TRE promoter: Tetracycline-responsive element promoter. This element can be regulated by a class of transcription factors (e.g. tTA, rtTA and tTS) whose activities are dependent on tetracycline or its analogs (e.g. doxycycline).
Kozak: Kozak consensus sequence. It is placed in front of the start codon of the ORF of interest to facilitate translation initiation in eukaryotes.
ORF: The open reading frame of your gene of interest is placed here.
rBG pA: Rabbit beta-globin polyadenylation signal. It facilitates transcriptional termination of the upstream ORF.
Promoter: The tissue-specific promoter chosen to drive expression of the rtTA protein.
rtTA: Reverse tetracycline responsive transcriptional activator M2. This protein binds to TRE promoter to activate gene transcription only in the presence of tetracycline or its analogs (e.g. doxycycline). It has higher sensitivity to the inducing drug and lower leaky activity in the absence of the drug compared to its predecessor.
SV40 late pA: Simian virus 40 late polyadenylation signal. It facilitates transcriptional termination of the upstream rtTA protein.
CBh promoter: CMV early enhancer fused to modified chicken β-actin promoter. This drives the expression of the downstream tTS protein.
tTS: Tetracycline-controlled transcriptional silencer. This protein binds to TRE promoter to actively suppress gene transcription only in the absence of tetracycline and its analogs (e.g. doxycycline).
SV40 late pA: Simian virus 40 late polyadenylation signal. It facilitates transcriptional termination of the upstream tTS protein.
Ampicillin: Ampicillin resistance gene. It allows the plasmid to be maintained by ampicillin selection in E. coli.
pUC ori: pUC origin of replication. Plasmids carrying this origin exist in high copy numbers in E. coli.