Testimonials

VectorBuilder is proud to share our client's testimonials:

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  All AAV AAV Serotype Testing Panel Adenovirus CRISPR/Cas9 Genome Editing Solutions Customer Service Lentivirus Library Construction Online Vector Design Plasmid DNA Preparation shRNA Gene Knockdown Solutions Technical Support Vector Cloning Vector Design Support Virus Packaging

  All AAV AAV Serotype Testing Panel Adenovirus CRISPR/Cas9 Genome Editing Solutions Customer Service Lentivirus Library Construction Online Vector Design Plasmid DNA Preparation shRNA Gene Knockdown Solutions Technical Support Vector Cloning Vector Design Support Virus Packaging

• I would like to share with you my positive experience purchasing a lentiviral vector from VectorBuilder.
   
  First, I am an immunologist and have little experience with cloning. In my recent project, we needed to overexpress a gene in melanoma cells. The transfection efficiency using plasmids in this cell line was extremely low, less than 10%. Then, we tried to use antibiotics to select transfected cells and establish stably expressing cell lines. Unfortunately, for unknown reasons, the ‘stable transfectants’ lost the transfected genes! I was frustrated at this point, until I met VectorBuilder at the EACR conference in Amsterdam. Using the user-friendly website from VectorBuilder, I designed for the first time in my life my own lentiviral vector and got the product in less than one month. My group leader, who has a lot of cloning experience, could not believe that it worked so quickly. Of course, most important are the experimental results. To access the transduction efficiency, I included an EGFP tag in the vector. Now, I can transduce the melanoma cells with nearly 100% positivity. In the following assays, the genes transduced into the tumor cells resulted in a 6-fold induction in tumor immunogenicity.
  Taken together, I strongly recommend VectorBuilder to researchers, especially those who are not so experienced with cloning, and am convinced that the service and product quality from VectorBuilder can further help me to proceed with my own projects more effectively.

  Fang Zhao

  UK-Essen, Germany

• I have been consistently using VectorBuilder over the last couple of years and have been extremely satisfied with the vectors they've delivered. These include standard over-expression vectors, and Cas9 vectors on the PiggyBac backbone. All of the vectors I have purchased so far have passed my QC and provided reproducible functional data.

  Daksh Thaper

  Vancouver Prostate Centre, Canada

• VectorBuilder is the best platform for designing and synthesizing constructs. The online vector design tool is so convenient and fun to use. Ms. Joyce Zhang is always very professional in replying my questions and does it so timely. One time, something did not work out, the refund process was smooth and all the staff were very nice and professional. The constructs I asked for had two fluorescent proteins with two promotors, one has NLS and the other does not. I did transfection and it worked out very well. Joyce helped me all through this process, making my life very easy. Basically, I had many pleasant experiences with Vectorbuilder, and I strongly recommend using it. I had tried other companies, they turned out to be either slow or lacked strong technical support or are expensive. Vectorbuilder is not the cheapest, but considering the quality of its service, it is worth the money.

  Shanshan Zhu

  Johns Hopkins University, USA

• Amazing experience with VectorBuilder! I ordered plasmids at the beginning of December, and despite the holiday season they arrived exactly as scheduled. Furthermore, customer support reached out to ask what would be my desired delivery date, considering potential holiday conflicts at my institute. Overall, I am very satisfied, would highly recommend VectorBuilder (which I already do) and will buy from them again. All the best from my side!

  Tamara Tomin

  Medical University of Graz, Austria

• VectorBuilder is efficient and easy to use for the outsourcing of plasmid generations. We used the PiggBac system for transgenic modifications and we have had great success.

  Luis Cruz

  University of California San Diego, USA

• Using VectorBuilder has transformed the way I do my research. VectorBuilder’s easy-to-use web interface allows me to custom build my own plasmids, freeing up time that I would have wasted cloning and instead letting me focus on the research that really matters. They offer great prices with a fast turnaround time. Their friendly and well-informed technical support team are on hand every step of the way, should you need any help. I would strongly recommend VectorBuilder to anyone wanting to accelerate the progress of their research.

  David M Brown

  University of Nottingham, UK

• We recently purchased a number of customized plasmids on VectorBuilder. They consisted of expression vectors of two or more ORFs. Our experience was satisfactory, efficient and cost-effective.

  The designing stage was facilitated by the VectorBuilder design tool online, in which many of the most commonly used sequences, like fluorescent proteins, selection drug resistance genes, and fusion or cleavage linkers were readily offered to be picked from the ORF lists. Regarding our specific sequences, once pasted into the corresponding window of the design tool, they were immediately matched with the International Nucleotide Sequence Database Collaboration (DDBJ/EMBL/GenBank) accession numbers and compared to the databases. Where there were discrepancies, we were asked which transcripts/cDNAs we preferred to use.

