Vector Academy
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Keywords: Reporter genes, fluorescent reporters, luciferase assay
Discover primary reporter types and navigate the roadmap to find whether fluorescent reporters, luciferase, or beta-galactosidase is the best option to detect and measure your gene or element of interest.
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Keywords: Bicistronic vector, 2A peptide, linker
This guide provides key considerations and troubleshooting tips for constructing vectors with multiple genes, including which linker to use and the importance of optimizing order.
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Keywords: Cre recombinase, conditional knockout, Cre-lox system
The Cre-Lox system, featuring Cre recombinase and loxP sites, is a cornerstone of genetic engineering, enabling precise control over gene expression and modification.
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Keywords: Transposable elements, transposase, PiggyBac
Learn about optimization strategies gene delivery systems using transposons and transposases. Here we compare different systems like piggyBac, Sleeping Beauty, and Tol2, detailing their integration mechanisms, carrying capacities, and target preferences.
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Keywords: CAR-T cell therapy, Kymriah CAR-T, immunotherpy
A deep dive into the biology behind CAR-T vectors, their clinical applications, and how CRISPR/Cas9 is being used to create the next generation of CAR therapeutics.
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Keywords: Cell culture media, plasmid yield, bacterial growth
Plasmid yield from bacterial cultures can be enhanced by optimizing antibiotics, culture conditions, and even the plasmid itself. This guide showcases how high your yield can be through simple steps.
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Keywords: VSV immunotherapy, oncolytic virus, pseudotyping
Vesicular stomatitis virus (VSV), known for its simplicity and well-explored biology, serves as an invaluable model in virology, particularly through pseudotyping, where its envelope protein is swapped with that of another virus.
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Keywords: Viral gene therapy, retrovirus, transient transfection
A comprehensive guide to selecting the appropriate viral vector for gene delivery experiments. The article examines how factors such as cell type, expression type, and insert size can impact your experiment while comparing the integration capabilities, carrying capacities, and tropisms of different viruses.
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Keywords: Zolgensma mechanism, spinal muscular atrophy
Learn about spinal muscular atrophy from a genetic perspective and the mechanisms behind two key gene therapy treatments, Spinraza and Zolgensma.
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Keywords: Viral titer, gene toxicity
If your viral titration is low, it could be because of titration method, improper vector or experimental design, or expression of toxic genes. Here we discuss troubleshooting all of these and more.
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Keywords: AAV packaging, AAV capsid engineering, AAV serotype tropism
Dive into the complexities of choosing the right AAV serotype for gene delivery, this guide elucidates the genetic and structural differences between serotypes, their tissue specificity, and evolution, ultimately guiding researchers in selecting the most suitable serotype for their experiments.
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Keywords: Viral vector, origin of replication, promoter
Learn about the components of a plasmid necessary for gene expression, including promoters, ORFs, polyA signals, selection markers, origins of replication, and antibiotic resistance genes.
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Keywords: Zynteglo gene therapy, beta thalassemia
Learn about the genetic blood disorder beta thalassemia and the lentiviral and CRISPR/Cas9-based gene therapy approaches for treatment.
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Keywords: Agarose gel electrophoresis, DNA conformation, ethidium bromide
A guide to how gel electrophoresis results are influenced by DNA conformation, ethidium bromide concentration, and method of introduction.
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Keywords: Muscular dystrophy, gutless adenovirus, gene therapy
Learn about exciting gene therapy approaches utilizing AAV, adenovirus and gutless adenovirus for treating Duchenne muscular dystrophy.
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