Testimonials

VectorBuilder is proud to share our client's testimonials:

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  All AAV AAV Serotype Testing Panel Adenovirus CRISPR/Cas9 Genome Editing Solutions Customer Service Lentivirus Library Construction Online Vector Design Plasmid DNA Preparation shRNA Gene Knockdown Solutions Technical Support Vector Cloning Vector Design Support Virus Packaging

  All AAV AAV Serotype Testing Panel Adenovirus CRISPR/Cas9 Genome Editing Solutions Customer Service Lentivirus Library Construction Online Vector Design Plasmid DNA Preparation shRNA Gene Knockdown Solutions Technical Support Vector Cloning Vector Design Support Virus Packaging

• We rely on VectorBuilder for high-quality molecular biology reagents. The constructs and particles are delivered to us in a timely manner. They have been more than willing to generate new plasmids and viruses if they do not work well. In summary, we have been very satisfied with VectorBuilder.

  Sathish Srinivasan

  Oklahoma Medical Research Foundation, USA

• I purchased a lentivirus vector and received it in less than two weeks. I am so glad that I found VectorBuilder, so I can design my lentiviral vector by picking any promotor, and from a variety of antibiotic selection markers and fluorescent tags. Upon transducing several cell lines, my target genes were highly and stably expressed with expression persisting for at least after one month after undergoing selection.

  Xianghong Chen

  H. Lee Moffitt Cancer Center & Research Institute, USA
  

• I would like to share with you my positive experience purchasing a lentiviral vector from VectorBuilder.
   
  First, I am an immunologist and have little experience with cloning. In my recent project, we needed to overexpress a gene in melanoma cells. The transfection efficiency using plasmids in this cell line was extremely low, less than 10%. Then, we tried to use antibiotics to select transfected cells and establish stably expressing cell lines. Unfortunately, for unknown reasons, the ‘stable transfectants’ lost the transfected genes! I was frustrated at this point, until I met VectorBuilder at the EACR conference in Amsterdam. Using the user-friendly website from VectorBuilder, I designed for the first time in my life my own lentiviral vector and got the product in less than one month. My group leader, who has a lot of cloning experience, could not believe that it worked so quickly. Of course, most important are the experimental results. To access the transduction efficiency, I included an EGFP tag in the vector. Now, I can transduce the melanoma cells with nearly 100% positivity. In the following assays, the genes transduced into the tumor cells resulted in a 6-fold induction in tumor immunogenicity.
  Taken together, I strongly recommend VectorBuilder to researchers, especially those who are not so experienced with cloning, and am convinced that the service and product quality from VectorBuilder can further help me to proceed with my own projects more effectively.

  Fang Zhao

  UK-Essen, Germany

• I have been successfully cloning for 3+ years, but the last time we tried generating KO cell lines with CRISPR/Cas9 vectors, we were not successful. This was upsetting, especially since I invested a lot of time in these efforts. Then, by chance, I attended one of VectorBuilder's seminars. Due to their reasonable prices and the fact I could focus my time on other work, we decided to give their lentiviral (dual gRNA) Cas9 plasmid a try. The turnaround time was reasonable, their customer service was excellent and they actually gave feedback about the status of my cloning projects. Their website is also easy to use. But most importantly: using their Cas9 dual gRNA vector, I was able to successfully generate KO cell lines! 

  I recommend VectorBuilder to CRISPRCas9 users without any hesitation. 

  Anna Salamero Boix

  Georg-Speyer-Haus Frankfurt, Germany

• We applied CRISPR cas9 nickase in our study. VectorBuilder helped us design the gRNAs and construct plasmids with double gRNAs and double selection markers (EGFP/puromycin). The gRNA specificity is quite good, better than available online design tools. We got the knockout cell lines in the first run of electroporation with no off-target events. I would recommend VectorBuilder to CRISPR cas9 users.

  Jie Qin

  University Clinic Cologne, Germany

• I have been consistently using VectorBuilder over the last couple of years and have been extremely satisfied with the vectors they've delivered. These include standard over-expression vectors, and Cas9 vectors on the PiggyBac backbone. All of the vectors I have purchased so far have passed my QC and provided reproducible functional data.

  Daksh Thaper

  Vancouver Prostate Centre, Canada

• Our laboratory has had great success with both viral constructs and ready-to-use virus prepared by VectorBuilder. They provide excellent customer service at a reasonable price, and their website is extremely user-friendly. Very pleased we discovered this company.

  Marco Seandel

  Weill Cornell Medical College, USA

• VectorBuilder has provided incredible value and customer service throughout the entire process from product selection, custom design assistance, order submission, status reports and ultimate delivery management. The custom products I purchased from them performed exactly as advertised and in fact surpassed my expectations. I would recommend VectorBuilder to anyone requiring this type of product.

  Frank Borriello

  Alloplex Biotherapeutics, USA

• We began using VectorBuilder for adenoviral plasmids construction about a year ago. We normally handled all cloning projects ourselves, but when it comes to adenoviral vectors, because of their large size, sometimes the cloning is kind of tricky for inexperienced students. So we decided to give VectorBuilder a try. The plasmid arrived one month later and the virus we generated from it expressed the correct gene. We have ordered more than 10 adenoviral plasmids and they all work fine. VectorBuilder has saved us a lot of time.

  Lei Sun

  DUKE-NUS Graduate Medical School, Singapore

• I ordered a plasmid vector two years ago. The design on the web platform was easy. Contacts with the specialists to arrange further details were excellent: they were very supportive!

  Emanuela Signori

  Consiglio Nazionale delle Ricerche, Italy

• I’ve always been using VectorBuilder’s vector construction service. I’m generally satisfied with the quality of its service, and the problems I encountered were solved quickly.

  Huimin Chen

  The First Affiliated Hospital, Sun Yat-sen University, China

• I’ve used VectorBuilder’s vector construction and lentivirus packaging services. The turnaround time was short and everything worked well. Also, I received quick responses from the customer support team.

  Xijuan Chen

  Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, China

• Thumbs up! I chose VectorBuilder after comparing it with other companies, because it provides high-quality products and services at unbeatable prices.

  Lihua Xu

  The First Affiliated Hospital of Guangzhou Medical University, China

• VectorBuilder did vector construction and lentivirus packaging for my shRNA knockdown experiment. The transduction efficiency of the lentivirus was high. Their staff were very considerate, and related technical questions were addressed quickly. I’ve finished many experiments and I’m preparing the manuscript for submission.

  Mengyuan Fan

  School of Nature Conservation, Beijing Forestry University, China

• We recently purchased a lentiviral plasmid for constitutive expression of GFP and luciferase and the corresponding lentivirus from VectorBuilder. We have infected several tumor cell lines with excellent results. The lentiviral stocks worked very nicely, saving us a lot of time. We are now using these modified cell lines for monitoring tumor growth in animal models using in vivo imaging.

  Javier Alonso

  Research institute for Rare Diseases, Spain