  After completing the order, in a matter of three to four weeks, we had in our hands 8 of the 10 plasmids, as offered. For the rest of the plasmids we provided the template for a specific sequence. Before proceeding with the cloning, they sequenced it, detected a mutation and corrected it at a low extra cost upon our approval. A week later these corrected plasmids were delivered as well. During the whole process, VectorBuilder’s European team kept a personalized email dialogue with us, enabling us to follow-up and make decisions or accept suggestions about the plasmids.

  We have satisfactorily tested every plasmid now. We think that our decision to work with VectorBuilder has boosted our research by saving us time, resources and enabling us to concentrate more on our experiments.

  Leonel Munoz

  The Karlsruhe institute of Technology, Germany

• I have been using VectorBuilder's cloning service for several years and am extremely happy with their products and services. Their online design software is very easy to use and provides the most cutting edge solutions for today's most demanding molecular cloning needs. Their customer service is outstanding, with great technical competence and the delivery of my vectors was on time. I have saved months of precious research time by using VectorBuilder. I would recommend VectorBuilder to anyone who wants the best vector design and molecular cloning services.

  Guohao Dai

  Northeastern University, USA

• VectorBuilder’s service makes designing vectors incredibly simple and there’s no software required, it can all be done through their easy to use website. The lentivirus production service is a massive bonus as it’s so quick and reasonably priced. There’s really no reason to make our own lentivirus anymore. Thanks for a fantastic service.

  Timothy London

  TC Biopharm, UK

• Our laboratory has had great success with both viral constructs and ready-to-use virus prepared by VectorBuilder. They provide excellent customer service at a reasonable price, and their website is extremely user-friendly. Very pleased we discovered this company.

  Marco Seandel

  Weill Cornell Medical College, USA

• We requested a custom AAV (4-YF) from VectorBuilder. The ordering process was easy and the customer support team were really helpful. Our virus had the expected titer and worked extremely well for our chosen application.

  Jessica Rodgers

  The University of Manchester, UK

• We were searching for a way to outsource the cloning of our vectors and we are very happy with the service. The versatility of the tools make it a great time saver and the vectors were delivered on-time. Lentiviruses packaged are also very reliable and have been very successful in transduction studies in primary cells. Great customer service with fast response times and the team at VectorBuilder are very knowledgeable.

  Kathy Ngo

  Aridis Pharmaceuticals, USA

• I have been successfully cloning for 3+ years, but the last time we tried generating KO cell lines with CRISPR/Cas9 vectors, we were not successful. This was upsetting, especially since I invested a lot of time in these efforts. Then, by chance, I attended one of VectorBuilder's seminars. Due to their reasonable prices and the fact I could focus my time on other work, we decided to give their lentiviral (dual gRNA) Cas9 plasmid a try. The turnaround time was reasonable, their customer service was excellent and they actually gave feedback about the status of my cloning projects. Their website is also easy to use. But most importantly: using their Cas9 dual gRNA vector, I was able to successfully generate KO cell lines! 

  I recommend VectorBuilder to CRISPRCas9 users without any hesitation. 

  Anna Salamero Boix

  Georg-Speyer-Haus Frankfurt, Germany

• We have ordered several vectors from VectorBuilder, including regular expression plasmids, targeting vectors and AAVs. The constructs were delivered on time and worked very well. They saved us a lot of time and money. Their design team pays attention to detail and made very useful suggestions. Their customer service is excellent as well. Overall, we highly recommend VectorBuilder.

  Martin Riccomagno

  University of California, Riverside, USA

• We applied CRISPR cas9 nickase in our study. VectorBuilder helped us design the gRNAs and construct plasmids with double gRNAs and double selection markers (EGFP/puromycin). The gRNA specificity is quite good, better than available online design tools. We got the knockout cell lines in the first run of electroporation with no off-target events. I would recommend VectorBuilder to CRISPR cas9 users.

  Jie Qin

  University Clinic Cologne, Germany

• I ordered a plasmid vector two years ago. The design on the web platform was easy. Contacts with the specialists to arrange further details were excellent: they were very supportive!

  Emanuela Signori

  Consiglio Nazionale delle Ricerche, Italy

• I’ve always been using VectorBuilder’s vector construction service. I’m generally satisfied with the quality of its service, and the problems I encountered were solved quickly.

  Huimin Chen

  The First Affiliated Hospital, Sun Yat-sen University, China

• We rely on VectorBuilder for high-quality molecular biology reagents. The constructs and particles are delivered to us in a timely manner. They have been more than willing to generate new plasmids and viruses if they do not work well. In summary, we have been very satisfied with VectorBuilder.

