Promoter: The promoter driving your gene of interest is placed here.
Kozak: Kozak consensus sequence. This is placed in front of the start codon of the ORF of interest to facilitate translation initiation in eukaryotes.
ORF: The open reading frame of your gene of interest is placed here.
Nos pA: The nopaline synthase polyadenylation signal of Agrobacterium tumefaciens. This facilitates transcription termination of the upstream ORF.
RB T-DNA repeat: Right border repeat of T-DNA. Upon recognized by Ti plasmid in Agrobacterium, the region between the T-DNA border repeats is transferred to plant cells.
pVS1 StaA: Stability protein from the plasmid pVS1. Essential for stable plasmid segregation in Agrobacterium.
pVS1 RepA: Replication protein from the plasmid pVS1. Permits replication of low-copy plasmids in Agrobacterium.
pVS1 oriV: Origin of replication from the plasmid pVS1. Permits replication of low-copy plasmids in Agrobacterium.
pBR322 ori: pBR322 origin of replication. Facilitates plasmid replication in E. coli. Plasmids carrying this origin exist in low copy numbers (15-20 per cell) in E. coli if Rop protein is present, or medium copy numbers (100-300 per cell) if Rop protein is absent.
Kanamycin: Kanamycin resistance gene. It allows the plasmid to be maintained by kanamycin selection in bacterial hosts.
LB T-DNA repeat: Left border repeat of T-DNA. Upon recognized by Ti plasmid in Agrobacterium, the region between the T-DNA border repeats is transferred to plant cells.
CaMV 35S_enhanced: A strong chimeric promoter which drives marker expression.
CaMV 35S pA: Cauliflower mosaic virus 35S polyadenylation signal. This facilitates transcription termination and polyadenylation of the marker gene.
Marker: A drug selection gene, allowing selection of plant cells transduced with the vector.