Vector Academy
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Keywords: ORF selection, MANE Select, Codon optimization, Expression vectors
Selecting the right ORF is more than choosing a coding sequence—it requires careful consideration of transcript isoforms, codon optimization, and vector design to achieve reliable expression.
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Keywords: Lentivirus vector, Age-related muscle loss, International Space Station
In an out-of-this-world experiment on the International Space Station (ISS), scientists from the University of Liverpool investigated skeletal muscle loss in microgravity, with implications for age-related muscle loss on earth. Researchers used 3D cultured muscle tissue that had been engineered using VectorBuilder’s lentiviral vector system.
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Keywords: Inducible gene expression, Tet-on, Tet-off, tetracycline
Explore inducible gene expression systems and learn how to choose the right Tet-inducible system to precisely control the expression of your target gene.
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Keywords: CRISPR library screen, in vitro and in vivo screens, antibiotic kill curve, lentivirus functional titration
Explore the critical steps in preparing and performing CRISPR screens and learn what to consider to set your screening project up for success.
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Keywords: Therapeutic development, cell and gene therapy, vector design
In today’s high-stakes cell and gene therapy (CGT) landscape, where capital is fleeting and timelines are tight, success favors those who treat vector candidate selection as a scalable science, not an academic gamble.
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Keywords: AAV, in vivo viral vector, therapeutic development, serotype tropism
Learn about AAV and the properties that have made it one of the most popular viral vectors for in vivo applications and therapeutic development.
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Keywords: CRISPR screening, gRNA library design, in vitro and in vivo models, library preparation
Explore the critical steps in designing and performing CRISPR screens and learn what to consider to set your screening project up for success.
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Keywords: mRNA vaccine, polyadenylation, poly(A) tail, mRNA stability
Learn about the crucial role of poly(A) tails in the life cycle of mRNAs and how to leverage this for optimal efficacy of mRNA-based therapeutics.
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Keywords: Gene delivery seminar series, iPSCs, neurodegenerative disease, CRISPR
Dr. Narayan's innovative work shows how patient-derived iPSC models are transforming our understanding of AD while paving the way toward targeted therapies.
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Keywords: CRISPR-Cas9, genome editing, CRISPR vector design, gene knockout, gene regulation
An overview of CRISPR-Cas9 tools and mechanisms, with practical tips to optimize vector design for efficient genome editing.
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Keywords: CRISPR delivery, plasmid, virus, mRNA, RNP
A comparison of delivery systems to help you choose the optimal method for your CRISPR experiments, from research applications to clinical use.
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Keywords: Lentivirus, Vector Design, Repetitive Sequences
Using two identical promoters in a lentiviral vector causes detrimental effects on proper expression of the two open reading frames.
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Keywords: Promoter, cloning, CMV, CAG, lentivirus, AAV
This article highlights the role of ubiquitous promoters in gene delivery, discussing the advantages and limitations of viral and non-viral promoters, including CMV, CAG, and EF1α, to guide you in your vector design decisions.
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Keywords: Oncolytic virus, tumor targeting, immune stimulation
A deep dive into the attributes and design of recombinant viruses for use as potent oncolytic agents in the clinic and why HSV and VACV are ideal oncolytic virus candidates.
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Keywords: Reporter genes, fluorescent reporters, luciferase assay
Discover primary reporter types and navigate the roadmap to find whether fluorescent reporters, luciferase, or beta-galactosidase is the best option to detect and measure your gene or element of interest.
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