  Sathish Srinivasan

  Oklahoma Medical Research Foundation, USA

• VectorBuilder's online vector design tool is very efficient, quick and reliable. Special thanks to the French territory manager who is easy to communicate with and gave us very useful advice.

  Laure LECOIN

  Collège de France, France

• The services provided by VectorBuilder are excellent. I had a few vector constructs made by the VectorBuilder. The description of the constructs by their software is excellent. The technical team of the company seems to have a sound knowledge and the technical suggestion regarding vector construction given to me by them were excellent.

  Saeed-ul-Hassan Khan

  Quaild-i-Azame University, Pakistan

• I’ve used VectorBuilder’s lentivirus packaging service. The virus titer was as promised. Strong fluorescence was observed after transduction. The gene knockdown efficiency was also satisfactory.

  Yunhao Liang

  Southern Medical University, China

• VectorBuilder is an amazing way to save my time for the generation of my plasmid and provide excellent downstream services. You can get any custom designed vectors you want at a very good price. I am sure I will order the vectors I need in the near future.

  Pengchao Zhang

  Houston Methodist Research Institute, USA

• I purchased a lentivirus vector and received it in less than two weeks. I am so glad that I found VectorBuilder, so I can design my lentiviral vector by picking any promotor, and from a variety of antibiotic selection markers and fluorescent tags. Upon transducing several cell lines, my target genes were highly and stably expressed with expression persisting for at least after one month after undergoing selection.

  Xianghong Chen

  H. Lee Moffitt Cancer Center & Research Institute, USA
  

• VectorBuilder is an intuitive tool for developing vectors at affordable prices. I was in need of a CRISPR lentiviral system with a Puro selection cassette due to working with delicate cells that cannot handle FACS sorting pressures. With their online interface, it was easy to construct the vector I wanted and received the vector in a timely manner! It was simple to produce virus and test it in our cell culture and generated clonal cells with good gene knockdown. I definitely will be ordering more vectors from VectorBuilder to finish up my thesis!

  Christophe Langouet-Astrie

  University of Colorado, USA

• VectorBuilder has provided very satisfactory service in making various AAV vector constructs for us. The new sales representative is very proactive in offering help on explaining and introducing other VectorBuilder service to us. Expecting to get more support and service from VectorBuilder.

  Qingchun Tong

  University of Texas Medical School at Houston, USA

• Thumbs up! I chose VectorBuilder after comparing it with other companies, because it provides high-quality products and services at unbeatable prices.

  Lihua Xu

  The First Affiliated Hospital of Guangzhou Medical University, China

• I’ve used VectorBuilder’s vector construction and lentivirus packaging services. The turnaround time was short and everything worked well. Also, I received quick responses from the customer support team.

  Xijuan Chen

  Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, China

• VectorBuilder has helped with my research progress. Cloning hasn't been an issue in our lab until recently. Their construct worked well. They saved me a lot of time and provide great services!

  Jennifer Cho

  University of California, Riverside, USA

• I would like to share with you all my experience with VectorBuilder. I do a lot of vector designs for different applications. VectorBuilder website is easy to use and offers efficient and fast vector design tools. In addition, VectorBuilder team is friendly and is open to accommodate special custom and bespoke requirements for vector modifications that are not readily available on the website. Finally, the price is more than reasonable, and I would recommend checking VectorBuilder website for cloning projects.

  Yaron Galanty

  Wellcome Trust/CRUK Gurdon Institute, University of Cambridge, UK

• We ordered vectors from Vectorbuilder already many times. We are happy with the service. The vectors work well and service is fast. I would highly recommend Vectorbuilder. We have very good experience with them.

  Rene Opavsky

  University of Florida, USA

• I used the lentivirus packaging service from VectorBuilder, and the process is very easy. All I needed to do is to ship a small quantity of transfer plasmid, and VectorBuilder will do the rest. Then I received the high titer virus at the end.

  Xueqing Xia

  UT MD Anderson Cancer Center, USA

• The scientific standards of VectorBuilder’s services are unquestionably high. The web interface to design vectors is intuitive and in fact so easy to use that it can even serve for educational purposes. VectorBuilder has pretty much covered everything one can think about when wanting to design an expression vector, it’s great to have all the versatility on one page. Turnaround time is fast and owing to their professionalism and customer friendly attitude I can recommend VectorBuilder’s services without reservation.

  Johnny Kim

  Max Planck Institute for Heart and Lung Research, Germany

• We ordered adenovirus from VectorBuilder to overexpress our protein of interest in primary mouse hepatocytes. The ordering process, including vector design, was very fast and easy. We received the plasmid and adenovirus within the time frame that was stated during the ordering process and the transduction efficiency of the adenovirus was very high, enabling us to use a low MOI. As a bonus, we also received the control Ad-EGFP adenovirus for free. In the future, I will not use any more siRNA or classical transfection methods on primary mouse hepatocytes, just adenoviruses from VB! I can highly recommend VectorBuilder's services for vector design and adenovirus production.

