VectorBuilder offers many popular vector components that users can choose from when designing their vectors. The tables below provide detailed information on these popular components, which are listed separately by category.
CAR-T Domains
| Name | Description | Application Notes | Length | Details |
|---|---|---|---|---|
| CD8-leader | Leader signal peptide of T-cell surface glycoprotein CD8 alpha chain | Directs transport and localization of the protein to the T-cell surface. |
60 bp |
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| CD8-hinge | Hinge region of T-cell surface glycoprotein CD8 alpha chain | Connects scFv and the transmembrane region; enhances tumor recognition efficiency; improves the expansion of CAR-T cells. |
144 bp |
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| CD28-hinge | Hinge region of the T-cell surface glycoprotein CD28 | Connects scFv with the transmembrane region providing stability and flexibility for efficient CAR expression and function; enhances efficiency of tumor recognition; improves expansion of CAR-T cells. |
117 bp |
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| CD8-TM | Transmembrane region of T-cell surface glycoprotein CD8 alpha chain | Anchors the CAR to the plasma membrane and bridges the extracellular hinge as well as antigen recognition domains with the intracellular signaling region; enhances receptor expression and stability. |
72 bp |
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| CD28-TM | Transmembrane region of T-cell-surface glycoprotein CD28 | Anchors the CAR to the plasma membrane and bridges the extracellular hinge as well as antigen recognition domains with the intracellular signaling region; enhances receptor expression and stability. |
81 bp |
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Stimulatory Domains
| Name | Description | Application Notes | Length | Details |
|---|---|---|---|---|
| CD3zeta | Intracellular domain of the T cell receptor-CD3ζ chain | Acts as a stimulatory molecule for activating T cell-mediated immune response. |
336 bp |
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Costimulatory Domains
| Name | Description | Application Notes | Length | Details |
|---|---|---|---|---|
| CD28 | CD28 intracellular costimulatory domain | Improves overall survival, proliferation and persistence of activated CAR-T cells. |
123 bp |
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| 4-1BB | 4-1BB intracellular costimulatory domain | Improves overall survival, proliferation and persistence of activated CAR-T cells; further enhances cytotoxicity and durability when used in combination with CD28. |
126 bp |
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scFv
| Name | Description | Application Notes | Length | Details |
|---|---|---|---|---|
| CD19-scFv | Single-chain variable fragment derived from CD19 antibody | Recognizes human CD19-expressing cells in an antigen-specific manner. |
744 bp |
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