3' ITR: 3' inverted terminal repeat. In wild type virus, 5' ITR and 3' ITR are essentially identical in sequence. They reside on two ends of the viral genome pointing in opposite directions, where they serve as the origin of viral genome replication.
Ad5_E4 fragment: Adenovirus serotype 5 E4 gene promoter region. Can improve vector stability and supports packaging.
Promoter: The promoter that drives your gene of interest is placed here.
Kozak: Kozak consensus sequence. It is placed in front of the start codon of the ORF of interest because it is believed to facilitate translation initiation in eukaryotes.
ORF: The open reading frame of your gene of interest is placed here.
BGH pA: Bovine growth hormone polyadenylation signal. It facilitates transcriptional termination of the upstream ORF.
hPGK promoter: Human phosphoglycerate kinase 1 gene promoter. It drives the ubiquitous expression the downstream marker gene.
Marker: A visually detectable gene (such as EGFP). This allows cells transduced with the vector to be selected and/or visualized.
TK pA: Herpes simplex virus thymidine kinase polyadenylation signal. It facilitates transcriptional termination of the upstream ORF.
C346_Stuffer: Part of C346 cosmid (GenBank L31948) DNA sequence. Used for maintaining a final size above 28 kb between the two ITRs of vectors to facilitate efficient packaging into virions.
HPRT_Stuffer: Part of human hypoxanthine-guanine phosphoribosyltransferase (HPRT1) DNA sequence (containing introns 1, 2 and 3 and exon 2, 3). Used for maintaining a final size above 28 kb between the two ITRs of vectors to facilitate efficient packaging into virions.
Ψ: Adenovirus packaging signal required for the packaging of viral DNA into virus.
5' ITR: 5' inverted terminal repeat. See description for 3’ ITR.
Kanamycin: Kanamycin resistance gene. It allows the plasmid to be maintained by kanamycin selection in E. coli.
pBR322 ori: pBR322 origin of replication. Plasmids carrying this origin exist in medium copy numbers in E. coli.