5' ITR: 5' inverted terminal repeat. In wild type virus, 5' ITR and 3' ITR are essentially identical in sequence. They reside on two ends of the viral genome pointing in opposite directions, where they serve as the origin of viral genome replication.
Ψ: Adenovirus packaging signal required for the packaging of viral DNA into virus.
Promoter: The promoter that drives your gene of interest is placed here.
Kozak: Kozak consensus sequence. It is placed in front of the start codon of the ORF of interest because it is believed to facilitate translation initiation in eukaryotes.
ORF: The open reading frame of your gene of interest is placed here.
BGH pA: Bovine growth hormone polyadenylation. It facilitates transcriptional termination of the upstream ORF.
hPGK promoter: Human phosphoglycerate kinase 1 gene promoter. It drives the ubiquitous expression the downstream marker gene.
Marker: A visually detectable gene (such as EGFP). This allows cells transduced with the vector to be selected and/or visualized.
TK pA: Herpes simplex virus thymidine kinase polyadenylation signal. It facilitates transcriptional termination of the upstream ORF.
ΔAd5/F35: Portion of adenovirus serotype 5 genome with adenovirus serotype 35 fiber shaft and knob between the two ITRs minus the E1A, E1B and E3 regions; exhibits expanded tropism, especially for cells either completely lacking or having insufficient coxsackievirus and adenovirus receptor (CAR) expression.
3' ITR: 3' inverted terminal repeat.
pBR322 ori: pBR322 origin of replication. Plasmids carrying this origin exist in medium copy numbers in E. coli.
Ampicillin: Ampicillin resistance gene. It allows the plasmid to be maintained by ampicillin selection in E. coli.
PacI: PacI restriction site (PacI is a rare cutter that cuts at TTAATTAA). The two PacI restriction sites on the vector can be used to linearize the vector and remove the vector backbone from the viral sequence, which is necessary for efficient packaging.