Enhancer: Your enhancer of interest is placed here.
Hsp68 minimal promoter: The minimal promoter sequence from mouse Hsp68 (heat shock protein 68kDa). This will drive transcription of the reporter if an enhancer element is present to activate it. In the absence of such enhancer activity, the minimal promoter will be almost completely inactive.
LacZ: The beta-galactosidase reporter gene. The encoded enzyme converts the colorless and soluble X-gal to an intensely blue insoluble product that stains the cells in which LacZ is expressed.
SV40 early pA: Simian virus 40 early polyadenylation signal. It facilitates transcriptional termination of the upstream ORF.
Ampicillin: Ampicillin resistance gene. It allows the plasmid to be maintained by ampicillin selection in E. coli.
pUC ori: pUC origin of replication. Plasmids carrying this origin exist in high copy numbers in E. coli.