Vectors for eNpHR3.0/mCherry

Gene Information

  • Gene Name eNpHR3.0/mCherry
  • Type ORF
  • Description Third generation of Halorhodopsin from Natronomonas pharaonis (NpHR) which is fused with mCherry. NpHR is a fast light-activated electrogenic Cl- pump. There is an nAChR-derived signal peptide tagged to the N-terminal of mCherry, and a Kir2.1-derived ER export signal peptide tagged to the C-terminal of mCherry to facilitate cell surface expression of NpHR
  • Application Notes Light activation of NpHR leads to cell hyperpolarization and inhibition of firing action potentials; increased peak photocurrent compared to original NpHR; no aggregation; low cellular toxicity; maximum excitation is 580 nm; maximum excitation and emission of mCherry are 587 nm and 610 nm, respectively.
  • References Unpublished, provided by Karl Deisseroth through the UNC vector core
  • View ORF sequence of this gene »

Popular Vector Designs for eNpHR3.0/mCherry(These vectors can be made very quickly)

Gene Expression Vectors
These designs are created with VectorBuilder’s standard backbones containing a ubiquitous promoter driving the ORF of your gene. A marker (e.g. EGFP) is placed in the vector where appropriate.
Vector Description Vector Type Marker Price
eNpHR3.0/mCherry expression regular plasmid vector Mammalian Gene Expression Vector   Guide  From $159
eNpHR3.0/mCherry expression lentivirus vector Mammalian Gene Expression Lentiviral Vector   Guide  From $159
eNpHR3.0/mCherry expression adenovirus vector Mammalian Gene Expression Adenoviral Vector   Guide  From $159
eNpHR3.0/mCherry expression AAV vector Mammalian Gene Expression AAV Vector   Guide  From $159
eNpHR3.0/mCherry expression PiggyBac vector Mammalian Gene Expression PiggyBac Vector   Guide  From $159

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