  Alexander Mayer

  University of Würzburg, Germany

• We have used VectorBuilder for multiple vectors including regular, lentiviral, Tet-inducible, and CRISPR/Cas9 vectors. Designing the vectors is super easy and we have had good luck with them. I would definitely recommend their products!

  Steven Wall

  Texas A&M University, USA

• Highly satisfied with service and products!

  Aarthi Pugazenthi

  Baylor College of Medicine, USA

• Vector Builder was fast, efficient and easy to use. We received our viral vector promptly and AAV9 has performed exactly how'd we hoped. Thank you!

  Alexander Alimov

  University of Kentucky, USA

• Rapid delivery and quality virus.

  Julie Sebag

  University of Iowa, USA

• I have great appreciate for VectorBuilder 1) User friendly, 2) Easy to design, and 3) Quick response for our questions and kind help.

  Makoto Kobayashi

  MD Anderson Cancer Center, USA

• We recently purchased a number of plasmids, of varying formats, from VectorBuilder. We were very happy with the service. We found VectorBuilder to be professional, efficient, and offer excellent value for money.

  Ian Lobb

  Almac Discovery, UK

• VectorBuilder did vector construction and lentivirus packaging for my shRNA knockdown experiment. The transduction efficiency of the lentivirus was high. Their staff were very considerate, and related technical questions were addressed quickly. I’ve finished many experiments and I’m preparing the manuscript for submission.

  Mengyuan Fan

  School of Nature Conservation, Beijing Forestry University, China

• I love VectorBuilder. Their service is exemplary and the sales rep is so helpful.

  Heather Hambright

  UT Health San Antonio, USA

• Since I started using VectorBuilder I've found their service great at every step, from the initial design tool for custom vectors all the way through the ordering process to receiving the vectors. Everybody I've contact with has been helpful and approachable, and I look forward to working with VectorBuilder again in the future!

  Chris Carnie

  University of Cambridge, UK

• I have been using ready-to-use lentiviruses from Vectorbuilder and they are working great. I used them against iPSC-derived neurons, and the transduction and expression of transgenes are remarkable. The vector backbones provide multiple options to tag your GOIs with fluorescent proteins or implement antibiotic selection markers. Their vector designing tools are helpful. Customer service is reliable. Good luck!

  Utpal Das

  University of California San Diego, USA
  

• I am using services which include vector construction and virus packaging from VectorBuilder to generate engineered immune cells for cancer immunotherapy. The service is fast, considerate, and high-qualified. I highly recommend this company as a reliable service provider and thank the whole team for the commitment to meeting our needs.

  Jiao Wang

  Purdue University, USA

• I must say, having dealt with many vector suppliers up until now, VectorBuilder has been by far the best to communicate rapidly, listening to client requests and going above and beyond wet lab work. As a relatively new company - Their Future is bright.

  Alan Griffith

  MeiraGTx, UK

• Vectorbuilder offers a unique platform for easy design of customized DNA vectors and is one of our first choice providers for molecular biology services. Consistently fast turnover times, high quality of services and excellent customer liaison are the decisive points for us to rely on Vectorbuilder's expertise. I can highly recommend Vectorbuilder as a reliable service provider and thank the whole team for the commitment to meeting our needs.

  Eugen Werwein

  Evotec SE, Germany

• VectorBuilder has provided incredible value and customer service throughout the entire process from product selection, custom design assistance, order submission, status reports and ultimate delivery management. The custom products I purchased from them performed exactly as advertised and in fact surpassed my expectations. I would recommend VectorBuilder to anyone requiring this type of product.

  Frank Borriello

  Alloplex Biotherapeutics, USA

• Excellent service with quality vectors that work well.

  Gulab Zode

  UNTHSC, USA

• VectorBuilder provides fast and economic service for plasmids construction, which saves a lot of time for me. I mostly construct lentivirus plasmids with them and so far have a positive impression for their products. They respond to my requests quickly and honestly so the communication with VectorBuilder’s staffs is one of the best parts of the service.

  Eiji Yoshihara

  Lundquist Institute at Harbor-UCLA Medical Center, USA

• I have been using Vector Builder to purchase vectors designed to express shRNA. I have found the technical support very helpful and friendly. They will make suggestions that will improve the vectors I design. Customer support is also quite fast in their responses to my questions and have also been quite accomodating with some of my requests. They also provide very helpful diagrams of their vectors that make it easy for anyone to visualize. I highly recommend Vector Builder.

  Victor Ruiz-Velasco

  Penn State College of Medicine