, detected a mutation and corrected it at a low extra cost upon our approval. A week later these corrected plasmids were delivered as well. During the whole process, VectorBuilder’s European team kept a personalized email dialogue with us, enabling us to follow-up and make decisions or accept suggestions about the plasmids.

    We have satisfactorily tested every plasmid now. We think that our decision to work with VectorBuilder has boosted our research by saving us time, resources and enabling us to concentrate more on our experiments.

    Dr. Leonel Muñoz Sagredo

    The Karlsruhe institute of Technology, Germany

  • VectorBuilder's service is simply amazing. Even if you only have an idea of what you want to do, the VectorBuilder experts will help you make it happen. The service is straightforward and really accommodating. This includes fast delivery and quick responses to queries. I recommend the service to all my colleagues and will definitely use them again for future projects.

    Dr. Stephan Kuenzel

    TU Dresden, Germany

  • Working with VectorBuilder has been a fantastic experience for our lab's vector design and AAV packaging needs. Their expertise and efficiency in handling our project were invaluable, especially given our complex requirements. The team was incredibly responsive and thorough, and Joseph Perez, in particular, was outstanding in guiding us through every step and addressing all of our questions. Thanks to their support, we received high-quality vectors in a timely manner, which has allowed us to move forward smoothly with our research.

    Dr. Yutong Shang

    Case Western Reserve University, USA

  • We ordered adenovirus from VectorBuilder to overexpress our protein of interest in primary mouse hepatocytes. The ordering process, including vector design, was very fast and easy. We received the plasmid and adenovirus within the time frame that was stated during the ordering process and the transduction efficiency of the adenovirus was very high, enabling us to use a low MOI. As a bonus, we also received the control Ad-EGFP adenovirus for free. In the future, I will not use any more siRNA or classical transfection methods on primary mouse hepatocytes, just adenoviruses from VB! I can highly recommend VectorBuilder's services for vector design and adenovirus production.

    Alexander Mayer

    University of Würzburg, Germany

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